Storage conditions and collection containers for various specimen types to preserve analyte stability
Review
Keywords:
Biological fluids, Formaldehyde, Genetic material, Pre-analytical stageAbstract
Background: Molecular tests for detecting nucleic acids in biological materials are crucial in clinical diagnosis and therapy decision-making. However, errors in molecular labs often stem from pre-analytical mistakes during specimen handling. The integrity of nucleic acids can be compromised by inappropriate collection, transit, and storage procedures. Despite the importance of pre-analytic factors, there is a lack of standardized recommendations for molecular assays. Aim of Work: This article aims to provide a comprehensive overview of key considerations in sample handling before molecular testing, covering various types of biological samples. Methods: We conducted a review of the literature to identify key pre-analytical factors that can impact molecular testing. We focused on specimen collection, transportation, and storage protocols for solid tissues, whole blood, serum, plasma, and other bodily fluids. We also discussed pre-analytical factors relevant to prenatal diagnostic samples. Additionally, we highlighted the importance of adhering to established procedures to minimize errors in molecular testing. Results: Our review identified several critical pre-analytical factors that can affect the quality of nucleic acids in biological samples. Factors such as inadequate sample collection, improper transportation conditions, and suboptimal storage can lead to degraded nucleic acids, affecting the accuracy of molecular tests.
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