Decreasing TNFα in serum and increasing mitotic index rate in albino mice treated with melatonin

Marwa Majeed Hameed; Muthana Ibrahim Maleek

doi:10.53730/ijhs.v6nS4.12106

Authors

Marwa Majeed Hameed
majedmarwa023@gmail.com
Department of Biology, College of Science, University of Wasit, Iraq
Muthana Ibrahim Maleek Department of Biology, College of Science, University of Wasit, Iraq

Keywords:

melatonin, TNF α, mitotic index, albino mice

Abstract

Melatonin, as an endogenous hormone, is associated with the moderation of the circadian rhythm. Besides controlling the sleep-wake cycle, many physiological functions of melatonin have been identified, such as antioxidant, immune modulating, and anti-inflammatory. The current study was designed to estimate the effect of different doses of melatonin on gene expression of (TNF) in serum and mitotic index (MI) in bone marrow stem cells as cytogenetic test. This study was conducted at Wasit University, College of Science, Department of Biology, Cytogenetic Laboratory, from the period 2/11/2021 to 14/4/2022 using albino mice. The mice were divided into four groups, a control group and three groups divided according to melatonin concentration (5, 10, &15 mg/kg), and were treated for seven consecutive days for every dose. Results showed a significant decrease in gene expression (protein) of TNF α- at P≤0.05 for all three doses of melatonin that were used and mitotic index numbers increased at P≤0.05 for the same doses above of melatonin, when all results above were compared with the control group.

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References

Allen, J. W., Shuler, C. F., Mendes, R. W., & Latt, S. A. (1977). A simplified technique for in vivo analysis of sister-chromatid exchanges using 5-bromodeoxyuridine tablets. Cytogenetic and Genome Research, 18(4), 231-237.‏

Al-Mothafar, N. A., Al-Shahwany, A. W., & Salih, S. J. (2022). Comparing the histological effect of Thymol from multiple sources with blood lipids profile and liver enzymes in Albino Mice. International Journal of Health Sciences, 6(S1), 244–256. https://doi.org/10.53730/ijhs.v6nS1.4770

Alonso-González, C., González, A., Menéndez-Menéndez, J., Martínez-Campa, C., & Cos, S. (2020). Melatonin as a radio-sensitizer in cancer. Biomedicines, 8(8), 247.‏

Atretkhany, K. S. N., Gogoleva, V. S., Drutskaya, M. S., & Nedospasov, S. A. (2020). Distinct modes of TNF signaling through its two receptors in health and disease. Journal of leukocyte biology, 107(6), 893-905.‏

Beljaards, R. C., Kaudewitz, P., Berti, E., Gianotti, R., Neumann, C., Rosso, R., ... & Willemze, R. (1993). Primary cutaneous CD30‐positive large cell lymphoma: definition of a new type of cutaneous lymphoma with a favorable prognosis. A European Multicenter Study of 47 patients. Cancer, 71(6), 2097-2104

Bromodeoxyuridine tablets with improved depot effect for analysis in vivo of sister-chromatid exchanges in bone-marrow and spermatogonial cells. Mutation Research/Environmental Mutagenesis and Related Subjects, 97(2), 117-129.‏

Chen, Y., Ning, J., Cao, W., Wang, S., Du, T., Jiang, J., ... & Zhang, B. (2020). Research progress of TXNIP as a tumor suppressor gene participating in the metabolic reprogramming and oxidative stress of cancer cells in various cancers. Frontiers in oncology, 10, 568574.

Coussens, L. M., & Werb, Z. (2002). Inflammation and cancer. Nature, 420(6917), 860-867.

Duijf, P. H., Schultz, N., & Benezra, R. (2013). Cancer cells preferentially lose small chromosomes. International journal of cancer, 132(10), 2316-2326.

Ferreira, G. M., Martinez, M., Camargo, I. C. C., Domeniconi, R. F., Martinez, F. E., & Chuffa, L. G. A. (2014). Melatonin attenuates Her-2, p38 MAPK, p-AKT, and mTOR levels in ovarian carcinoma of ethanol-preferring rats. Journal of Cancer, 5(9), 728.

