An in vitro study of apical bacterial extrusion after root canal instrumentation using several endodontic file systems
Keywords:
Fusobacterium nucleatum, edge taper platinum, Hyflex CM, K3XAbstract
Aim: In this research, the amount of apical extrusion of bacteria will be measured utilizing root canal instruments, including the K3XF, Protaper Gold, Edge taper Platinum, and Hyflex CM Rotary devices. Materials and Procedures: The saline solution contained sixty recently removed maxillary incisor teeth. After the trenches had been dug, the mash and bacteria were removed and placed in the entry pit to be used. In order to facilitate the gathering of germs, the teeth were attached to a device. Fusobacterium Nucleatum (ATCC 25586) was used to taint and then dry the root trenches for 24 hours. Six meetings made use of various tape systems, including K3XF edge tighten platinum and Hyflex CM turning reports, while the first four made use of hand-written K-records. Group 1 lacked any instrumentation (the control pack). The number of state molding units was tallied after 24 hours of Mueller-Hinton agar testing. The Kruskal-Wallis and Mann-Whitney U tests were used to analyse the data individually. Results: The K3XF file system emitted the fewest germs as compared to the other four rotating systems. Conclusion: Bacteria from intracanal spaces were ejected apically by all instrumentation methods.
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Alani MA, Al-Huwaizi H. Evaluation of apically extruded debris and irrigants during root canal preparation using different rotary instrumentation systems: An in vitro comparative study. Int J Med Res Health Sci. 2019;8:21–6.
al-Omari MA, Dummer PM. Canal blockage and debris extrusion with eight preparation techniques. J Endod. 1995;21:154–8.
Baker NA, Eleazer PD, Averbach RE, Seltzer S. Scanning electron microscopic study of the efficacy of various irrigating solutions. J Endod. 1975;1:127–35.
Batara, R., Ardani, I. G. A. I., Wardani, I. A. K., Windiani, I. G. A. T., Adnyana, I. G. A. N. S., & Setiawati, Y. (2022). Psychogenic dysphagia in children, and the success of family-based treatment: Case report. International Journal of Health & Medical Sciences, 5(1), 163-168. https://doi.org/10.21744/ijhms.v5n1.1894
Beeson TJ, Hartwell GR, Thornton JD, Gunsolley JC. Comparison of debris extruded apically in straight canals: Conventional filing versus profile 0.04 Taper series 29. J Endod. 1998;24:18–22.
Bolstad AI, Jensen HB, Bakken V. Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microbiol Rev. 1996;9:55–71.
Capar ID, Arslan H, Akcay M, Ertas H. An in vitro comparison of apically extruded debris and instrumentation times with ProTaper Universal, ProTaper Next, Twisted File Adaptive, and HyFlex instruments. J Endod. 2014;40:1638–41.
Chávez de Paz Villanueva LE. Fusobacterium nucleatum in endodontic flare-ups. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;93:179–83.
Collins CH, Lyne PM, Grange JM. Counting methods. In: Collins CH, Lyne PM, Grange JM, editors. Collins‘ and Lyne‘s Microbiological Methods. 7th ed. Oxford, London: Butterworth Heinemann; 1995. pp. 149–62.
Engstrom B. The significance of enterococci in root canal treatment. Odontoligisk Revy. 1964;15:87–106.
Er K, Sümer Z, Akpinar KE. Apical extrusion of intracanal bacteria following use of two engine-driven instrumentation techniques. Int Endod J. 2005;38:871–6.
Fairbourn DR, McWalter GM, Montgomery S. The effect of four preparation techniques on the amount of apically extruded debris. J Endod. 1987;13:102–8.
Ghogre P, Chourasia HR, Agarwal M, Singh MP, Gurav S, Ghogre R. Quantitative evaluation of apical extrusion of intracanal bacteria using rotary ProTaper, K3XF, twisted and hand K-file system: An ex vivo study. Indian J Dent Res. 2015;26:406–10.
Goerig AC, Michelich RJ, Schultz HH. Instrumentation of root canals in molar using the step-down technique. J Endod. 1982;8:550–4.
Hegde MN, Thatte S. Comparison of the amount of apical extrusion of bacteria following the use of different instrumentation techniques – An in vitro study. NUJHS. 2011;1:27–32.
Labbaf H, Nazari Moghadam K, Shahab S, Mohammadi Bassir M, Fahimi MA. An in vitro comparison of apically extruded debris using reciproc, protaper universal, neolix and hyflex in curved canals. Iran Endod J. 2017;12:307–11.
Miharja, M. ., Setiawati, S. ., & Lubis, A. L. P. . (2020). How dangerous the Indonesian recession due to COVID-19 pandemic: review policy and strategy to recovery. International Journal of Social Sciences and Humanities, 4(3), 121–129. https://doi.org/10.29332/ijssh.v4n3.470
Mittal R, Singla MG, Garg A, Dhawan A. A comparison of apical bacterial extrusion in manual, ProTaper rotary, and one shape rotary instrumentation techniques. J Endod. 2015;41:2040–4.
Mohammed N, Noushad MC, Balan B, Dhanesh N, Jayasheelan N, Revankar VD. Apical extrusion of intracanal bacteria following use of two engine-driven instrumentation techniques: An in vitro study. J Contemp Dent Pract. 2016;17:939–42.
Moraes SR, Siqueira JF, Jr, Colombo AP, Rocas ID, Ferreira MC, Domingues RM. Comparison of the effectiveness of bacterial culture, 16S rDNA directed polymerase chain reaction, and checkerboard DNA-DNA hybridization for detection of Fusobacterium nucleatum in endodontic infections. J Endod. 2002;28:86–9.
Myers GL, Montgomery S. A comparison of weights of debris extruded apically by conventional filing and Canal Master techniques. J Endod. 1991;17:275–9.
Ruff CM, Kriewaldt LM, Webb CT. New advances in nickel-titanium rotary systems. Clinic Update. 2013;35:1–8.
Seltzer S, Naidorf IJ. Flare-ups in endodontics: I. Etiological factors. J Endod. 1985;11:472–8.
Shovelton DS. The presence and distribution of microorganisms within non-vital teeth. Br Dent J. 1964;117:101–7.
Sundqvist G, Fidgor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:86–93.
Sundqvist G. Taxonomy, ecology, and pathogenicity of the root canal flora. Oral Surg Oral Med Oral Pathol. 1994;78:522–30.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Get vaccinated when it is your turn and follow the local guidelines. International Journal of Health Sciences, 5(3), x-xv. https://doi.org/10.53730/ijhs.v5n3.2938
Tanalp J, Güngör T. Apical extrusion of debris: A literature review of an inherent occurrence during root canal treatment. Int Endod J. 2014;47:211–21.
Tanalp J, Kaptan F, Sert S, Kayahan B, Bayirl G. Quantitative evaluation of the amount of apically extruded debris using 3 different rotary instrumentation systems. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:250–7. [PubMed] [Google Scholar]
Tinaz AC, Alacam T, Uzun O, Maden M, Kayaoglu G. The effect of disruption of apical constriction on periapical extrusion. J Endod. 2005;31:533–5.
Vande Visse JE, Brilliant JD. Effect of irrigation on the production of extruded material at the root apex during instrumentation. J Endod. 1975;1:243–6.
Zan R, Topcuoglu HS, Tunc T, Ozer SG, Hubbezoglu I, Kutlu G. Apical extrusion of intracanal biofilm after root canal preparation using Revo-S, Twisted File Adaptive, One Shape New Generation, ProTaper Next, and K3XF instrumentation systems. Turk Endod J. 2016;1:73–9.
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