Calibrated behaviors assessment in spinal cord compression injury model in rats

Farooq Khan; Najma Baseer; Hamid Habib; Noman Ullah; Salma Khalid; Saima Mumtaz

doi:10.53730/ijhs.v6nS9.14079

Authors

Farooq Khan Department of Anatomy, Mohammad College of Medicine, Pakistan
Najma Baseer
Drnajma.ibms@kmu.edu.pk
Department of Anatomy, Khyber Medical University, Peshawar, Khyber Pakhtunkhwa, Pakistan
Hamid Habib Department of Anatomy, Khyber Medical University, Peshawar, Khyber Pakhtunkhwa, Pakistan
Noman Ullah Department of Anatomy, Mohammad College of Medicine, Pakistan
Salma Khalid Prime Institute of Public Health, Prime Foundation, Riphah International University, Islamabad, Pakistan
Saima Mumtaz Federal Medical College, Islamabad, Pakistan

Keywords:

Thoracic level compression, spinal cord injury, compression of aneurysm clip

Abstract

Spinal cord injuries (SCI) are often associated with dire consequences resulting in the serious pathological conditions in SCI patients. These consequences have resulted in an increased interest of basic science researchers and healthcare professionals to investigate effective treatment of SCI. Among these initiatives, animal experimentation models emerged as a successful tool to study the SCI in detail. We created a standardized spinal cord compression injury model for this research and tested its effectiveness in examining the behaviors of rodents with SCI. We used 24 mature Sprague Dawley rats, weighing between 250 and 320g. Two sets, A and B, of rats were created. Under general anesthesia, Rat from group A underwent laminectomy and only had the T6–T8 vertebrae of their spinal arch removed. in contrast to the rats in group B underwent laminectomy as well as compression injuries using an aneurysm clip of 70 gm for 60 seconds. All the rats were observed at day 7, 14 and 28 following the experimentation. Using standardised techniques, the sensory-motor abilities were evaluated.

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