Electrocardiographically study of protective effect of Eruca Sativa M. versus doxorubicin induced cardiotoxicity in rabbits

Zuhair Abdulkareem Dawah; Rana Abdulla Salih

doi:10.53730/ijhs.v6nS6.12182

Authors

Zuhair Abdulkareem Dawah
zouher.kareem1206h@covm.uobaghdad.edu.iq
Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Rana Abdulla Salih Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq

Keywords:

eruca sativa M, doxorubicin, ECG, cardiotoxicity, rabbit, Iraq

Abstract

Cardiotoxicity is defined as drug-induced damage to the heart muscle, which can result in cardiac failure or cardiac arrest. Cardiotoxicity is a common side effect of traditional anticancer drugs such as doxorubicin, taxanes, 5-fluorouracil, and cyclophosphamide, as well as newer agents such as biological antibodies such as Nivo. Cardiotoxicity is also caused by the long-term use of neurologic/psychiatric medications. This study was designed to determine the effect of eruca sativa mill (Gergeer) leaves extract on cardiotoxicity induced by doxorubicin in rabbits. The thirty-five rabbits used in this study were obtained from a local rabbit breeder. Animals ranged between 1-1.3 years old, and their weight was approximately 1-2kg without specifying gender. experiment: Thirty-five adult rabbits (n=35) will be randomly divided into four groups (7 rabbits/each group) first group: seven rabbits will receive distal water (D.W) orally (-ve control), Second group: fourteen rabbits divided into two subgroup equally administrated with doxorubicin 4 mg/kg B.W (I.P) between day to day for 14 days to induce cardiotoxicity (the first subgroup leave two weeks then killed, while the second subgroup leaves to 28 days without treatment),

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References

Abd-Almoneim, S. S., & Mahmood, A. K. (2019). Survey on heart disorder in dogs by electrocardiography in Iraq. Biochemical and Cellular Archives, 19(2), 3353-3361.‏

Akgül, C., Akcicek, A., Karadağ, A., & Karasu, S. (2022). Formulation optimization of low-fat emulsion stabilized by rocket seed (Eruca Sativa Mill) gum as novel natural fat replacer: effect on steady, dynamic and thixotropic behavior. Acta Scientiarum. Technology, 44, e56006-e56006

AL-OKaily,B.N.; AL-Haddad,A.A and Salman,A.D.(2013). Effect of Different Doses of Doxorubicin on pituitary Gland and some Testicular function in adults Male Rabbits. The Iraqi Journal of Veterinary medicine, 37C1):121-128

Awadelkareem, A. M., Al-Shammari, E., Elkhalifa, A. E. O., Adnan, M., Siddiqui, A. J., Snoussi, M., ... & Ashraf, S. A. (2022). Phytochemical and In Silico ADME/Tox Analysis of Eruca sativa Extract with Antioxidant, Antibacterial and Anticancer Potential against Caco-2 and HCT-116 Colorectal Carcinoma Cell Lines. Molecules, 27(4), 1409.‏

Baxter, S. ;Blackman, S. ;Breen, C. ;Brown, C. ;Campbell, B. ;Cox, C. ;Eldridge, J. ;Hutchinson, J. ;Rees, E. ;Richley, D. and Ross, C. (2014). Clinical Guidelines by Consensus. Recording a standard 12- lead electrocardiogram. An approved methodology by society for cardiological science and technology (SCST). Ulster University, Northern Ireland, P. 5-25.

Baxter, S.; Blackman, S.; Breen, C.: Brown, C.: Campbell, B.; Cox, C.; Eldridge, J. Belizna, C.C., Richard, V., Thuillez, C., Levesque, H. and Shoenfeld, Y. (2007). Insights into Atherosclerosis Therapy in Antiphospholipid Syndrome. Autoimmun Rev, 7: 46-51.

Bornstein, A. B., Rao, S. S., & Marwaha, K. (2021). Left Ventricular Hypertrophy. In StatPearls [Internet]. StatPearls Publishing.‏

Ceccanti, C., Landi, M., Benvenuti, S., Pardossi, A., & Guidi, L. (2018). Mediterranean wild edible plants: Weeds or “new functional crops”?. Molecules, 23(9), 2299.‏

Durlacher, C. T., Chow, K., Chen, X. W., He, Z. X., Zhang, X., Yang, T., & Zhou, S. F. (2015). Targeting Na+/K+‐translocating adenosine triphosphatase in cancer treatment. Clinical and Experimental Pharmacology and Physiology, 42(5), 427-443.‏

Erwin Christian, de Bliek (2018). "ST elevation: Differential diagnosis and caveats. A comprehensive review to help distinguish ST elevation myocardial infarction from nonischemic etiologies of ST elevation". Turkish Journal of Emergency Medicine.

Gandamayu, I. B. M., Antari, N. W. S., & Strisanti, I. A. S. (2022). The level of community compliance in implementing health protocols to prevent the spread of COVID-19. International Journal of Health & Medical Sciences, 5(2), 177-182. https://doi.org/10.21744/ijhms.v5n2.1897

Iqubal,A. ; Haque,S.Eh. ; sharma,S. ; Ansari, M.A. ; khan, V and Iqubal, M.K.(2018).Clinical updates on Drug Induced cardiotoxicity. IJPSR , 9(1):16-26

Johnson-Arbor, K., and Dubey, R. (2017). Doxorubicin.

