Comparison of antimicrobial efficacy of chemical mouthwash and herbal mouthwash
An ex vivo study on orthodontic arch wires
Keywords:
chlorhexidine gluconate, cinnamomum zeylanicum, herbal, oral hygieneAbstract
Background: The use of mouth rinse for oral hygiene maintenance has been advocated as an adjuvant to mechanical aids of plaque removal. The aim of this study was to compare the antimicrobial effect of herbal and chemical mouth rinse on bacterial accumulation on orthodontic arch wires. Materials and Methods: The study sample consisted of 15 numbers of 0.016 Nickel Titanium (NiTi) arch wires removed from patients' mouths, which were split into three segments of 1 cm each. Wires were dipped in solutions of Chlorhexidine (Group A), Befresh Herbal (Group B) and Distilled water (Group C) individually for 30 seconds and then in normal saline for one minute. 0.5microlitre of this saline solution was inoculated in a Brain heart infusion medium (BHI) for 24 hours and then examined for bacterial count. The obtained results were subjected to One-way ANOVA, Bonferroni and post hoc tests for statistical analysis. Results: Group A showed the maximum reduction in the bacterial colony forming units (CFU) after 24 hours with a mean value of 65.67cfu. Group B showed a mean value of 336.87cfu. There was a statistically significant difference between the Chlorhexidine group and the Control group.
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Addy M, Slayne MA, Wade WG. The formation and control of dental plaque-an overview.Journal of Applied Bacteriology. 1992.73:269–78.
Baehni PC, Takeuchi Y. Anti-plaque agents in the prevention of biofilm-associated oral diseases. Oral Diseases. 2003.9:23–9.
Badawi H, Evans RD, Wilson M, Ready D, Noar JH, Pratten J. The effect of orthodontic bonding materials on dental plaque accumulation and composition in vitro. Biomaterials. 2003. 24(19):3345–50.
Øgaard B. White Spot Lesions During Orthodontic Treatment: Mechanisms and Fluoride Preventive Aspects. Seminars in Orthodontics. 2008. 14:183–93.
Zachrisson BU, Zachrisson S. Gingival Condition Associated with Partial Orthodontic Treatment. Acta Odontologica Scandinavica. 1972. 30:127–36.
Jones CG. Chlorhexidine: is it still the gold standard? Periodontology 2000. 1997.15:55–62.
Zanatta FB, Antoniazzi RP, Rösing CK. Staining and calculus formation after 0.12% chlorhexidine rinses in plaque-free and plaque covered surfaces: a randomized trial. J Appl Oral Sci. 2010. 18(5):515–21.
Flötra L, Gjermo P, Rölla G, Waerhaug J. Side effects of chlorhexidine mouth washes. European Journal of Oral Sciences. 1971. 79:119–25.
Danaei SM, Safavi A, Roeinpeikar SMM, Oshagh M, Iranpour S, Omidkhoda M. Ion release from orthodontic brackets in 3 mouthwashes: an in-vitro study. Am J Orthod Dentofacial Orthop. 2011. 139(6):730–4.
Husain S. Influence of Commercially Available Herbal Mouthwash on the Surface Tomography of Two Different Types of Nickel Titanium Orthodontic Arch Wires - An in vitro Study. Bioscience Biotechnology Research Communications. 2020. 13:1331–5.
Kripal K. Evaluation of a Herbal Mouthwash (BefreshTM) Vs. Chlorhexidine Mouthwash (Clohex Plus): A Prospective Clinical and Microbiological Study. 2017
Thakur S, Malagi S, Acharya AB. Evaluation of the Antimicrobial and Anti-inflammatory Efficacy of Two Commercially Available Mouthwashes. Journal of Contemporary Dentistry. 2017. 7:119–21.
Jain A, Lavate A, Yadav P, Agali C, Bhaskar D, Chaturvedi M, et al. Comparative evaluation of honey, chlorhexidine gluconate (0.2%) and combination of xylitol and chlorhexidine mouthwash (0.2%) on the clinical level of dental plaque: A 30 days randomized control trial. Perspectives in Clinical Research. 2015. 6:53.
Joshi V, Pathan M, Bhat K. Comparative evaluation of the efficacy of a herbal mouthwash and chlorhexidine mouthwash on select periodontal pathogens: An in vitro and ex vivo study. Journal of Indian Society of Periodontology. 2018.
Matan N, Rimkeeree H, Mawson AJ, Chompreeda P, Haruthaithanasan V, Parker M. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Int J Food Microbiol. 2006. 15;107(2):180–5.
