An in vitro study of apical bacterial extrusion after root canal instrumentation using several endodontic file systems

Neel Vipul Doshi; Priyank Saxena; Aastha  Shukla; Anamika Sinha; Hrishikesh Ashok Saojii; Sheetal Mali

doi:10.53730/ijhs.v6nS5.11141

Authors

Neel Vipul Doshi
neeldoshi1529@gmail.com
MDS - Department of operative and conservative dentistry, (Ukrainian medical and stomatological university, Ukraine)
Priyank Saxena Junior Resident, Department of Dentistry, Atal Bihari Vajpayee Government Medical College, Vidisha, Madhya Pradesh
Aastha Shukla Assistant professor, Department of Conservative Dentistry and Endodontics, Modern Dental College, Indore
Anamika Sinha Assistant professor, Department of Conservative Dentistry and Endodontics, Bharati Vidyapeeth (Deemed to be University) Dental college and Hospital, Navi Mumbai
Hrishikesh Ashok Saojii Assistant professor, Department of Conservative Dentistry and Endodontics, Bharati Vidyapeeth (Deemed to be University) Dental college and Hospital, Navi Mumbai
Sheetal Mali Reader, Department of Conservative Dentistry and Endodontics, Bharati Vidyapeeth (Deemed to be University) Dental college and Hospital, Navi, Mumbai

Keywords:

Fusobacterium nucleatum, edge taper platinum, Hyflex CM, K3X

Abstract

Aim: In this research, the amount of apical extrusion of bacteria will be measured utilizing root canal instruments, including the K3XF, Protaper Gold, Edge taper Platinum, and Hyflex CM Rotary devices. Materials and Procedures: The saline solution contained sixty recently removed maxillary incisor teeth. After the trenches had been dug, the mash and bacteria were removed and placed in the entry pit to be used. In order to facilitate the gathering of germs, the teeth were attached to a device. Fusobacterium Nucleatum (ATCC 25586) was used to taint and then dry the root trenches for 24 hours. Six meetings made use of various tape systems, including K3XF edge tighten platinum and Hyflex CM turning reports, while the first four made use of hand-written K-records. Group 1 lacked any instrumentation (the control pack). The number of state molding units was tallied after 24 hours of Mueller-Hinton agar testing. The Kruskal-Wallis and Mann-Whitney U tests were used to analyse the data individually. Results: The K3XF file system emitted the fewest germs as compared to the other four rotating systems. Conclusion: Bacteria from intracanal spaces were ejected apically by all instrumentation methods.

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