Quantification of antibiotic residues in raw and layers hen eggs by rp-hplc
Keywords:
Antibiotic, oxytetracycline, enrofloxacin, Maximum residue limit, HPLCAbstract
Antibiotics (Oxytetracycline & Enrofloxacin) residual levels in raw and layered samples are to be quantified using RP-HPLC. The antibiotic (oxytetracycline and enrofloxacin) residual levels in raw and layers hen egg samples were evaluated qualitatively by high performance liquid chromatography in this investigation, which used one hundred randomly selected samples (HPLC). C18 (Hypersil ODS-BPS, 250 4.6mm; 5) was used for the separation at a flow rate of 1 ml/min in a mobile phase of 0.1 percent formic acid: acetonitrile (50:50, v/v). At a detection wavelength of 350 nm, the residues were measured. Found oxytetracycline residues, which indicate widespread usage of antibiotics on farms and a lack of application of required withdrawal durations, were detected in samples. Because of this, the antibiotic residues found in broiler hen eggs have been reduced. Restrictive methods and stricter restrictions should be implemented to prevent the presence of abuse residues prior to marketing, according to these findings.
Downloads
References
Abd El. Monem, K. M.; Soliman, M. R. and Saad, S.M. (2002): Oxytetracycline residues in Broiler carcasses produced by closed and open system. Journal of Egyptian Veterinary Medical Association, 62 (6a): 119-124.
Anderson, A. M. and Skakkebaek, N. E. (1999):Exposure to exogenous estrogens in food: possibment and health. Eur. J. Endocrinol. 140(6): 477-485. DOI: https://doi.org/10.1530/eje.0.1400477
Caruso-Nicoletti, M.; Cassorla, F.; Skerda, M.; Ross, J. L.; Loriaux, D. L. and Cutler, G.B. (1985): Short term, low dose estradiol accelerates granular growth in boys. J.Clin.Endocrinol Metab.61 (5):896-8. DOI: https://doi.org/10.1210/jcem-61-5-896
Di Corcia, A. and Nazzari, M. (2002): Liquid chromatography–mass spectrometric methods for analyzing antibiotic and antibacterial agents in animal food products. Journal of Chromatography A, 974, 53–89. DOI: https://doi.org/10.1016/S0021-9673(02)00905-6
Donoghue, D. J. and Hairston, H. (2000): Food safety implication: certain antibiotics may rapidly contaminate egg albumen during the process of its formation. British Poultry Science, 41, 174–177. DOI: https://doi.org/10.1080/713654912
EC, European community (1996): Council directive 96/22/EC of 29 April 1996 concerning the prohibition on the use in stock farming of certain substances having a hormonal or thyrostatic action and of beta-agonists, and repealing directives 81/602/EEC, 88/146/EEC and 88/299/EEC. Official Journal of the European Communities, L125, 3–9.
EC, (2010): Commission regulation NO 37/2010 of 22, December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin, off. J. Eur. Communities. L 15, pp.1-7.
Fang, X.; Chen, J. and Guo, D. (2002): Detection and Identification of Zeranol in Chicken or Rabbit Liver by Liquid Chromatography Electrospray Tandem Mass Spectrometry. Journal of AOAC International. 85, NO. 4, 841847. DOI: https://doi.org/10.1093/jaoac/85.4.841
Gigososa, P. G.; Revesadoa, P. R.; Cadah´iaa, O.; Fenteb, C. A.; Vazquezb, B. I.; Francob, C.M. and Cepeda, A. (2000): Determination of quinolones in animal tissues and eggs by high performance liquid chromatography with photodiode-array detection. Journal of Chromatography A, 871 31–36. DOI: https://doi.org/10.1016/S0021-9673(99)01048-1
Javadi, A.; Mirzaei, H. and Khatibi, S. A. (2011): Effect of roasting, boiling and microwaving cooking methods on Enrofloxacin residues inedible tissues of brournal of Pharmacy and Pharmacology, 5(2): 214-218.
Kan, C. A. and Petz, M. (2000): Residues of veterinary drugs in eggs and their distribution between yolk and white. Journal of Agricultural and Food Chemistry, 48: 6397–6403. DOI: https://doi.org/10.1021/jf000145p
Yorke, J. C. and Froc, P. (2000): Quantitation of nine quinolones in chicken tissues by High performance liquid chromatography with fluorescence detection. J. Chromatography A, 882: 63-77. DOI: https://doi.org/10.1016/S0021-9673(00)00165-5
Lone, K. P. (1997): Natural sex steroids and their xenobiotic analogs in animal production: Growth, carcass quality, pharmacokinetics, metabolism, mode of action, residues, methods, and epidemiology. Critical Reviews in Food Science and Nutrition, 37(2): 93–209. DOI: https://doi.org/10.1080/10408399709527771
Schenck, F. J. and Callery, P. S. (1998): Chromatographic methods of analysis of antibiotics in milk. Journal of Chromatography A, 812, 99–109. DOI: https://doi.org/10.1016/S0021-9673(97)01291-0
Okerman, L.; Hende, J. V. and De Zutter, L. (2007): Stability of frozen stock solutions of betalactam antibiotics, cephalosporins, tetracycline’s and quinolones used in antibiotic residue screening and antibiotic susceptibility testing. Analytica Chimica Acta, 586: 284–288. DOI: https://doi.org/10.1016/j.aca.2006.10.034
Rose, M. D.; e, J.; Farrington, W. H. and Shearer, G. (1996): The effect of cooking on veterinary drug residues in food: 4.Oxytetracycline. Food Additives and Contaminants, 13 (3): 275– 286. DOI: https://doi.org/10.1080/02652039609374409
Young, L. L.; Northcutt J. K.; Buhr, R. J.; Lyon, C.E. and. Ware, G. O. (2001): Effects of Age, Sex and Duration of Postmortem Aging on Percentage Yield of Parts from Broiler Chicken Carcasses. Poultry Science 80:376–379. DOI: https://doi.org/10.1093/ps/80.3.376
Lolo, M.; Pedreira, S.; Vázquez Belda, B.; Miranda, J.; Franco, C.; Cepeda, A. and Fente, C. (2006): The effect of cooking on enrofiaxacin residues in chicken muscle. J. Food Additives and Contaminants, 23, 10: 988-993. DOI: https://doi.org/10.1080/02652030600904894
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.