Nested-PCR for diagnosis of Hymenolepis nana in the patients of Wasit Province

https://doi.org/10.53730/ijhs.v6nS2.8700

Authors

  • Magda A. Ali Wasit University / College of Science / Iraq
  • Sarah S. Zghair Wasit University / College of Science / Iraq

Keywords:

H.nana, feces, human, nested-PCR

Abstract

The current study was carried out at Wasit Province / Iraq in cooperation with AL-Karama Teaching Hospital. Samples were collected from many places at Wasit Province. It included 100 stool samples from Iraqi patients conducted during  the period from October to December, 2021 to investigate the prevalence of  Hymenolepis nana. Only 23 stool sample were positive for H.nana. These samples were collected from the villages and rural areas of Wasit Province, the villages of Sayed-Shati and Al-Battar, the area of al-Anwar, the peasants, the expatriates, and the Dujaili area. The results showed that the percentage of H.nana infection among patients were 23 (23%).  With regard to parasitic infection, the highest infection rate was recorded for the group (4-15) years old, as for sex, the percentage of males reached about 78.26% and females 21.74% in this study with no significant differences.  According to residence, The highest infection was appeared in the rural areas 75% and the lowest was in the urban areas 25% with a significant differences.

Downloads

Download data is not yet available.

References

Cheng T, Liu GH, Song HQ, Lin RQ, Zhu XQ. The complete mitochondrial genome of the dwarf tapeworm Hymenolepis nana—a neglected zoonotic helminth. Parasitology Research. 2016; 115(3): 1253-62.

Sharma S, Lyngdoh D, Roy B, Tandon V. Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker. Parasitology research. 2016; 115(11): 4293-8.

Goodwin, B.S.; Yarbrough, L.W. AND Head, K.L. (2004). Rats and mice: Parasitic diseases. Laboratory animal medicine and science series II, V-9045. Health sciences center for educational resources, University of Washington.

Ortega, Y.R. (2006). Foodborn parasites. Springer Science + Business Media LLC. USA.

CDC (2010). DPDx: Laboratory Identification of Parasites of Public Health Concern. Atlanta: Center for Disease Control & Prevention, USA.

Pinto, R.M.; Vicente, J.J.; Noronha, D.; Goncalves, L. and Gomes, D.C. (1994). Helminth parasites of conventionally Maintained Laboratory Mice. Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol.89, No.1, pp.33-40.

Parija SC. Textbook of Medical Parasitology, Protozoology and Helminthology. Pashupati Printers, New Delhi, second edition; 2004.

Becker SL, Lohourignon LK, Speich B, Rinaldi L, Knopp S, N’Goran EK et al. Comparison the flotac-400 dual technique and formalin-ether concentration technique for diagnosis of Human intestinal protozoan infection. J. clin. Microbiol 2011; 49(6): 2183-2190.

Kremer M and Molet B. Interêt de la technique de Kato en coprologie parasitaire. Anna. Soc. Belge Med. Trop 1975; 55(5): 427-430.

Beaver PR and Jung EC. Clinical Parasitology, Ninth Edition. Philadelphia: Lea and Febiger. 1984.

Tresnani, G.; Suana, I. W. and Hadi, I. (2016). The Detection of Hymenolepis sp. from House Rats (Rattus rattus diardii Jentink, 1880) in Mataram through ITS-1 Gene PCR Analysis. American Institute of Physics. 978-0-7354-1401-3.

Nkouawa A, Haukisalmi V, Li T, Nakao M, Lavikainen A, Chen X, Henttonen H, Ito A. Cryptic diversity in hymenolepidid tapeworms infecting humans. Parasitology International. 2016; 65(2): 83-6.

Yamasaki H, Nakaya K, Nakao M, Sako Y, Ito A. Significance of molecular diagnosis using histopathological specimens in cestode zoonoses. Tropical Medicine and Health. 2007; 35(4): 307-21.

Kheirandish F, Tarahi MJ, Haghighi A, Nazemalhosseini-Mojarad E, Kheirandish M.Prevalence of intestinal parasites in bakery workers in Khorramabad, Lorestan Iran. Iranian Journal of Parasitology. 2011; 6(4): 76.

Kheirandish F, Tarahi MJ, Ezatpour B. Prevalence of intestinal parasites among food handlers in Western Iran. Revista do Instituto de Medicina Tropical de São Paulo. 2014; 56(2): 111-4.

Badparva E, Kheirandish F, Ebrahimzade F. Prevalence of intestinal parasites in Lorestan Province, West of Iran. Asian Pacific Journal of Tropical Disease. 2014; 4: 728-32.

Rostami M, Tohidi F, Sharbatkhori M, Taherkhani H. Intestinal parasitic infection among school children in Golestan province. Iran. Pak J Biol Sci. 2012; 15(23): 119- 1125.

Tappe KH, Mohammadzadeh H, Khashaveh S, Rezapour B, Barazesh A. Prevalence of intestinal parasitic infections among primary school attending students in Barandooz-Chay rural region of Urmia, West Azerbaijan province, Iran in 2008. African Journal of Microbiology Research. 2011; 5(7): 788-91.

Sirivichayakul C, Radomyos PR, Praevanit R, Jojjaroen-Anant C, Wisetsing P. Hymenolepis nana infection in Thai children. Journal Medical Association of Thailand. 2000; 83(9): 1035-8.

Okamoto M, Agatsuma T, Kurosawa T, Ito A. Phylogenetic relationships of three hymenolepidid species inferred from nuclear ribosomal and mitochondrial DNA sequences. Parasitology. 1997; 115(6): 661-6.

Macnish MG, Morgan-Ryan UM, Monis PT, Behnke JM, Thompson RC. A molecular phylogeny of nuclear and mitochondrial sequences in Hymenolepis nana (Cestoda) supports the existence of a cryptic species. Parasitology. 2002; 125(6): 567-75.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). The COVID-19 pandemic. International Journal of Health Sciences, 5(2), vi-ix. https://doi.org/10.53730/ijhs.v5n2.2937

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Published

09-06-2022

How to Cite

Ali, M. A., & Zghair, S. S. (2022). Nested-PCR for diagnosis of Hymenolepis nana in the patients of Wasit Province. International Journal of Health Sciences, 6(S2), 14207–14213. https://doi.org/10.53730/ijhs.v6nS2.8700

Issue

Section

Peer Review Articles