Kancharlapalli, S., Sweeya, P. S., Gandham, A. P. D., and Rao, V. U. M. (2014). CARDIOTOXICITY AND USE OF HERBAL DRUGS- A REVIEW. Asian Journal of Pharmaceutical Research and Development, 2(1), 1–9.

Kautzner J, Malik M. (1997) QT interval dispersion and its clinical utility. Pacing and Clinical Electrophysiology. Oct;20(10):2625-40.

Klabunde, R.E. (2013). Cardiovascular physiology concepts. Electrocardiogram leads.

Kumar, S. (2007). ECG tutorial for clinicians. Basic competency in electrocardiography.

Lippincott Williams & Wilkins (2009). ECG Facts Made Incredibly Quick!. Second Edition. Printed in China. p. 5-6.

Lynch, R. (2014). ECG lead misplacement: A brief review of limb lead misplacement. African Journal of Emergency Medicine, 4: 130–139.

Maglaveras, N. ;Stamkopoulos , T. ;Diamantaras, K. ;Pappas , C. and Strintzis, M.(1998). ECG pattern recognition and classification using non-linear transformations and neural networks. International Journal of Medical Informatics, 52 (1-3):191-209.

Nakamura, K., Miyoshi, T., Yoshida, M., Akagi, S., Saito, Y., Ejiri, K., ... & Ito, H. (2022). Pathophysiology and Treatment of Diabetic Cardiomyopathy and Heart Failure in Patients with Diabetes Mellitus. International journal of molecular sciences, 23(7), 3587

Peters, A. ;Liu, E. and Verrier, R.L. (2000). Air pollution and incidence of cardiac arrhythmia. Epidemiology, 11(1): 11-17.

Rautaharju, P. M., Surawicz, B., & Gettes, L. S. (2009). AHA/ACCF/HRS recommendations for the standardization and interpretation of the electrocardiogram: part IV: the ST segment, T and U waves, and the QT interval a scientific statement from the American Heart Association Electrocardiography and Arrhythmias Committee, Council on Clinical Cardiology; the American College of Cardiology Foundation; and the Heart Rhythm Society Endorsed by the International Society for Computerized Electrocardiology. Journal of the American College of Cardiology, 53(11), 982-991.‏

Rezakhani, A. and Rezaian, G. (1995). Clinical electrocardiogram of laboratory white New Zealand rabbits, Journal of Applied Animal Research, 7(1): 63-68.

Rockley, K. (2018). In Vitro Evaluation of Anthracycline-induced Cardiotoxicity and Mitigation by Perturbation of Angiotensin Signalling (Doctoral dissertation, Durham University).

Salih, R. A. (2017). Toxic effects of subacute exposure to Nerium oleander leaves hexane extract on the heart of rabbits: Ali Aziz Alkhayyat and Rana Abdulla Salih. The Iraqi Journal of Veterinary Medicine, 41(2), 54-60.‏

Salih, R. A., & Alkhayyat, A. A. (2016). Toxic effect of Nerium Oleander leaves extract on biochemical parameters in rabbit serum. Al-Anbar J. Vet. Sci, 9(2), 1-8.‏

Shaker, R. A., Abboud, S. H., Assad, H. C., and Hadi, N. (2018). Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis. BMC Pharmacology and Toxicology, 19(1), 1-10.

Sur M, Mohiuddin SS. (2021) In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; Jan-.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Swamy, V. A. H. M., Wangikar, U., Koti, B. C., Thippeswamy, A. H. ., Ronad, P. M., and Manjula, D. V. (2011). Cardioprotective effect of ascorbic acid on doxorubicin-induced myocardial toxicity in rats. Indian Journal of Pharmacology, 43(5), 507–511.

Taleb, A., Ahmad, K. A., Ihsan, A. U., Qu, J., Lin, N., Hezam, K., Koju, N., Hui, L., and Qilong, D. (2018). Antioxidant effects and mechanism of silymarin in oxidative stress induced cardiovascular diseases. Biomedicine and Pharmacotherapy, 102, 689–698.

Turhan, A. S., Can, B. G., Kabay, T., & Şensoy, S. (2022). The Effect of Use of Microalgae [Chlorella vulgaris Beyerinck (Beijerinck)] in Different Fertilizer Applications on Plant Growth of Garden Rocket (Eruca vesicaria ssp. sativa Mill.). Turkish Journal of Agriculture-Food Science and Technology, 10(2), 323-329.‏

Watanabe, RenatoHyperkalemia in chronic kidney disease. Revista da Associação Médica Brasileira . 2020, v. 66, n. Suppl 1 [Accessed 22 June 2021] , pp. s31-s36.

Weli, S. M., Kareem, S. I., Shareef, O. H., Rauf, H. G., Raouf, C. G., & Hama, A. A. (2021). The effects of the aqueous extract of the leaves of the local plant eruca sativa on lipid profile and some minerals in the blood of male white mice. Iraqi Journal of Science, 62(4), 1088–1093.

Wiegerinck RF, Verkerk AO, Belterman CN, van Veen TA, Baartscheer A, Opthof T,

Wilders R, de Bakker JM, Coronel R. (2006) Larger cell size in rabbits with heart failure increases myocardial conduction velocity and QRS duration. Circulation. Feb 14;113(6):806-13.