The bioactive role of aqueous extract of aegle marmelos leaves on obese rats

Asmaa M. Sharshar; Ahmed A. Ameen; Hany G. El-Masry

doi:10.53730/ijhs.v6nS9.12260

Authors

Asmaa M. Sharshar Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
Ahmed A. Ameen Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
Hany G. El-Masry Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt

Keywords:

Aegle marmelos, High fat diet, Obesity, Rats

Abstract

The aim of this study was to evaluate the effect of Aegle marmelos aqueous extract (AMAE) leaves on obese rats. Twenty-five adult male albino rats (Sprague-Dawley strain), weighing about (200±10g) were divided randomly into two main groups as follow: the first group (-Ve control= 5 rats) was fed on basal diet. The second group (20 rats) was fed on high fat diet (HFD) for 4 weeks then divided equally to 4 groups from group 2 to group 5. Group 2 (+Ve control) fed on basal diet. Group 3, 4 and 5 fed on HDF administrated orally with 1, 2 and 3 ml of AMAE, respectively. At the end of the experimental period (4 weeks), rats were scarified and serum was collected to determine levels of lipid profile, glucose, leptin kidneys and liver functions. The results showed that the body weight of rats and serum levels of lipids profile, leptin, glucose, kidneys functions and liver enzymes were elevated by HFD administration (positive control group) compared with negative control group, while HDL-C decreased. It was also found that oral administration of AMAE diet with reversed these changes that caused by HFD.

Downloads

Download data is not yet available.

References

Ahmed, O., Hozayen, W., Bastawy, M. and Hamed, M., (2011): Biochemical effects of cichorium intybus and sonchus oleraceus infusions and esculetin on streptozotocin-induced diabetic albino rats. Journal of American Science 7: 1124-1137.

Albers, N., Benderson V. and Warnick G. (1983): Enzaymatic determination of high-density lipoprotein cholesterol, Selected Methods. Clin. Chem., 10:91-99.

Armitage, G.Y. and Berry, W.G. (1987): Statistical methods 7th Ed. Ames., Iowa State University. Press. 39-63.

Bautista, R., Mahmoudb, A., Konigsberga, M. and Guerrero, N. (2019): Obesity: pathophysiology, monosodium glutamate-induced model and antiobesity medicinal plants, Biomed. Pharmacothe, 111: 503-516,

Bhuvaneswari, R. and Sasikumar, K. (2013): Antihyperlipidemic activity of Aegle marmelos (L) Corr., leaf extract in triton wr- 1339 induced hyperlipidemic rats. Pharmacie Globale, 4(3): 1.

Carbone, S., Del Buono, M., Ozemek, C. and Lavie, C. (2019): Obesity, risk of diabetes and role of physical activity, exercise training and cardiorespiratory fitness, Prog. Cardiovasc. Dis., 62: 327-333.

Chandel, S., Patel, P., Janghel, V. and Sahu, R. (2020): Dietary Inclusion of Aegle Marmelos Fruits in Wistar Rats. eIJPPR., 10(3):3-12.

Chapman D. G., Gastilla R. and Campbell J. A. (1959): Evaluation of protein in foods: 1- A Method for the determination of protein efficiency ratio. Can. J. Biochem. Phys; 37:679- 686.

Chockalingam, V., Kadali, S. and Gnanasambantham, P. (2012): Antiproliferative and antioxi¬dant activity of Aegle marmelos (Linn.) leaves in Dalton’s Lymphoma Ascites transplanted mice. Indian J Pharmacol., 44(2): 225-229.

Corpeli, J., Bakker, S. and Stoic, R. (2009): Obesity and impaired renal function potential for life style intervention. Eur J Epidemiol, 24(6): 275-280.

Fernandez-Navarro, T., Díaz, I., Guti´errez-Díaz, I., Rodríguez-Carrio, J., Su´ arez, A. and Reyes-Gavilan, C. (2019): Exploring the interactions between serum free fatty acids and fecal microbiota in obesity through a machine learning algorithm, Food Res. Int. 121: 533-541,

Fridewald, W., Leve, R. and Fredrickson, D. (1972): Estimation of the concentration of low-density lipoprotein separated by three different methods. Clin. Chem., 18: 499-502.

Garg, A. and Singh, R. (2015): Antiobesity activity of aqueous and ethanol extracts of Aegle marmelos leaves in high fat diet induced obese rats. Int. J. Pharm. Sci. Rev. Res., 30(1): 53-60.

