Reconsiderations with basic viral tests in Wasit blood bank: CMV and EBV as an examples
Keywords:
Cytomegalovirus, Real-Time PCR, Enzyme –linked immuno sorbent assay, Polymerase chain reaction, Epstein-Barr virusAbstract
Background: Blood donation is a procedure of collecting blood from a screened person who must follow the strict guidelines in the country where peoples lived. Aim of the study: To increase the safety of blood given to patients by evaluating the addition of new investigations to detect CMV and EBV infection of donated blood in the blood bank in Wasit Governorate. Patients and Methods: This study included 150 samples of blood taken from blood bags of donors from blood bank /Wasit Province. In this study used ELISA used only for detect infection with CMV and Real-Time PCR were used to detect infections with CMV and EBV. Results: The results of ELISA from 150 analyzed samples only 30/150 (20%) of the patients were diagnosed infection by CMV. While the results of RT-PCR 50/150 (33.3%) for the same virus, and the results of RT-PCR 13/150 (8.7%) for EBV. Conclusion: The guideline of blood and blood product donation should be reviewed to keep up the updated guideline of WHO to provide more suitable medical services to community.
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