Antibacterial activity of Rhizopus specie isolated from rhizosphere of Mentha Piperita
Keywords:
Mentha piperita, antibacterial, antibiotics, bioactiveAbstract
Soil is loaded by microbes which are capable of producing antibiotics. These compounds were used therapeutically and sometimes prophylactically in the control of infectious disease. Soil microbes can be grouped into Bacteria, Actinomycetes, Fungi, Algae, Protozoa and Nematodes. Fungi are unique eukaryotes which ingest food from outside and take nutrients through its cell walls and consists of tiny tubular mycelia known as hyphae. The fungi are well known in medical field due to the production of a wide variety of secondary metabolites. The current research study was focused on isolation, identification and antibacterial activity of fungi isolated from rhizosphere of Mentha Piperita (Mint), Abasyn University, Peshawar. The research study was conducted in the Microbiology Research Laboratory (MRL) of Abasyn University Peshawar. The soil sample was collected from rhizosphere by using sterile techniques. Serial dilution method was used to dilute the concentration of microbes present in soil sample. The Potato Dextrose Agar media was prepared and by pour plate method the sample was streaked on the plate. The plates were incubated at 280C for 72hours. The fungus was isolated by sub culturing technique and then identified by morphological and microscopic examination using Lactophenol cotton blue stain (LPCB).
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