Impacts of Dexamethazone on Progesterone, Calcitonin, and Progesterone Receptors in Domestic Pregnant Rabbit (Oryctolagus cuniculus)

Amal Faisal Alabedi; Akram Yousuf Yasser; Alaa Jawad Hassan

doi:10.53730/ijhs.v6nS1.5839

Authors

Amal Faisal Alabedi
jakhahnoaman@agre.uoqasim.edu.iq
Department of Animal Production, College of Agriculture, Al-Qasim Green University
Akram Yousuf Yasser Department of Dentistry, Hilla University College
Alaa Jawad Hassan Department of Biology, College of Science, University of Babylon

Keywords:

Dexamethasone, Progesterone, Calcitonin and Progesterone Receptors, Domestic Pregnant Rabbits (Oryctolagus cuniculus)

Abstract

Dexamethasone (DEX) is used to treat several diseases and medical conditions in both animals and humans. But the administration of synthetic glucocorticoids, including dexamethasone, as a treatment, brings about endocrine misbalance. Such a disequilibrium underlies pregnancy complications. The current study investigates effects of High Dose of DEX (HD) (1.125 mg) and Low Dose of DEX (LD) (0.562 mg) on Progesterone (P), Progesterone Receptors (PR) and Calcitonin (C) in domestic pregnant does (Oryctolagus cuniculus). Sixty pregnant does were caged in the animal house and divided into six groups; A, B, C, D, E and F (n=10). These groups are treated as follows: Groups A and B (as an experimental groups) were daily injected by (1ml) from day 5 to 9 of gestation (dG) with HD and LD, respectively; whereas group C was injected by (1 ml) of sodium chloride (0.9%) (as a control group). Then, all the groups (A, B and C) were dissected at 10 and 28 dG.

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References

Whirledge SD, Cidlowski JA. Glucocorticoids and reproduction: trafﬁc control on the road to reproduction. Trends Endocrinol Metab. 2017 Jun;28(6):399-415. doi: 10.1016/j.tem.2017.02.005.

Erichsen MM, Husebye ES, Michelsen TM, Dahl AA, Løvås K. Sexuality and fertility in women with addison’s disease. J Clin Endo Met. 2010 Sep 1;95(9): 4354–4360. https://doi.org/10.1210/jc.2010-0445

Boomsma CM, Keay SD, Macklon NS. Peri-implantation glucocorticoid administration for assisted reproductive technology cycles. Cochrane Database Syst Rev. 2007 Jan 24;(1):CD005996. doi: 10.1002/14651858.CD005996.pub2.

Wang W, Guo C, Zhu P, Lu J, Li W, Liu C, Xie H, Myatt L, Chen Z, Sun K. Phosphorylation of STAT3 mediates the induction of cyclooxygenase-2 by cortisol in the human amnion at parturition. Sci Signal. 2015 Oct 27;8(400):ra106. doi: 10.1126/scisignal.aac6151.

Wang W, Liu C, Sun K. Induction of amnion epithelial apoptosis by cortisol via tpa/plasmin system. Endo. 2016 Nov;157(11):4487-4498. doi: 10.1210/en.2016-1464.

Geraghty AC, Kaufer D. Glucocorticoid regulation of reproduction. Adv Exp Med Biol. 2015; 872:253-78. doi: 10.1007/978-1-4939-2895-8_11.

Cable JK, Grider MH. Physiology, Progesterone. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan. PMID: 32644386. Bookshelf ID: NBK558960.

Yahi D, Ojo NA, Mshelia GD. Influence of dexamethasone on some reproductive hormones and uterine progesterone receptor localization in pregnant yankasa sheep in semiarid zones of nigeria. J Veterin Med. 2017 Article ID 9514861. https://doi.org/10.1155/2017/9514861

Shah NM, Lai PF, Imami N, Johnson MR. Progesterone-related immune modulation of pregnancy and labor. Front Endocrinol (Lausanne). 2019 Mar 29;10:198. doi: 10.3389/fendo.2019.00198.

Griesinger G, Tournaye H, Macklon N, Petraglia F, Arck P, Blockeel C. Dydrogesterone: pharmacological profile and mechanism of action as luteal phase support in assisted reproduction. Reprod Biomed Online. 2019 Feb;38(2):249-259. doi: 10.1016/j.rbmo.2018.11.017.

Yahi1 D, Ojo NA, Mshelia GD. Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in sahel goat in semi-arid region. J Anim Sci Technol. 2017 May 26;59:12. doi: 10.1186/s40781-017-0137-5.

