Multiplex PCR for detection of intestinal parasites in patients at Wasit province

Magda A. Ali; Ghaidaa M. Naji

doi:10.53730/ijhs.v6nS8.13677

Authors

Magda A. Ali Department of Biology, College of Science, Wasit University
Ghaidaa M. Naji Department of Biology, College of Science, Wasit University

Keywords:

intestinal parasite, human, multiplex PCR, feces

Abstract

Intestinal parasitic infestation still represents an economic and public health problem in the world particularly in the developing countries including the Middle East .To estimate the current prevalence of intestinal parasitic infection among people living in Wasit province/ middle of Iraq .100 fecal samples were collected from males and females of different ages attending AL-Karama Teaching Hospital suffering of gastroenteritis symptoms during the period from August to November 202, only 45 stool sample were positive for intestinal parasite. The total infectivity rate by intestinal parasites was (45%).This study indicated high occurrence of gastro-intestinal parasites in Wasit province, Iraq. The overall infection rate by intestinal protozoa was significantly higher than intestinal helminth infection. In the present study prevalence of intestinal parasitic infection was Entamoeba histolytica 15(33.3%). the percent of the infection among males were 9(20%) while the females were 6(13.3%). According the residence in urban areas were reported 5( 11.1%) and rural areas were 10(22.2%). The Co-infection appears in 19 samples with percent of infection (42.2%) in males infection were predominance 11(24.4%) than the females 8(17.7%). According to the residence urban areas were reported 7 (15.5%) and rural areas were 12(26.6%).

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