Effect of folic acid supplementation to Lohmann Brown layers diet on performance, egg quality and folic acid content of eggs and serum
Keywords:
folic acid, serum, egg, egg quality, Lohmann layerAbstract
Sometimes, customers added folic acid to layer diet to enhance brown egg shell color. This supplementation may lead to increase the content of folic acid in serum and eggs. The increment of folic acid in egg lead to produce folate egg that it will be beneficial to customers health especially pregnant women. This study was carried out to investigate the effect of supplementation of folic acid enriched diet on layer performance, egg quality, and folic acid content of egg and serum. Five thousand Lohmann classic brown laying hen’s type were use in this experiment. At the first period, hens were fed on basal diet according to Lohmann Classic Brown nutrition requirement guide. The Second period of the experiment, all hens were fed same basal diet enriched with folic acid with 1mg/kg of feed. Eggs were collected and blood samples were collected from the layers at the end of the first and second periods for measuring folic acid content of egg and serum by using HPLC & ELIZA respectively. Egg quality were measuring by calculating of egg weight, albumin height, albumin diameter, yolk height, yolk diameter, yolk color, shell thickness and Haugh unit.
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Abbasi, I. H. R., Abbasi, F., Wang, L., Abd El Hack, M. E., Swelum, A. A., Hao, R., ... & Cao, Y. (2018). Folate promotes S-adenosyl methionine reactions and the microbial methylation cycle and boosts ruminants production and reproduction. Amb Express, 8(1), 1-10.
Altic, L., McNulty, H., Hoey, L., McAnena, L., & Pentieva, K. (2016). Validation of folate-enriched eggs as a functional food for improving folate intake in consumers. nutrients, 8(12), 777.
Arzeni, C., Pérez, O. E., LeBlanc, J. G., & Pilosof, A. M. (2015). Egg albumin–folic acid nanocomplexes: Performance as a functional ingredient and biological activity. Journal of functional foods, 18, 379-386.
Bagheri, S., Janmohammadi, H., Maleki, R., Ostadrahimi, A., & Kianfar, R. (2019). Laying hen performance, egg quality improved and yolk 5-methyltetrahydrofolate content increased by dietary supplementation of folic acid. Animal nutrition, 5(2), 130-133.
Bailey, L. B., Stover, P. J., McNulty, H., Fenech, M. F., Gregory III, J. F., Mills, J. L., ... & Raiten, D. J. (2015). Biomarkers of nutrition for development—folate review. The Journal of nutrition, 145(7), 1636S-1680S.
Choi, J. H., Yates, Z., Veysey, M., Heo, Y. R., & Lucock, M. (2014). Contemporary issues surrounding folic acid fortification initiatives. Preventive nutrition and food science, 19(4), 247.
Cui, Z., Amevor, F. K., Feng, Q., Kang, X., Song, W., Zhu, Q., ... & Zhao, X. (2020). Sexual maturity promotes yolk precursor synthesis and follicle development in hens via liver-blood-ovary signal axis. Animals, 10(12), 2348.
Czarnowska-Kujawska, M., Draszanowska, A., Gujska, E., Klepacka, J., & Kasińska, M. (2021). Folate content and yolk color of hen eggs from different farming systems. Molecules, 26(4), 1034.
Dickson, T. M., Tactacan, G. B., Hebert, K., Guenter, W., & House, J. D. (2010). Optimization of folate deposition in eggs through dietary supplementation of folic acid over the entire production cycle of Hy-Line W36, Hy-Line W98, and CV20 laying hens. Journal of Applied Poultry Research, 19(1), 80-91.
DIRECTIVE, H. A. T. (2007). DIRECTIVE 2007/…/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL.
Grodnitskaya, E. E., & Kurtser, M. A. (2012). Homocysteine metabolism in polycystic ovary syndrome. Gynecological Endocrinology, 28(3), 186-189.
Gu, L., Yang, Y., McClements, D. J., Dong, S., Liu, Y., Li, J., ... & Su, Y. (2022). Preparation and characterization of folate-enriched eggs and egg yolk powders. Journal of Agriculture and Food Research, 8, 100309.
Hebert, K., House, J. D., & Guenter, W. (2005). Effect of dietary folic acid supplementation on egg folate content and the performance and folate status of two strains of laying hens. Poultry science, 84(10), 1533-1538.
Hoey, L., McNulty, H., McCann, E. M., McCracken, K. J., Scott, J. M., Marc, B. B., ... & Pentieva, K. (2008). Laying hens can convert high doses of folic acid added to the feed into natural folates in eggs providing a novel source of food folate. British journal of nutrition, 101(2), 206-212.
House, J. D., Braun, K., Ballance, D. M., O'connor, C. P., & Guenter, W. (2002). The enrichment of eggs with folic acid through supplementation of the laying hen diet. Poultry Science, 81(9), 1332-1337.
Islam I., Omara M., Ramy M., Romeilah M. 2009. Energy and methionine utilization in laying hen diets supplementation with folic acid. Res J Agric Biol Sci. ;5(4):428–444.
Munyaka, P. M., Tactacan, G., Jing, M., House, J. D., & Rodriguez-Lecompte, J. C. (2012). Immunomodulation in young laying hens by dietary folic acid and acute immune responses after challenge with Escherichia coli lipopolysaccharide. Poultry Science, 91(10), 2454-2463.
Nimalaratne, C., & Wu, J. (2015). Hen egg as an antioxidant food commodity: A review. Nutrients, 7(10), 8274-8293
North, M.O. and D.D. Bell, 1990. Commercial chicken production manual. 4th Edn., AVi, New York.
Saleh, M., Kokoszyński, D., Mousa, M. A. A., & Abuoghaba, A. A. K. (2021). Effect of Date Palm Pollen Supplementation on the Egg Production, Ovarian Follicles Development, Hematological Variables and Hormonal Profile of Laying Hens. Animals, 11(1), 69.
Tactacan, G. B., Jing, M., Thiessen, S., Rodriguez-Lecompte, J. C., o’Connor, D. L., Guenter, W., & House, J. D. (2010). Characterization of folate-dependent enzymes and indices of folate status in laying hens supplemented with folic acid or 5-methyltetrahydrofolate. Poultry science, 89(4), 688-696.
Wallock-Montelius, L. M., Villanueva, J. A., Chapin, R. E., Conley, A. J., Nguyen, H. P., Ames, B. N., & Halsted, C. H. (2007). Chronic ethanol perturbs testicular folate metabolism and dietary folate deficiency reduces sex hormone levels in the Yucatan micropig. Biology of reproduction, 76(3), 455-465.
Yajnik, C. S., & Deshmukh, U. S. (2008). Maternal nutrition, intrauterine programming and consequential risks in the offspring. Reviews in Endocrine and Metabolic Disorders, 9(3), 203-211.
Zaheer, K. (2017). Hen egg carotenoids (lutein and zeaxanthin) and nutritional impacts on human health: a review. CYTA-Journal of Food, 15(3), 474-487.
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