Direct organogenesis in vitro propagation of local balinese banana with thidiazuron

  • I Nyoman Rai Udayana University, Denpasar, Indonesia
  • I Made Sudana Udayana University, Denpasar, Indonesia
  • I Nyoman Gede Astawa Udayana University, Denpasar, Indonesia
  • Rindang Dwiyani Udayana University, Denpasar, Indonesia
  • Yuyun Fitriani Udayana University, Denpasar, Indonesia
Keywords: in vitro, local Balinese banana, organogenesis, propagation, thidiazuron

Abstract

Bali’s community needs for bananas are very high, but cannot be fulfilled from the production of bananas in Bali. The main problem for banana cultivation in Bali is the difficulty of getting seeds.  Direct organogenesis is offered to overcome this problem.  The objective of the research was to study direct organogenesis on in vitro propagation of local Balinese banana with Thidiazuron. The research was carried out at Tissue Culture Laboratory, Faculty of Agriculture, Udayana University, Denpasar, Bali. The experiment was laid out in a completely randomized design with five treatments of thidiazuron concentration, i.e. 0; 0.5; 1.0; 1.5, and 2.0 ppm. Each treatment was replicated 5 times. The Murashige & Skoog medium added with coconut water and extract of bamboo shoots was used as a basic medium.   The local Balinese bananas named Saba and Susu were used as source of planting materials.  Explants were taken from shoot tips of suckers of newly growing plants. The results showed that the best treatment for both Saba and Susu, was 1 ppm of thidiazuron, as indicated by the highest number of plantlets produced, the number of shoots and plant height.

Downloads

Download data is not yet available.

References

Alatar, A. A. (2015). Thidiazuron induced efficient in vitro multiplication and ex vitro conservation of Rauvolfia serpentina–a potent antihypertensive drug producing plant. Biotechnology & Biotechnological Equipment, 29(3), 489-497. https://doi.org/10.1080/13102818.2015.1017535

Ali, S., & Mehmood, S. (2017). Micro Propagation of cv. Basrai (Banana) Using Growth Hormones. J Hortic, 4(195), 2376-0354. https://doi.org/10.4172/2376-0354.1000195

Astiari, N. K. A., Kartini, L., Sulistiawati, N. P. A., & Rai, I. N. (2018). Efforts to produce Siamese citrus fruit out of season and fruit quality improvement through application of potassium nitrate and agrodyke fertilizer. International Journal of Life Sciences, 2(3), 48-58. https://doi.org/10.29332/ijls.v2n3.206

Bandita, D., & Bikram, P. (2017). Effects of plant growth hormones on shoot proliferation of Musa paradisiaca cv. BANTAL. International Journal of Plant Sciences (Muzaffarnagar), 12(2), 135-138. http://dx.doi.org/10.15740/has/ijps/12.2/135-138

Bhojwani, S. S., & Razdan, M. K. (1996). Plant tissue culture, theory and practices. A revised edition. Studies in plant science, 5.

Convention on Biological Diversity. (2017). Indonesia-Country Profile. https://www.cbd.int/countries/profile/default.shtml?country=id. Montreal, Canada. Accessed on 22/09/2017

Dahot, M. U. (2007). Micro-propagation efficiency in banana (Musa sp.) under different immersion systems. Pakistan journal of biological sciences: PJBS, 10(5), 726-733. https://doi.org/10.3923/pjbs.2007.726.733

Dwiyani, R. (2015). Kultur Jaringan Tanaman. Pelawa Sari, Denpasar.

Eriansyah, M., Susiyanti, S., & Putra, Y. (2018). Pengaruh Pemotongan Eksplan Dan Pemberian Beberapa Konsentrasi Air Kelapa Terhadap Pertumbuhan Dan Perkembangan Eksplan Pisang Ketan (Musa paradisiaca) Secara In Vitro. Agrologia, 3(1). http://dx.doi.org/10.30598/a.v3i1.260

Faisal, M., Ahmad, N., & Anis, M. (2005). Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell, Tissue and Organ Culture, 80(2), 187-190. https://doi.org/10.1007/s11240-004-0567-x

FAO (Food and Agriculture Organization). 2018. Post-harvest management of banana for quality and safety assurance. Guidance for horticultural supply chain stakeholders. Food and Agriculture Organization of the United Nations (FAO). Viale delle Terme di Caracalla 00153 Rome, Italy. www.fao.org. 24p.