Garg, A. and Singh, R. (2015): Antiobesity activity of aqueous and ethanol extracts of Aegle marmelos leaves in high fat diet induced obese rats. Int. J. Pharm. Sci. Rev. Res., 30(11): 53-60.

Ger, T., Fu, Y. and Chi, C. (2020): Bidirectional association between psoriasis and obstructive sleep apnea: a systematic review and meta-analysis, Sci. Rep.

Gorda, A. O. S., Romayanti, K. N., & Anggreswari, N. P. Y. (2018). Social capital, spiritual capital, human capital, and financial capital in the management of child welfare institutions. International Journal of Social Sciences and Humanities, 2(3), 12–20. https://doi.org/10.29332/ijssh.v2n3.183

Hadžović-Džuvo, A., Lepara, O., Valjevac, A., Avdagić, A. and Začiragić, A. (2014): Impact of high-fat diet on serum adiponectin and leptin level in streptozotocin-induced diabetes mellitus type 2. Folia Med. Fac. Med. Univ. Saraeviensis, 49(2): 110-115.

Herliah, A., Baso, Y. S., Hidayanty, H., Syarif, S., Aminuddin, A., & Bahar, B. (2022). Effect of web-based she smart education models on adolescent girl’s knowledge, attitudes, and practice about obesity. International Journal of Health & Medical Sciences, 5(1), 50-55. https://doi.org/10.21744/ijhms.v5n1.1832

Ibrahim, N. Mohammed, M. Aly, H. Ali, S. and Abd Al-Hadya, D. (2018): Eﬃciency of the leaves and fruits of Aegle marmelos methanol extract (L.) Correa and their relative hepatotoxicity induced by CCL4 and identiﬁcation of their active constituents by using LC/MS/MS. Toxicology Reports, 1161-1168.

Karmase, A., Birari, R. and Bhutani, K. (2013): Evaluation of anti-obesity effect of Aegle marmelos leaves. Phytomedicine, 20: 805-812.

Karmase, A., Birari, R. and Bhutani, K. (2013): Evaluation of anti-obesity effect of Aegle marmelos leaves. Phytomedicine 20: 805-812.

Kelley, D., Goodpaster, B. and Storlien L. (2002): Muscle triglyceride and insulin resistance, Annu Rev Nutr, 22: 325-346.

Kim, K., Kang, J., Jung, Y., Lee, S., Rametta, R., Dongiovanni, P., Valenti, L. and Pajvani, U. (2021): Adipocyte PHLPP2 inhibition prevents obesity-induced fatty liver, Nat. Commun., 12.

Kore, K. Shete, R. and Jadhav, P. (2011): Rp-Hplc Method of Simultaneous Nephroprotective Role f A. Marmelos Extract. IJRPC, 1(3).

Lasker, S., Rahman, M., Parvez, F., Zamila, M., Miah, P., Nahar, K., Kabir, F., Sharmin, S., Nusrat Subhan, N. and Ahsan, G. (2019): High-fat diet-induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation. Sci. Rep., 9:20026.

Malnick, S. and Knobler, H. (2006): The medical complications of obesity, QJM, 99: 65-79.

Maqbool, M., Gani, I. and Dar, M. (2019): Anti-Diabetic Effects of Some Medicinal Plants in Experimental Animals: A Review. Asian Journal of Pharmaceutical Research and Development, 7(1): 66-69.

Mehrzad, R. (2020): Definition and introduction to epidemiology of obesity, in: R. Mehrzad (Ed.), Obesity: Global Impact and Epidemiology, Elsevier, 1-6.

Michalovich, D., Rodriguez-Perez, N., Smolinska, S., Pirozynski, M., Mayhew, D. and Uddin, S. (2019): Obesity and disease severity magnify disturbed microbiomeimmune interactions in asthma patients, Nat. Commun.,

Milena, J. S. (2003): Predrag Djurdjevic and DejanStankov .JSCS. 68(8-9): 691-698.

Min, L., Ling, S., Yin, L., Stephen, C. W., Randy, J. David, D. and Patrick, T. (2004): Obesity induced by a high-fat diet downregulates apolipoprotein A-IV gene expression in rat hypothalamus. Am. J. Physiol. Endocrinol Metab. 287: 366-370.

Modi, H. Patel, V. and Patel, K. (2012): Hepatoprotective Activity of Aegle Marrmelos Against Ethanol Induced Hepatotoxicity in Rats. Asian J Pharm Clin Res, 5(4): 164-167.

