Isolation and identification of Aspergillus fumigatus from Immunocompromised patients in AL- Najaf Province

https://doi.org/10.53730/ijhs.v6nS1.6240

Authors

  • Ehab Y. Jabber Dept. Biology, Faculty of science, Kufa University, Najaf, Iraq
  • Oday H. Kadhim Al janabi Dept. Microbiology, Faculty of Medicine, Babylon University, Babylon, Iraq
  • Zaytoon A. Al-khafaji Dept. Microbiology, Faculty of Medicine, Babylon University, Babylon, Iraq

Keywords:

Immunocompromised patients, Aspergillus fumigatus, Aspergillosis, saprophytic fungus, Otomycosis

Abstract

The present study was conducted to isolation and identification of Aspergillus fumigatus. Isolated from immunocompromised patients with (Diabetes, Cancer) by different identification methods including direct examination, laboratory culture and electron microscopy. During the period from (July to September 2016), a total of 50 swab were collected from immunocompromised patients with attending to the in AL- Sadder Medical City (the Center for Diabetes and Endocrinology) and (Euphrates middle Center for cancer diseases). In AL-Najaf Governorate, the samples were collected as following: 30 swab from the eyes of cancer patients who were suffering from prostate, leukemia, stomach and intestines cancer, also 20 swabs from the ears of diabetic patients. The percentage of female to male was as following, 29 females (58%) males and 21 (42%). In this study, the results show that the incidence of aspergillosis in women was higher than the male. The results of the present study reveal that the prevalence of aspergillosis from patient with cancer was more than diabetes patients which was 18 (66.66%) and 9 (33.33%), respectively.

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References

Pitt, J.I., Samson, R.A., Frisvad, J.C. (2000). In: Integration of Modern Taxonomic Methods for Penicillium and Aspergillus Classification (Eds. RA Samson and JI Pitt), Hardwood Academic Publishers, Reading, UK, 9- 50

Haines, J. (1995). Aspergillus in compost: straw man or fatal flaw. Biocycle 6:32–35.

Pitt, J. I. (1994). The current role of Aspergillus and Penicillium in human and animal health. J. Med. Vet. Mycol. S1:17–32. DOI: https://doi.org/10.1080/02681219480000701

Vanden Bossche, H.;Mackenzie, D. W. R. and Cauwenbergh ,G. (1988). Aspergillus and aspergillosis. Plenum Press, New York, N.Y DOI: https://doi.org/10.1007/978-1-4899-3505-2

Mullins, J.; Harvey,R. and Seaton,A.( 1976). Sources and incidence of airborne Aspergillus fumigatus (Fres). Clin. Allergy 6:209–217. DOI: https://doi.org/10.1111/j.1365-2222.1976.tb01899.x

Mullins, J.; Hutcheson, P. and Slavin,R. G.( 1984). Aspergillus fumigatus spore concentrations in outside air: Cardiff and St. Louis compared. Clin. Allergy 14:251–254. DOI: https://doi.org/10.1111/j.1365-2222.1984.tb02215.x

Nolard, N. (1994). Les liens entre les risques d’aspergillose et la contamination de l’environnement. Revue de la litte´rature. Pathol. Biol. 43:706–710.

Raper, K. B., and Fennell,D.(1965). Aspergillus fumigatus group, p. 238– 268. In K. B. Raper and D. I. Fennell (ed.), The genus Aspergillus. The William & Wilkins Co., Baltimore, Md.

Samson, R. A., and Van Reenen-Hoekstra,E. S.( 1988). Introduction to food-borne fungi. Centraalbureau voor Schimmelcultures, Baarn, Delft, The Netherlands.