Husaini, A. M., Mercado, J. A., da Silva, J. A. T., & Schaart, J. G. (2011). Review of factors affecting organogenesis, somatic embryogenesis and Agrobacterium tumefaciens-mediated transformation of strawberry. Genes, Genomes and Genomics (Special Issue I), 5, 1-11.

Israeli, Y., Lahav, E., & Reuveni, O. (1995). In vitro culture of bananas. In Bananas and plantains (pp. 147-178). Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0737-2_6

Jain, P., Jain, A., Singhai, R., & Jain, S. (2017). Effect of bio-degradation and non degradable substances in environment. International Journal of Life Sciences, 1(1), 58-64. https://doi.org/10.21744/ijls.v1i1.24

Kishor, H., Abhijith, Y. C., & Manjunatha, N. (2017). Micropropagation of native cultivars of Banana-A Critical Review. International Journal of Pure and Applied Bioscience, 5(5), 1559-1564.

Latunra, A. I. (2018). Induksi Kalus Pisang Barangan Merah Musa acuminata Colla dengan Kombinasi Hormon 2, 4-D dan Bap Secara In Vitro. Jurnal Ilmu Alam dan Lingkungan, 8(1).

Linda, R. Perbanyakan Tunas Pisang Cavendish (Musa acuminata L.) Secara In Vitro dengan Penambahan Naphthalene Acetic Acid (NAA) dan Air Kelapa. Protobiont, 7(1).

Lohidas, J., & Sujin, D. (2015). Effect of growth hormones in the micro propagation of banana CV. Matti. Plant Archives, 15(1), 307-314.

Lu, C. Y. (1993). The use of thidiazuron in tissue culture. In Vitro Cellular & Developmental Biology-Plant, 29(2), 92-96. https://doi.org/10.1007/BF02632259

Masekesa, T. R., Gasura, E., Ngadze, E., Icishahayo, D., Kujeke, G. T., Chidzwondo, F., & Robertson, I. (2016). Efficacy of Zeatin, Kinetin and Thidiazuron in induction of adventitious root and shoot from petiole explants of sweetpotato cv. Brondal. South African Journal of Botany, 104, 1-5. https://doi.org/10.1016/j.sajb.2015.11.001

Mose, W., Indrianto, A., Purwantoro, A., & Semiarti, E. (2017). The influence of thidiazuron on direct somatic embryo formation from various types of explant in Phalaenopsis amabilis (L.) blume orchid. HAYATI Journal of Biosciences, 24(4), 201-205. https://doi.org/10.1016/j.hjb.2017.11.005

Muhammad, A., Hussain, I. Q. B. A. L., Naqvi, S. S., & Rashid, H. (2004). Banana plantlet production through tissue culture. Pakistan Journal of Botany, 36(3), 617-620.

Ngomuo, M., Mneney, E., & Ndakidemi, P. (2013). The effects of auxins and cytokinin on growth and development of (Musa sp.) var.“Yangambi” explants in tissue culture. American Journal of Plant Sciences, 4(11), 2174. http://dx.doi.org/10.4236/ajps.2013.411269

Nuwagira, F., Mukasa, S. B., Wagoire, W. W., Namugga, P., Kashaija, I. N., & Barekye, A. (2015). Determination of hormonal combination for increased multiplication of tissue culture potato plantlets. Uganda Journal of Agricultural Sciences, 16(1), 129-137. http://dx.doi.org/10.4314/ujas.v16i1.11

Ogu, G. I., & Orjiakor, P. I. (2017). Microbiological and nutritional qualities of fermented melon seed shells. International Journal of Life Sciences, 1(2), 1-9. https://doi.org/10.21744/ijls.v1i2.27

Opabode, J. T. (2017). Sustainable mass production, improvement, and conservation of African indigenous vegetables: The role of plant tissue culture, a review. International Journal of Vegetable Science, 23(5), 438-455. https://doi.org/10.1080/19315260.2017.1319006

Prabawati, S., Suyanti, S., & Setyabudi, D. A. (2008). Teknologi Pascapanen dan Pengolahan Buah Pisang. In Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian. Seminar Badan Litbang Pertanian. Departemen Pertanian. Bogor.