Khan YS, Farhana A. Histology, Thyroid Gland. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan. PMID: 31869123. Bookshelf ID: NBK551659

Zhu LJ, Cullinan-Bove K, Polihronis M, Bagchi MK, Bagchi IC. Calcitonin is a progesterone-regulated marker that forecasts the receptive state of endometrium during implantation. Endo. 1998 Sep 1; 139(9):3923–3934. https://doi.org/10.1210/endo.139.9.6178

Kodaman PH, Taylor HS. Hormonal regulation of implantation. Obstet Gynecol Clin North Am. 2004 Dec;31(4):745-66, ix. doi: 10.1016/j.ogc.2004.08.008.

Bagchi IC, Li Q, Cheon YP. Role of steroid hormone-regulated genes in implantation. Ann N Y Acad Sci. 2001 Sep; 943:68-76. doi: 10.1111/j.1749-6632. 2001.tb03792. x.

Hoffman LH, Davenport GR, Brash AR. Endometrial prostaglandins and phospholipase activity related to implantation in rabbits: Effects of dexamethasone. Biol Reprod. 1984 Apr 1;30(3):544–555. https://doi.org/10.1095/biolreprod30.3.544

Ahmadabad HN, Jafari SK, Firizi MN, Abbaspour AR, Gharib FG, Ghobadi Y, Gholizadeh S. Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy. Clin Exp Reprod Med. 2016 Mar;43(1):15-25. doi: 10.5653/cerm.2016.43.1.15.

Luo E, Shannon BZS, Sharon C, Nagambika M, Alexander SK, Kellie MB. Corticosterone Blocks ovarian cyclicity and the LH surge via decreased kisspeptin neuron activation in female mice. Endo. 2016 Mar 1;157(3):1187–1199. https://doi.org/10.1210/en.2015-1711.

McDonald TJ, Franko KL, Brown JM, Jenkins SL, Nathanielsz PW, Nijland MJ. Betamethasone in the last week of pregnancy causes fetal growth retardation but not adult hypertension in rats. J Soc Gynecol Investig. 2003 Dec;10(8):469-73. doi: 10.1016/s1071-5576(03)00151-5.

Attardi BJ, Zeleznik A, Simhan H, Chiao JP, Mattison DR, Caritis SN. Comparison of progesterone and glucocorticoid receptor binding and stimulation of gene expression by progesterone, 17-alpha hydroxyprogesterone caproate, and related progestins. Am J Obstet Gynecol. 2007 Dec;197(6): 599.e1-7. doi: 10.1016/j.ajog.2007.05.024.

Solano ME, Arck PC. Steroids, pregnancy and fetal development. Front Immunol. 2020 Jan 22;10:3017. doi: 10.3389/fimmu.2019.03017.

Issar M, Sahasranaman S, Buchwald P, Hochhaus G. Differences in the glucocorticoid to progesterone receptor selectivity of inhaled glucocorticoids. Eur Respir J. 2006 Mar;27(3):511-6. doi: 10.1183/09031936.06.00060005.

Whirledge SD, Oakley RH, Myers PH, Lydon JP, DeMayo F, Cidlowski JA. Uterine glucocorticoid receptors are critical for fertility in mice through control of embryo implantation and decidualization. Proc Natl Acad Sci U S A. 2015 Dec 8;112(49):15166-71. doi: 10.1073/pnas.1508056112.

Lissauer D, Eldershaw SA, Inman CF, Coomarasamy A, Moss PA, Kilby MD. Progesterone promotes maternal-fetal tolerance by reducing human maternal t-cell polyfunctionality and inducing a specific cytokine profile. Eur J Immunol. 2015 Oct;45(10):2858-72. doi: 10.1002/eji.201445404.

Engler JB, Kursawe N, Solano ME, Patas K, Wehrmann S, Heckmann N. Glucocorticoid receptor in t cells mediates protection from autoimmunity in pregnancy. Proc Natl Acad Sci U S A. 2017 Jan 10;114(2): E181-E190. doi: 10.1073/pnas.1617115114.

Arenas-Hernandez M, Romero R, Xu Y, Panaitescu B, Garcia-Flores V, Miller D. Effector and activated t cells induce preterm labor and birth that is prevented by treatment with progesterone. J Immunol. 2019 May 1;202(9):2585-2608. doi: 10.4049/jimmunol.1801350.

Cote GJ, Gage RF. Dexamethasone differentially affects the levels of calcitonin and calcitonin gene-related peptide mrnas expressed in a human medullary thyroid carcinoma cell line. J Biol Chem. 1986 Nov 25;261(33):15524-8. PMID: 3491070.

Silva OL, Titus-Dillon P, Becker KL, Snider RH, Moore CF. Increased serum calcitonin in pregnancy. J Natl Med Assoc. 1981;73(7):649-52. PMID: 7265273

Muszynski M, Birnbaum R, Roos B. Glucocorticoids stimulate the production of preprocalcitonin-derived secretory peptides by a rat medullary thyroid carcinoma cell line. J Biol Chem. 1983 Oct 10;258(19):11678-83.