Chazalet, V.; Debeaupuis, J. P. J.; Sarfati, J.; Lortholary, P.; Ribaud, P.; Shah, M.; Cornet, H. Vu.; Thien, E.; Gluckman, G.; Bru¨cker. and Latge J. P. (1998). Molecular typing of environmental and patient isolates of Aspergillus fumigatus fro various hospital settings. J. Clin. Microbiol. 36:1494–1500. DOI: https://doi.org/10.1128/JCM.36.6.1494-1500.1998

Goodley, J. M.; Clayton, Y. M. and Hay,R. J.( 1994). Environmental sampling for aspergilli during building construction on a hospital site. J. Hosp. Infect. 26:27–35. DOI: https://doi.org/10.1016/0195-6701(94)90076-0

Hospenthal, D. R.; Kwon-Chung, K. J. and Bennett,J. E.(1998). Concentrations of airborne Aspergillus compared to the incidence of invasive aspergillosis: lack of correlation. Med. Mycol. 36:165–168. DOI: https://doi.org/10.1080/02681219880000241

Sarfati, J.; Diaquin, M.; J. P.; Paris, S. and Latge, J. P. ´. Unpublished data.

Chakraborty, p. (1995). A text book of microbiology, new center book agency Ltd.India.

Blanco, J. L. and Garcia, M. E. (2008). Immune response to fungal infections. Vet Immunol Immunopathol. 15: 47-70. DOI: https://doi.org/10.1016/j.vetimm.2008.04.020

Mugliston,T. and O’Donoghue, G.( 1985) .“Otomycosis—a continuing problem,” Journal of Laryngology and Otology, 99(4): 327–333. DOI: https://doi.org/10.1017/S002221510009678X

Pontes, Z. B. V. D. S.; Silva, A. D. F.; Lima, E. D. O. et al., “Otomycosis: a retrospective study,” Brazilian Journal of Otorhinolaryngology, vol. 75, no.

Fasunla, J.; Ibekwe, T. and Onakoya, P. (2008). “Otomycosis in western Nigeria,” Mycoses. 51 (1):67–70.

Pradhan, B.; Ratna Tuladhar, N. and Man Amatya, R. (2003) “Prevalence of otomycosis in outpatient department of otolaryngology in Tribhuvan University Teaching Hospital, Kathmandu, Nepal,” Annals of Otology, Rhinology and Laryngology.112(4): 384–387.

Munguia ,R. and Daniel, S. J.( 2008).“Ototopical antifungals and otomycosis: a review,” International Journal of Pediatric Otorhinolaryngology. 72(4): 453–459.

Kaur, R.; Mittal, N.; Kakkar, M.; Aggarwal, A. K. and Mathur, M. D. (2000). “Otomycosis: a clinicomycologic study,” Ear, Nose and Throat Journal, 79 (8): 606–609. DOI: https://doi.org/10.1177/014556130007900815

Viswanatha, B.; Sumatha, D. and Vijayashree, M. S. (2012). “Otomycosis in immunocompetent and immunocompromised patients: comparative study and literature review,” Ear, Nose & Throat Journal, 91: 114–121.

Stern, J. C. and Lucente, F. E. (1988). “Otomycosis,” Ear, Nose and Throat Journal.67 (11): 804–810.

Viswanatha, B. et al. (2012). Otomycosis in immunocompetent and immunocompromised patients; comparative study and literature review, ENT Journal Mar; 91(3):114-21. DOI: https://doi.org/10.1177/014556131209100308

Carney, AS. Otitis externa and otomycosis. In: Gleeson MJj Jones NS, Clarke R, et al. (eds). (2008) Scott-Brown’s Otolaryngology, Head and Neck Surgery, vol 3, 7th edn. London: Hodder Arnold Publishers;3351-7. DOI: https://doi.org/10.1201/b15118-268

Rama Kumar, K. (1984). Silent perforation of tympanic membrane and otomycosis. Indian Journal of Otolaryngology and Head and Neck Surgery.36(4):161-2. DOI: https://doi.org/10.1007/BF02993694

Rutt, A. and Sataloff, R. T. (2008). Aspergillus otomycosis in an immunocompromised patient. ENT J.87(II):622-3.

Abad, JC.; Foster, CS. (1996). Fungal keratitis. Int’l Ophthalmol Clin.36: 1-15. DOI: https://doi.org/10.1097/00004397-199603630-00003

Bennett, J. (2007). Agentes antimicrobianos, agentes antifúngicos. In: Brunton L, editor. Goodman & Gilman: as bases farmacológicas da terapêutica. 11a ed. Rio de Janeiro: McGraw-Hill; c. 1103-17.

