Evaluation of Penicillins minimum inhibitory concentration against clinically isolated Streptococcus pyogenes

Sidra Farooq; Mahar Un Nissa; Noor Us Saba; Madiha Iqbal; Iqbal Nisa; Mehboob Ullah; Muhsin Jamal; Nabila Qayum; Didar Ali Shah; Maria Gul Siddiqui; Amjad Khan; Farhad Khan

doi:10.53730/ijhs.v7nS1.14564

Authors

Sidra Farooq
sidra.farooq@abasyn.edu.pk
Department of Health and Biological Sciences, Abasyn University Peshawar
Mahar Un Nissa Department of Microbiology, University of Karachi, Pakistan
Noor Us Saba Department of Microbiology, University of Karachi, Pakistan
Madiha Iqbal Department of Health and Biological Sciences, Abasyn University Peshawar
Iqbal Nisa Department of Microbiology, Women university Swabi, Pakistan
Mehboob Ullah Department of Microbiology, University of Haripur, Pakistan
Muhsin Jamal Department of Microbiology, Abdul Wali Khan University, Garden Campus, Mardan Pakistan
Nabila Qayum Centre for Biotechnology and Microbiology, University of Swat, Pakistan
Didar Ali Shah Department of Microbiology, Hazara University, Manshera Pakistan
Maria Gul Siddiqui Department of Health and Biological Sciences, Abasyn University Peshawar
Amjad Khan Department of Health and Biological Sciences, Abasyn University Peshawar
Farhad Khan Centre for Biotechnology and Microbiology, University of Swat, Pakistan

Keywords:

Streptococcus pyogenes, pharyngitis, hemolysis, resistant, minimum inhibitory concentration

Abstract

Streptococcus specie is the crucial cause of pharyngitis and tonsillitis in human particularly in youngsters and is the main organisms transmitted from the milk to human. Streptococcus pyogenes cause diseases worldwide, thus increases huge burden to national health care systems. The current study was take on to determine antibiotic resistance pattern of S. pyogenes against penicillin group of antibiotics by disc diffusion method. Furthermore, minimum inhibitory concentration of penicillin group of antibiotics were assisted against S. pyogenes isolates. Three hundred samples including sore throat, skin lesion and surgical wounds were collected from different patients. The clinical specimens were cultured on blood agar media to observe hemolysis and were identified with Bacitracin sensitivity test. To evaluate the resistance pattern of Penicillin’s antibiotic sensitivity test was done. Different antibiotics used were Penicillin G (10 U), Ampicillin (10 mcg), Amoxicillin (25 mcg), and Piperacillin (100 mcg) respectively. Minimum inhibitory concentration was checked against S. pyogenes by using four antibiotics i.e. Penicillin G, Piperacillin, Ampicillin and Amoxicillin. A total of 17 isolates showed positive growth for S. pyogenes i.e. from throat specimens the number of positive isolates were 10, surgical wounds showed 05 and skin pus were positive for 02 isolates.

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