Antigenic evaluation of extracted fimbrial protein obtained from pathogenic Escherichia coli isolated from diarrheic camel neonates

Amany N. Dapgh; Momtaz A. Shahein; Essam Kamel; Samah F. Ali; Aml M. Ragab; Ashraf S. Hakim; Eman A. Khairy; Hussien A Abouelhag; Ehab A. Fouad

doi:10.53730/ijhs.v6nS6.12565

Authors

Amany N. Dapgh Animal Health Research Institute, Agriculture Research Center, Dokki, Giza
Momtaz A. Shahein Animal Health Research Institute, Agriculture Research Center, Dokki, Giza
Essam Kamel Animal Health Research Institute, Agriculture Research Center, Dokki, Giza
Samah F. Ali Animal Health Research Institute, Agriculture Research Center, Dokki, Giza
Aml M. Ragab Bacteriology department, Animal Health Research Institute, Tanta Branch, Agriculture Research Center
Ashraf S. Hakim
migris410@yahoo.com
Department of Microbiology and Immunology, National Research Centre, 33 Bohouth St., Dokki, Cairo, Egypt
Eman A. Khairy Department of Microbiology and Immunology, National Research Centre, 33 Bohouth St., Dokki, Cairo, Egypt
Hussien A. Abouelhag Assistant Professor of Microbiology and Immunology, National Research Centre, Egypt
Ehab A. Fouad Department of Microbiology and Immunology, National Research Centre, 33 Bohouth St., Dokki, Cairo, Egypt

Keywords:

camel calves, entropathogenic Escherichia colienterotoxigenic, Escherichia coli, fimbrial protein, immunoblot, SDS PAGE-electrophoresis

Abstract

Enteropathogenic Escherichia coli (EPEC) were considered prime reason of diarrhea among neonatal livestock animals in developing countries and could be of public health importance via contaminated milk and meat. Continual attempts were conducted to combat this illness using various antigenic determinants. This study was performed on four E. coli serotypes O26, O45, O103 and O111, which were previously recovered from diarrheic camel calves in Giza, Egypt. Extraction of fimbrial proteins was carried out through dialysis then evaluation of their immunogenic activity was preceded. SDS-PAGE electrophoresis was performed on crude extracted fimbrial proteins and revealed single band for each isolate ranged from 22 to 33kDa. Immunobloting was implemented on the extracted crude fimbrial proteins against E.coli O26 antisera formerly prepared in rabbit.These findings suggested that the fimbrial proteins are of immunogenicity importance and can serve as a protective passive vaccine antigen in prevention of diarrhea caused by EPEC and ETEC infection in camel calves.

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References

AsadiKaram MR, Habibi M and Bouzari S. 2019. Urinary tract infection: Pathogenicity, antibiotic resistance and development of effective vaccines against UropathogenicEscherichia coli. MolImmunol,108:56-67. doi: 10.1016/j.molimm.2019.02.007.

Bai Y, Wang G, Qi H, Wang Y, Xu C, Yue L, Hou X and Yu L. 2020. Immunogenicity of 987P fimbriae of enterotoxigenicEscherichia coli surface-displayed on Lactobacillus casei.Res Vet Sci,128:308-314. doi: 10.1016/j.rvsc.2019.12.016.

Bin P, Tang Z, Liu S, Chen S, Xia Y, Liu J, Wu H and Zhu G. 2018. Intestinal microbiota mediates EnterotoxigenicEscherichia coli-induced diarrhea in piglets. BMC Vet Res,5; 14(1):385. doi: 10.1186/s12917-018-1704-9.

Blackburn SA, Shepherd M and Robinson GK. 2021. Reciprocal Packaging of the Main Structural Proteins of Type 1 Fimbriae and Flagella in the Outer Membrane Vesicles of "Wild type" Escherichia coli Strains. Front Microbiol,12:557455. doi:10.3389/fmicb.2021.557455

Bourgeois AL, Wierzba TF and Walker RI. 2016. Status of vaccine research and development for enterotoxigenicEscherichia coli. Vaccine, 3; 34(26):2880-2886. doi: 10.1016/j.vaccine.2016.02.076.

Brade L, Rietschel ET, Kusumoto S, Shiba T andBrade H. 1986. Immunogenicity and antigenicity of synthetic Escherichia coli lipid A.Infect Immun,51 (1):110-114.

Buckles EL, Bahrani-Mougeot FK, Molina A, Lockatell CV, Johnson DE, Drachenberg CB, Burland V, Blattner FR andDonnenberg MS. 2004. Identification and characterization of a novel uropathogenicEscherichia coli-associated fimbrial gene cluster. Infect Immun,72(7):3890-901. doi: 10.1128/IAI.72.7.3890-3901.2004.

Duguid JP, Smith IW, Dempster G and Edmunds PN. 1955. Non flagellar filamentous appendages “Fimbriae” and haemagglutinating activity in Bacterium coli. J PatholBacteriol, 90: 335-348.

Galal HM, Hakim AS andDorgham SM. 2013. Phenotypic and virulence genes screening of Escherichia coli strains isolated from different sources in delta Egypt. Life Sci J,10(2):352-361.