William R. Herguth, President, Guy Nadeau.Applications of Scanning Electron Microscopy and Energy Dispersive Spectroscopy (SEM/EDS) To Practical Tribology Problems. Senior Technical Associate Herguth Laboratories, Inc.

Chapin,K.C.and Murray,P.(1999).Media in:Murray,P.R.; Baron ,E.J.;Pflaller ,M.A.;Tenover ,F.C. and Yolken ,R.H.Manual of Clinical Microbiology ,7th Edition .Philadelphia .p:1687-1707.

Kwon-chung, K. J. and Bennett, J. (1992). medical mycology. Leaand Fibiger Philadelphia. London. DOI: https://doi.org/10.1590/S0036-46651992000600018

McGregor,J.A.;French,J.I.and Jone,W. (1992). Association of cervico vaginal infection with increased vaginal fluid phosphates activity .Am.J.Obst Gyn.167:1588-1594. DOI: https://doi.org/10.1016/0002-9378(92)91746-W

Doroghazi and Robert, M. (1981). "Invasive external otitis: report of 21 cases and review of the literature." The American journal of medicine 71.4 :603-614.‏ DOI: https://doi.org/10.1016/0002-9343(81)90213-8

Grandis.;Jennifer Rubin ; Barton, F. ;Branstetter, IV.,and Victor ,L. Yu. (2004). "The changing face of malignant (necrotising) external otitis: clinical, radiological, and anatomic correlations." The Lancet infectious diseases 4.1 .34.‏ DOI: https://doi.org/10.1016/S1473-3099(03)00858-2

Latgé, J. P. (1999). Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 12:310-350. DOI: https://doi.org/10.1128/CMR.12.2.310

Gupta and Smita,. (2007). "Infections in diabetes mellitus and hyperglycemia." Infectious disease clinics of North America. 21:3 617-638.‏ DOI: https://doi.org/10.1016/j.idc.2007.07.003

Gallin, J. I., and Zarember ,K.. (2007). Lessons about the pathogenesis and management of aspergillosis from studies in chronic granulomatous disease. Trans. Am. Clin. Climatol. Assoc. 118:175–185.

Washburn ,R.G. ;Kennedy, DW.; Begley, MG. and Bennett, JE.(1988).Chronic fungal sinusitis in apparently normal hosts.Medicine(Baltimore).67: 231-247. DOI: https://doi.org/10.1097/00005792-198807000-00004

Milory, CM.;Blanshard, J.;Slucas and Michaels, L. (1989).:J clin patho l42:123-127. DOI: https://doi.org/10.1136/jcp.42.2.123

Young, RC.; Bennet, JE. And Vogel, CL.( 1970). Aspergillosis: The spectrum of the disease in 98 patients Medicine(Baltimore). 49: 147-173. DOI: https://doi.org/10.1097/00005792-197003000-00002

Fraser, RS.(1993).Pulmonary aspergillosis: Pathologic and pathogenic features. PatholAnnu. 28: 231-277.

Patterson and Thomas, F. (2016). "Practice guidelines for the diagnosis and management of aspergillosis: 2016 update by the Infectious Diseases Society of America." Clinical Infectious Diseases 63.4: e1-e60.‏ DOI: https://doi.org/10.1093/cid/ciw326

Oh,Y.W.; Effmann, E.L.and Godwin, J.D. (2000). Pulmonary infections in immunocompromised hosts: the importance of correlating the conventional radiologic appearance with the clinical setting. Radiology.217: 647-56. DOI: https://doi.org/10.1148/radiology.217.3.r00dc35647

Vento, S.and Cainelli, F. (2003). Infections in patients with cancer undergoing chemotherapy: aetiology, prevention, and treatment .Lancet. Oncol. 4: 595-604. DOI: https://doi.org/10.1016/S1470-2045(03)01218-X