NCD Risk Factor Collaboration, (2016): Trends in adult body-mass index in 200 countries from 1975 to 2014: a pooled analysis of 1698 population-based measurement studies with 192 million participants, Lancet, 387: 1377-1396.

Nurdiantami, Y., Watanabe, K., Tanaka, E., Pradono, J. and Anme, T. (2018): Association of general and central obesity with hypertension, Clin. Nutr., 37:1259-1263.

Polyzos, S., Kountouras, J. and Mantzoros, C. (2019): Obesity and nonalcoholic fatty liver disease: from pathophysiology to therapeutics, Metabolism, 92: 82-97.

Qiao, D., Li, Y., Liu, X., Zhang, X., Qian, X. and Zhang, H. (2020): Association of obesity with bone mineral density and osteoporosis in adults: a systematic review and meta-analysis, Public Health, 180: 22-28.

Ramesh, B. and Pugalendi, K. (2005): Antihyperlipidemic and antidiabetic effects of umbelliferone in streptozotocin diabetic rats. Yale Journal of Biology and Medicine 78: 189-196.

Ramesh, B., Viswanathan, P., Pugalendi,K. (2007): Protective effect of Umbelliferone on membranous fatty acid composition in streptozotocin-induced diabetic rats. European Journal of Pharmacology, 566: 231-239.

Reddy, P., Sahana, N and Urooj, A. (2012): activity of Aegle marmelos and Psidium guajava leaves. IJMAP., 2(1):155-160.

Reeves, P. Nielsen, F. and Fahmy, G. (1993): AIN-93. Purified diets for laboratory rodents: Final reports of the American Institute of Nutrition adhoe wriling committee of reformulation of the AIN-76 A Rodent Diet. J. Nutr., 123:1939-1951.

Richmond, N. (1973): Colorimetric determination of total cholesterol and high-density lipoprotein cholesterol (HDL-c). Clin. Chem., 19: 1350-1356.

Roy, S.E. (1970): colorimetric method of serum alkaline phosphatase. Journal of Clinical Chemistry, 16:431-432.

Sankeshi, V., Kumar, P., Naik, R., Sridhar, G., Kumar, M. and Gopal, V. (2013): Inhibition of aldose reductase by Aegle marmelos and its protective role in diabetic cataract. J Ethnopharmacol, 149(1): 215-221.

Siddiqui, T. Naz, L. Sohail, S. Yasmeen, G. Khan, N. and Shamim, M. (2019): Paracetamol induced Hepatotoxicity in Rats: Role of Aegle Marmelos (L.) Corrêa against Liver Damage. Int. J. Biol. Biotech., 16 (2): 313-317.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864

Trinder, P. (1969): Determination of blood glucose using 4- aminophenazone. J. Clin. Path., 22: 246.

Velasquez, M. and Bhathena, S. (2007): Role of dietary soy protein in obesity. Int J Med Sci., 4: 72-82.

Vijaya, C., Ramanathan, M. and Suresh, B. (2009): Lipid lowering activity of ethanolicextract of leaves of Aegle marmelos (Linn.) in hyperlipidaemic models of Wistaralbino rats. Indian Journal of Experimental Biology, 47: 182–185.

Vijaya. C. Ramanathan, M. and Suresh, B. (2009): Lipid lowering activity of ethanolic extact of leaves of Aegle marmelos (Linn.) in hyperlipidaemic model of Wistar albino rats. Indian J. Exp. Biol., 47: 182.

Wahlefeld, A. (1974): Methods of Enzymatic Analysis. Academic Press, Chapter, 5: 1831-1835.

Wang, K., Yu, X., Tang, Y., Tang, Y. and Liang, X. (2019): Obesity: an emerging driver of head and neck cancer, Life Sci.

Xia, J., Lloyd-Jones, D. and Khan, S. (2019): Association of body mass index with mortality in cardiovascular disease: new insights into the obesity paradox from multiple perspectives, Trends Cardiovasc. Med., 29: 220-225.

Young, D. (2001): Effect of disease on clinical lab Tests, 4th ed. AACC press.

Zhang, Y.; Proenca, R.;Maffe, i.; M., Barone, M.; Leopold, L. and Friedman, J.M. (1995): positional cloning of the mouse obese gene and its human homologue, [erratum 374:479]. Nature. 72:425-432.