Sequencing of ca-int-l gene of Candida Spp. In infected urinary tract among Iraqi women
Keywords:
Sequencing, urinary, among IraqiAbstract
The most common nosocomial fungal infection in hospitals is urinary tract candidiasis. Candida albicans is the most prevalent cause of nosocomial fungal urinary tract infections, however Candida species distribution is changing rapidly. At the same time, the rise in urinary tract candidiasis has resulted in the emergence of antifungal-resistant Candida species. This study aimed to diagnose Candida Spp. In women with UTI and reveal the nucleotides sequences of CA-INT-L Gene to look for mutation within the gene. This study included 100 women patients suffering from urinary tract infections and vaginal swabs samples from those individuals were taken to identify the presence of Candida. They were between the ages of 22 and 67. Candida identification has been accomplished using a variety of chemical and microscopically techniques. 100 samples have been clinically diagnosed with Candida Spp. But only 62 of the samples were positive by Vitik. This number has also reduced to only 25 after using the PCR. The sequence of the CA-INT-L showed different variations within the gene of the isolates.
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• Ford, C. B., Funt, J. M., Abbey, D., Issi, L., Guiducci, C., Martinez, D. A., Delorey, T., Li, B. Y., White, T. C., Cuomo, C., Rao, R. P., Berman, J., Thompson, D. A., & Regev, A. (2015). The evolution of drug resistance in clinical isolates of Candida albicans. eLife, 4, e00662. https://doi.org/10.7554/eLife.00662
• Arya N. R, and Naureen B. Rafiq. “Candidiasis - StatPearls - NCBI Bookshelf.” https://www.ncbi.nlm.nih.gov/books/NBK560624/ (March 27, 2022).
• Basmaciyan, Louise et al. 2019. “‘Candida Albicans Interactions With The Host: Crossing The Intestinal Epithelial Barrier.’” Tissue Barriers 7(2): 1612661. /pmc/articles/PMC6619947/ (March 27, 2022).
• Embong, Zunaina et al. 2008. “Specific Detection of Fungal Pathogens by 18S RRNA Gene PCR in Microbial Keratitis.” BMC Ophthalmology 8(1): 1–8. https://bmcophthalmol.biomedcentral.com/articles/10.1186/1471-2415-8-7 (April 21, 2022).
• Jiang, Xiaoqun et al. 2016. “Rapid Detection of Candida Albicans by Polymerase Spiral Reaction Assay in Clinical Blood Samples.” Frontiers in Microbiology 7(JUN): 916.
• Kim, Sang Jin et al. 2019. “Management of Candida Urinary Tract Infection in the Elderly.” Urogenit Tract Infect 14(2): 33–2019. http://crossmark.crossref.org/dialog/?doi
• =10.14777/uti (March 27, 2022).
• Hamied, A. S. (2021) Candida and Candidiasis – A review of Virulence Factors . J. Biotechno. Res. Cen. Vol.15(2) : 13 – 20 PP.
• Kogan, Mikhail, Carlos Cuellar Castillo, and Melissa S. Barber. 2016. “Chronic Rhinosinusitis and Irritable Bowel Syndrome: A Case Report.” Integrative Medicine: A Clinician’s Journal 15(3): 44. /pmc/articles/PMC4982648/ (March 27, 2022).
• Schuster, Jennifer E., and Brian T. Fisher. 2022. “Candidiasis.” Pediatric Transplant and Oncology Infectious Diseases: 195-205.e3. https://www.ncbi.nlm.nih.gov/books/NBK560624/ (March 27, 2022).
• Mohsin, Z. A. and Ali, W. Sh. (2021) . Antagonistic Activity of Bacteriocin-producing Lactobacillus Against Candida spp . Iraqi J. Sci. Vol. 62(7) : 2153 – 2162 PP.
• Othman, K. I. , Abdullah, Sh. M. , Ali, B. and Majid, M. (2018) . Isolation and Identification Candida spp from Urine and Antifungal
• Susceptibility Test . Iraqi J. Sci. Vol. 59(4B) : 1981 – 1988 PP.
