Effect of folic acid supplementation to Lohmann Brown layers diet on immune response and intestinal morphology

Abeer K. Al-Shimary; Yasser J. Jameel

doi:10.53730/ijhs.v6nS9.13577

Authors

Abeer K. Al-Shimary Department of Public Health/ College of Veterinary Medicine, University of Kerbala, Karbala, Iraq.
Yasser J. Jameel Department of Public Health/ College of Veterinary Medicine, University of Kerbala, Karbala, Iraq.

Keywords:

folic acid, immune response, villi height, crypt depth, Lohmann hens

Abstract

Sometimes, producer use the folic acid to enhance shell brown color of the brown hens` eggs. Therefore, this study was carried out to investigate the effect of supplementation of folic acid enriched diet on immune response and intestinal morphology of laying hens. Five thousand Lohmann Classic Brown laying hen type were use in this experiment. At the first period, hens were fed on basal diet according to Lohmann Classic Brown nutrition requirement guide. The Second period of the experiment, all hens were fed same basal diet enriched with folic acid with 1mg/kg of feed. Blood samples were collected from the hens at the end of the first and second periods for measuring the effect of folic acid on immune response which tested by using ELIZA technique. Duodenum, jejunum and ileum were taken from intestine for measuring villi height and crypt depth. Immune tolerance of ND showed a significant increase after administrated folic acid as compare with before administration folic acid. Villi height and crept depth showed a significant difference in second periods after supplementation of folic acid compare with first period before folic acid supplementation.

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