Fitzgibbons, P. L., Page, D. L., Weaver, D., Thor, A. D., Allred, D. C., Clark, G. M., ... & Schnitt, S. J. (2000). Prognostic factors in breast cancer: College of American Pathologists consensus statement 1999. Archives of pathology & laboratory medicine, 124(7), 966-978.‏

Gamez, M. R., Perez, A. V., Falcones, V. A. M., & Bazurto, J. J. B. (2019). The geoportal as strategy for sustainable development. International Journal of Physical Sciences and Engineering, 3(1), 10–21. https://doi.org/10.29332/ijpse.v3n1.239

Hardeland, R. (2018). Melatonin and inflammation—Story of a double‐edged blade. Journal of pineal research, 65(4), e12525

Ismeel, A. O. (2011). Cytogenetic and cytotoxic studies on the effect of phytoinvestigated active compounds of Hyoscyamus niger (in vivo and ex vivo) (Doctoral dissertation, Ph. D. Thesis in Philosophy of Sciencein Biotechnology, University of Al-Nahrain, Iraq).‏

King, M. T., Wild, D., Gocke, E., & Eckhardt, K. (1982).

Leibowitz, M. L., Zhang, C. Z., & Pellman, D. (2015). Chromothripsis: a new mechanism for rapid karyotype evolution. Annual review of genetics, 49, 183-211.

Liu, X., Xu, Y., Chen, S., Tan, Z., Xiong, K., Li, Y., & Gong, Y. (2014). Rescue of proinflammatory cytokine-inhibited chondrogenesis by the antiarthritic effect of melatonin in synovium mesenchymal stem cells via suppression of reactive oxygen species and matrix metalloproteinases. Free Radical Biology and Medicine, 68, 234-246.

Maleek M I, Faraj S A and Alzubaidi D A (2016) Cytogenetic Effects of Ranitidine on Stem Cell L-Asparaginase Treated Mice. Int. J.

Naylor, R. M., & Van Deursen, J. M. (2016). Aneuploidy in cancer and aging. Annual review of genetics, 50, 45.

Qiu, X., Wang, X., Qiu, J., Zhu, Y., Liang, T., Gao, B., ... & Huang, D. (2019). Melatonin rescued reactive oxygen species-impaired osteogenesis of human bone marrow mesenchymal stem cells in the presence of tumor necrosis factor-alpha. Stem cells international, 2019.

Reiter, R. J., Mayo, J. C., Tan, D. X., Sainz, R. M., Alatorre‐Jimenez, M., & Qin, L. (2016). Melatonin as an antioxidant: under promises but over delivers. Journal of pineal research, 61(3), 253-278.‏

Rodrigues, M. A., Beaton-Green, L. A., Wilkins, R. C., & Fenech, M. F. (2018). The potential for complete automated scoring of the cytokinesis block micronucleus cytome assay using imaging flow cytometry. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 836, 53-64.‏

Salomon, B. L., Leclerc, M., Tosello, J., Ronin, E., Piaggio, E., & Cohen, J. L. (2018). Tumor necrosis factor α and regulatory T cells in oncoimmunology. Frontiers in immunology, 9, 444.‏

Sci. & Engineering Investigations 5, 45-51

Shubber, E. K., & Al-Allak, B. M. A. (1986). Spontaneous chromosomal aberrations and SCEs in human lymphocytes. Effects of culture conditions. Nucleus, 29, 92-8.‏

Sumsuzzman, D. M., Khan, Z. A., Choi, J., & Hong, Y. (2021). Differential role of melatonin in healthy brain aging: A systematic review and meta-analysis of the SAMP8 model. Aging (Albany NY), 13(7), 9373.

Varfolomeev, E., & Vucic, D. (2018). Intracellular regulation of TNF activity in health and disease. Cytokine, 101, 26-32.

Vinther, A. G., & Claesson, M. H. (2015). The influence of melatonin on immune system and cancer. Int. J. Cancer Clin. Res, 2(4).

Widana, I.K., Sumetri, N.W., Sutapa, I.K., Suryasa, W. (2021). Anthropometric measures for better cardiovascular and musculoskeletal health. Computer Applications in Engineering Education, 29(3), 550–561. https://doi.org/10.1002/cae.22202

Zhang, C. Z., Spektor, A., Cornils, H., Francis, J. M., Jackson, E. K., Liu, S., ... & Pellman, D. (2015). Chromothripsis from DNA damage in micronuclei. Nature, 522(7555), 179-184.‏

Zhang, Y. L., & Dong, C. (2005). MAP kinases in immune responses. Cell Mol Immunol, 2(1), 20-27.

Zisapel, N. (2018). New perspectives on the role of melatonin in human sleep, circadian rhythms and their regulation. British journal of pharmacology, 175(16), 3190-3199.