• He, Zheng-Xin, Zhao, Hui-Hai and Wang, Fu-Kun. "PCR-detectable Candida DNA exists a short period in the blood of systemic candidiasis murine model" Open Life Sciences, vol. 15, no. 1, 2020, pp. 677-682
• Edmond M. B., et al. 1999. Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin. Infect. Dis. 29:239–244
• Horn D. L., et al. 2009. Epidemiology and outcomes of candidemia in 2019 patients: data from the prospective antifungal therapy alliance registry. Clin. Infect. Dis. 48:1695–1703
• Jalil, A. T., Kadhum, W. R., Khan, M. U. F., Karevskiy, A., Hanan, Z. K., Suksatan, W., ... & Abdullah, M. M. (2021). Cancer stages and demographical study of HPV16 in gene L2 isolated from cervical cancer in Dhi-Qar province, Iraq. Applied Nanoscience, 1-7. https://doi.org/10.1007/s13204-021-01947-9
• Widjaja, G., Jalil, A. T., Rahman, H. S., Abdelbasset, W. K., Bokov, D. O., Suksatan, W., ... & Ahmadi, M. (2021). Humoral Immune mechanisms involved in protective and pathological immunity during COVID-19. Human Immunology. https://doi.org/10.1016/j.humimm.2021.06.011
• Moghadasi, S., Elveny, M., Rahman, H. S., Suksatan, W., Jalil, A. T., Abdelbasset, W. K., ... & Jarahian, M. (2021). A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine. Journal of Translational Medicine, 19(1), 1-21. https://doi.org/10.1186/s12967-021-02980-6
• Saleh, M. M., Jalil, A. T., Abdulkereem, R. A., & Suleiman, A. A .(2020). Evaluation of Immunoglobulins, CD4/CD8 T Lymphocyte Ratio and Interleukin-6 in COVID-19 Patients. TURKISH JOURNAL of IMMUNOLOGY, 8(3), 129-134. https://doi.org/10.25002/tji.2020.1347
• Turki Jalil, A., Hussain Dilfy, S., Oudah Meza, S., Aravindhan, S., M Kadhim, M., & M Aljeboree, A. (2021). CuO/ZrO2 nanocomposites: facile synthesis, characterization and photocatalytic degradation of tetracycline antibiotic. Journal of Nanostructures. https://doi.org/10.22052/JNS.2021.02.014
• Sarjito, Elveny, M., Jalil, A., Davarpanah, A., Alfakeer, M., Awadh Bahajjaj, A. & Ouladsmane, M. (2021). CFD-based simulation to reduce greenhouse gas emissions from industrial plants. International Journal of Chemical Reactor Engineering, 20210063. https://doi.org/10.1515/ijcre-2021-0063
• Marofi, F., Rahman, H. S., Al-Obaidi, Z. M. J., Jalil, A. T., Abdelbasset, W. K., Suksatan, W., ... & Jarahian, M. (2021). Novel CAR T therapy is a ray of hope in the treatment of seriously ill AML patients. Stem Cell Research & Therapy, 12(1), 1-23. https://doi.org/10.1186/s13287-021-02420-8
• Jalil, A. T., Shanshool , M. T. ., Dilfy, S. H. ., Saleh, M. M., & Suleiman, A. A. . (2022). HEMATOLOGICAL AND SEROLOGICAL PARAMETERS FOR DETECTION OF COVID-19. Journal of Microbiology, Biotechnology and Food Sciences, e4229. https://doi.org/10.15414/jmbfs.4229
• Vakili-Samiani, S., Jalil, A. T., Abdelbasset, W. K., Yumashev, A. V., Karpisheh, V., Jalali, P., ... & Jadidi-Niaragh, F. (2021). Targeting Wee1 kinase as a therapeutic approach in Hematological Malignancies. DNA repair, 103203. https://doi.org/10.1016/j.dnarep.2021.103203