Hakim AS, Omara ST, Syame SM and Fouad EA. 2017. Serotyping, antibiotic susceptibility, and virulence genes screening of Escherichia coli isolates obtained from diarrheic buffalo calves in Egyptian farms, Vet World,10(7): 769-773.

Hussain, S. A., Badekila, H., Honnani, S. S., & Eram, A. (2022). A study of clinical utility of peri operative c- reactive protein testing in general surgery at tertiary care centre. International Journal of Health Sciences, 6(S7), 301–311. https://doi.org/10.53730/ijhs.v6n7.10952

Laemmli U. 1970.Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227: 680–685.

Luiz WB, Rodrigues JF, Crabb JH, Savarino SJ and Ferreira LCS. 2015. Maternal vaccination with a fimbrial tip adhesin and passive protection of neonatal mice against lethal human enterotoxigenicEscherichia coli challenge. Infect Immun,83:4555–4564. doi:10.1128/IAI.00858-15.

Lymberopoulos MH, Houle S, Daigle F, Léveillé S, Brée A, Moulin-Schouleur M, Johnson JR andDozois CM. 2006. Characterization of Stg fimbriae from an avian pathogenic Escherichia coli O78:K80 strain and assessment of their contribution to colonization of the chicken respiratory tract. J Bacteriol,188(18):6449-59. doi: 10.1128/JB.00453-06.

Mainil J. 2013. Escherichia coli virulence factors.Vet ImmunolImmunopathol, 15; 152(1-2):2-12. doi: 10.1016/j.vetimm.2012.09.032.

Mataram, I. K. A., Laraeni, Y., & Agustini, N. P. (2017). Formula kahiguru high protein for making of food supplement as elimination stunting. International Journal of Life Sciences, 1(3), 14–27. https://doi.org/10.21744/ijls.v1i3.58

Nataro JP.2005. EnteroaggregativeEscherichia coli pathogenesis.CurrOpinGastroenterol,21: 4-8.

Rasko DA, Rosovitz MJ, Myers GS, Mongodin EF, Fricke WF, Gajer P, Crabtree J, Sebaihia M, Thomson NR, Chaudhuri R, Henderson IR, Sperandio V and Ravel J. 2008. The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates. J Bacteriol,190: 6881-6893.

Rossi E, Cimdins A, Lüthje P, Brauner A, Sjöling Å, Landini P and Römling U. 2018. It's a gut feeling; Escherichia coli biofilm formation in the gastrointestinal tract environment. Crit Rev Microbiol,44(1):1-30. doi: 10.1080/1040841X.2017.1303660.

Sahl JW, Sistrunk JR, Fraser CM, Hine E, Baby N, Begum Y, Luo Q, Sheikh A, Qadri F, Fleckenstein JM andRasko DA. 2015. Examination of the EnterotoxigenicEscherichia coli Population Structure during Human Infection.mBio, 9; 6(3):e00501.

Sarkar S, Hutton ML andVagenas D. 2018.Intestinal Colonization Traits of Pandemic Multidrug-Resistant Escherichia coli ST131. J Infect Dis,218(6):979-990. doi:10.1093/infdis/jiy031.

Shahein MA, Dapgh AN, Kamel E, Ali SF, Khairy EA, Abuelhag HA and Hakim AS. 2021. Advanced molecular characterization of enteropathogenicEscherichia coli isolated from diarrheic camel neonates in Egypt.Vet World,14(1): 85-91.

Sheikh A, Rashu R, Begum YA, Kuhlman FM, Ciorba MA, Hultgren SJ, Qadri F and Fleckenstein JM. 2017. Highly conserved type 1 pili promote enterotoxigenicE. coli pathogen-host interactions. PLoSNegl Trop Dis, 22;11(5):e0005586. doi: 10.1371/journal.pntd.0005586.

Sincock SA, Hall ER, Woods CM, O'Dowd A, Poole ST, McVeigh AL, Nunez G, Espinoza N, Miller M and Savarino SJ. 2016. Immunogenicity of a prototype enterotoxigenicEscherichia coliadhesin vaccine in mice and nonhuman primates. Vaccine, 4;34(2):284-291. doi: 10.1016/j.vaccine.2015.11.017.

Song J, Zhao L and Song MA. 2020. Lactococcuslactis-vectored oral vaccine induces protective immunity of mice against enterotoxigenicEscherichia coli lethal challenge. Immunol Lett,225:57-63. doi: 10.1016/j.imlet.2020.06.007.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). The COVID-19 pandemic. International Journal of Health Sciences, 5(2), vi-ix. https://doi.org/10.53730/ijhs.v5n2.2937

Zhang H, Xu Y, Zhang Z, You J, Yang Y and Li X. 2018. Protective immunity of a Multivalent Vaccine Candidate against piglet diarrhea caused by enterotoxigenicEscherichia coli (ETEC) in a pig model. Vaccine, 29; 36(5):723-728. doi: 10.1016/j.vaccine.2017.12.026.