Lopez, S.; J.B.; Coto, C.D.and Fernandez, C.B. (2002). Neonatal sepsis of nosocomial origin: an epidemiological study from the Grupo de Hospitales Castrillo. J. Perinat. Med.30: 149-157. DOI: https://doi.org/10.1515/JPM.2002.019

Jadhav, VJ.;Pai, M. and Mishra, GS. (2003).Etiological significance of Candida albicans in otitis externa. Mycopathologica.156:313–315. DOI: https://doi.org/10.1023/B:MYCO.0000003574.89032.99

Rutt, AL and Sataloff, RT. (2008). Aspergillus otomycosis in an immunocompromised patient. Ear Nose Throat journal. 87(11):622–623. DOI: https://doi.org/10.1177/014556130808701107

Thrasher, RD. and Kingdom ,TT. (2003).Fungal infections of the head and neck: an update. Otolaryngol Cli N Am.36:577–594. DOI: https://doi.org/10.1016/S0030-6665(03)00029-X

Yassin, A. Maher, A. and Mowad, MK. (1978).Otomycosis: A survey in the eastern province of Saudi Arabia. Journal of laryngology and otology. 92:869–876. DOI: https://doi.org/10.1017/S0022215100086242

Jia, X.; Liang ,Q.; Chi, F. and Cao ,W. (2012). "Otomycosis in Shanghai: aetiology, clinical features and therapy " Mycoses, 55: 404–409. DOI: https://doi.org/10.1111/j.1439-0507.2011.02132.x

Pontes, Z. B. V. D. S. ; Silva, A. D. F ; Lima ,E. D. O and et al.(2009). “Otomycosis: a retrospective study,” Brazilian Journal of Otorhinolaryngology, 75(3): 367–370. DOI: https://doi.org/10.1590/S1808-86942009000300010

Pradhan, B. ; Ratna Tuladhar ,N. and Man Amatya ,R.(2003)."Prevalence of otomycosis in outpatient department of otolaryngology in Tribhuvan University Teaching Hospital, Kathmandu, Nepal,” Annals of Otology, Rhinology and Laryngology, 112(4) :384–387. DOI: https://doi.org/10.1177/000348940311200416

Munguia ,R. and Daniel ,S. J.( 2008) .“Ototopical antifungals and otomycosis: a review,” International Journal of Pediatric Otorhinolaryngology, 72(4) :453–459. DOI: https://doi.org/10.1016/j.ijporl.2007.12.005

Pfaller, MA. and Diekema, DJ. (2004). Rare and emerging opportunistic fungal pathogens: concern for resistance beyond Candida albicans and Aspergillus fumigatus. J Clin Microbiol. 42:4419–31. DOI: https://doi.org/10.1128/JCM.42.10.4419-4431.2004

Klich, M.A. (2002). Identification of common Aspergillus species, CBS, Netherlands.

Rodrigues, P.;Soares, C.; Kozakiewicz1, Z.; Paterson, R.R.M.; Lima, N. and Venâncio, A. (2007). Identification and characterization of Aspergillus flavus and aflatoxins. Communicating Current Research and Educational Topics and Trends in Applied Microbiology (A. Méndez_-Vilas (Ed.): 527-534.

Diba, K.; Kordbacheh, P.; Mirhendi ,S.H.; Rezaie, S. and Mahmoudi,M. (2007). Identification of Aspergillus species using morphological characters. Pak. J. Med. Sci., 23: 867-872. Rev. 12:310–350.

Johnson, EM. and Borman, AM.(2010). The importance of conventional methods: microscopy and culture. In: Pascualotto A, editor. Aspergeukaryotic Cellillosis: from diagnosis to prevention. New York: Springer: 55–73. DOI: https://doi.org/10.1007/978-90-481-2408-4_4

Published

24-04-2022

How to Cite

Jabber, E. Y., Al janabi, O. H. K., & Al-khafaji, Z. A. (2022). Isolation and identification of Aspergillus fumigatus from Immunocompromised patients in AL- Najaf Province. International Journal of Health Sciences, 6(S1), 5689–5703. https://doi.org/10.53730/ijhs.v6nS1.6240

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Peer Review Articles