• NGAFWAN, N., RASYID, H., ABOOD, E. S., ABDELBASSET, W. K., Al-SHAWI, S. G., BOKOV, D., & JALIL, A. T. (2021). Study on novel fluorescent carbon nanomaterials in food analysis. Food Science and Technology. https://doi.org/10.1590/fst.37821
• Marofi, F., Abdul‐Rasheed, O. F., Rahman, H. S., Budi, H. S., Jalil, A. T., Yumashev, A. V., ... & Jarahian, M. (2021). CAR‐NK cell in cancer immunotherapy; A promising frontier. Cancer Science, 112(9), 3427. https://doi.org/10.1111/cas.14993
• Abosaooda, M., Wajdy, J. M., Hussein, E. A., Jalil, A. T., Kadhim, M. M., Abdullah, M. M., ... & Almashhadani, H. A. (2021). Role of vitamin C in the protection of the gum and implants in the human body: theoretical and experimental studies. International Journal of Corrosion and Scale Inhibition, 10(3), 1213-1229. https://dx.doi.org/10.17675/2305-6894-2021-10-3-22
• Jumintono, J., Alkubaisy, S., Yánez Silva, D., Singh, K., Turki Jalil, A., Mutia Syarifah, S., ... & Derkho, M. (2021). Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4° C for 50 Hours. Archives of Razi Institute, 76(4), 923-931. https://dx.doi.org/10.22092/ari.2021.355901.1735
• Raya, I., Chupradit, S., Kadhim, M. M., Mahmoud, M. Z., Jalil, A. T., Surendar, A., ... & Bochvar, A. N. (2021). Role of Compositional Changes on Thermal, Magnetic and Mechanical Properties of Fe-PC-Based Amorphous Alloys. Chinese Physics B. https://doi.org/10.1088/1674-1056/ac3655
• Chupradit, S., Jalil, A. T., Enina, Y., Neganov, D. A., Alhassan, M. S., Aravindhan, S., & Davarpanah, A. (2021). Use of Organic and Copper-Based Nanoparticles on the Turbulator Installment in a Shell Tube Heat Exchanger: A CFD-Based Simulation Approach by Using Nanofluids. Journal of Nanomaterials. https://doi.org/10.1155/2021/3250058
• Mohaddeseh Rahbaran, Ehsan Razeghian, Marwah Suliman Maashi, Abduladheem Turki Jalil, Gunawan Widjaja, Lakshmi Thangavelu, Mariya Yurievna Kuznetsova, Pourya Nasirmoghadas, Farid Heidari, Faroogh Marofi, Mostafa Jarahian, "Cloning and Embryo Splitting in Mammalians: Brief History, Methods, and Achievements", Stem Cells International, vol. 2021, Article ID 2347506, 11 pages, 2021. https://doi.org/10.1155/2021/2347506
• Jalil, A.T.; Ashfaq, S.; Bokov, D.O.; Alanazi, A.M.; Hachem, K.; Suksatan, W.; Sillanpää, M. High-Sensitivity Biosensor Based on Glass Resonance PhC Cavities for Detection of Blood Component and Glucose Concentration in Human Urine. Coatings 2021, 11, 1555. https://doi.org/10.3390/coatings11121555
• Chupradit, S.; Ashfaq, S.; Bokov, D.; Suksatan, W.; Jalil, A.T.; Alanazi, A.M.; Sillanpaa, M. Ultra-Sensitive Biosensor with Simultaneous Detection (of Cancer and Diabetes) and Analysis of Deformation Effects on Dielectric Rods in Optical Microstructure. Coatings 2021, 11, 1564. https://doi.org/10.3390/coatings11121564
• Bassetti M., et al. 2007. Incidence, risk factors, and predictors of outcome of candidemia. Survey in 2 Italian university hospitals. Diagn. Microbiol. Infect. Dis. 58:325–331
• Avni T, Leibovici L, Paul M. PCR diagnosis of invasive candidiasis: systematic review and meta-a
• Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
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