Prabhuling, G., & Sathyanarayana, B. N. (2017). Optimization of explants density for tissue culture propagation of banana cv.‘Grande Naine’. International Journal of Agricultural Sciences, 13(1), 71-76. https://doi.org/10.15740/HAS/IJAS/13.1/71-76

Rai, I N., I M. Sudana, I M. Sukewijaya, I N.G. Ustriyana, I D.P. Oka Suardi, G.N.A. Susanta Wirya, D.N. Nyana. (2018b). Pengembangan Aneka Jenis Pisang di Kabupaten Gianyar. Laporan Akhir Kerjasama Dinas Pertanian, Kabupaten Gianyar Dengan Tim Fakultas Pertanian Universitas Udayana, 56.

Rai, I. N., Dwivany, F. M., Sutanto, A., Meitha, K., Sukewijaya, I. M., & Ustriyana, I. N. G. (2018). Biodiversity of Bali Banana (Musaceae) and its Usefulness. Hayati Journal of Biosciences, 25(2), 47. https://doi.org/10.4308/hjb.25.2.47

Rai, I.N., I.W. Nuarsa, I.G.N. Alit Susanta Wirya, I.N.G. Ustriyana, D.P. Oka Suardi, M. Sukewijaya, K. Wikantika, F.M. Dwivany, K. Meitha. (2017). Studi Biogeografi dan Biodiversity Pisang Bali. Laporan Akhir Kegiatan Penelitian Bali International Research Center For Banana (BIRCB), 151.

Rivai, R. R., & Helmanto, H. E. N. D. R. A. (2015). Induksi kalus Crhysanthemum indicum untuk meningkatkan keragaman genetic dari sel somatic. pros sem nas masy biodiv indon, 1(1), 167-170.

Sharma, V., Gupta, S. K., & Dhiman, M. (2012). Regeneration of plants from nodal and internodal segment cultures of Ephedra gerardiana using thidiazuron. Plant Tissue Culture and Biotechnology, 22(2), 153-161. https://doi.org/10.3329/ptcb.v22i2.14205

Sheil, D., Puri, R. K., Basuki, I., van Heist, M., Wan, M., Liswanti, N., ... & Angi, E. M. (2002). Exploring biological diversity, environment, and local people's perspectives in forest landscapes: Methods for a multidisciplinary landscape assessment. CIFOR.

Singh, C. R. (2018). Review on Problems and its Remedy in Plant Tissue Culture. Asian Journal of Biological Sciences, 11, 165-172. https://doi.org/10.3923/ajbs.2018

Suiraoka, I. P., Duarsa, D. P. P., Wirawan, I. D. N., & Bakta, I. M. (2017). Perception of parents, teachers, and nutritionist on childhood obesity and barriers to healthy behavior: a phenomenological study. International Journal of Health Sciences, 1(2), 1-11. https://doi.org/10.21744/ijhs.v1i2.25

Tumuhimbise, R., & Talengera, D. (2018). Improved Propagation Techniques to Enhance the Productivity of Banana (Musa spp.). Open Agriculture, 3(1), 138-145. https://doi.org/10.1515/opag-2018-0014

Published
2019-11-23
How to Cite
Rai, I. N., Sudana, I. M., Astawa, I. N. G., Dwiyani, R., & Fitriani, Y. (2019). Direct organogenesis in vitro propagation of local balinese banana with thidiazuron. International Journal of Life Sciences, 3(3), 32-40. https://doi.org/10.29332/ijls.v3n3.360
Section
Articles