Wiwattanarattanabut K, Choonharuangdej S, Srithavaj T. In Vitro Anti-Cariogenic Plaque Effects of Essential Oils Extracted from Culinary Herbs. J Clin Diagn Res. 2017. 11(9):DC30–5.
Fischman SL. The history of oral hygiene products: how far have we come in 6000 years? Periodontol 2000. 1997. 15:7–14.
Nayak SU, Kumari A, Rajendran V, Singh VP, Hegde A, Pai KK. Comparative Evaluation of Efficacy of Chlorhexidine and Herbal Mouthwash as a Preprocedural Rinse in Reducing Dental Aerosols: A Microbiological Study. International Journal of Dentistry. 2020. 1–6.
Anjum A, Hosein M, Butt SA, . F, Fawad B, Abidi F. Efficacy of Two Mouth Rinses in Reducing Aerosol Bacterial Load during Ultrasonic Scaling. Journal of Advances in Medicine and Medical Research. 2019. 1–9.
Ambati M, Pinnamaneni I, Prasanna J, Rajashree D, Rani K, Reddy P. Chemical vs. herbal formulations as pre-procedural mouth rinses to combat aerosol production: A randomized controlled study.Journal of Oral Research and Review. 2014. 6:9.
Gupta D, Nayan S, Tippanawar HK, Patil GI, Jain A, Momin RK, et al. Are herbal mouthwash efficacious over chlorhexidine on the dental plaque? Pharmacognosy Res. 2015. 7(3):277–81.
Bay NL, Overman PR, Krust-Bray K, Cobb C, Gross KB. Effectiveness of antimicrobial mouthrinses on aerosols produced by an air polisher. J Dent Hyg. 1993. 67(6):312–7.
Nagappan N, John J, Gopinath NM, Elango SK, Pillai DDM, Mani M. Antimicrobial Effectiveness of Herbal and 0.2% Chlorhexidine Mouthrinse against Streptococcus mutans: An In-vitro Study. Journal of International Oral Health. 2016. 1;8(6):683.
Parwani S, Parwani R, Chitnis PJ, Dadlani H, Prasad SS. Comparative evaluation of anti-plaque efficacy of herbal and 0.2% chlorhexidine gluconate mouthwash in a 4-day plaque re-growth study. Journal of Indian Society of Periodontology. 2013. 17:72.
Manipal S, Hussain S, Wadgave U, Duraiswamy P, Ravi K. The Mouthwash War - Chlorhexidine vs. Herbal Mouth Rinses: A Meta-Analysis. J Clin Diagn Res. 2016. 10(5):ZC81–3.
Dolles OK, Eriksen HM, Gjermo P. Tooth stain during 2 years’ use of chlorhexidine- and fluoride-containing. European Journal of Oral Sciences. 1979. 87:268–74.
Schiff N, Grosgogeat B, Lissac M, Dalard F. Influence of fluoridated mouthwashes on corrosion resistance of orthodontics wires. Biomaterials. 2004. 25:4535–42.
Eslami F, Aghili H, Yassaei S. Evaluation of the effect of three mouthwashes on the mechanical properties and surface morphology of several orthodontic wires: An in vitro study. Dental Research Journal. 2017. 14:252.
Singh J, Gautam A, Joshi A, Manjooran T, Raghav S, Patel JH. An in vitro Evaluation of Shear Bond Strength of Orthodontic Brackets after Mouth Rinse. The Journal of Contemporary Dental Practice. 2018. 16:862–6.
Cai H, Chen J, Panagodage Perera NK, Liang X. Effects of Herbal Mouthwashes on Plaque and Inflammation Control for Patients with Gingivitis: A Systematic Review and Meta-Analysis of Randomised Controlled Trials. Evid Based Complement Alternat Med. 2020. 20;2020:2829854.
Gujjari S. Cinnamon Mouthwash as an Anti-Fungal Agent in Treatment of Diabetic Patients with Chronic Periodontitis. Advances in Dentistry & Oral Health. 2018.
Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology. 2004. 94:223–53.
Kwon JA, Yu CB, Park HD. Bacteriocidal effects and inhibition of cell separation of cinnamic aldehyde on Bacillus cereus. Lett Appl Microbiol. 2003;37(1):61–5.
Gupta A, Kumar R, Kumar H, Garg H. Comparative performance of pure vegetable oil and Al2O3 based vegetable oil during MQL turning of AISI 4130. Materials Today: Proceedings. 2020. 28:1662–6.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
Gandamayu, I. B. M., Antari, N. W. S., & Strisanti, I. A. S. (2022). The level of community compliance in implementing health protocols to prevent the spread of COVID-19. International Journal of Health & Medical Sciences, 5(2), 177-182. https://doi.org/10.21744/ijhms.v5n2.1897
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