Molecular detection and Phylogenetic study role of ORF1ab in SARS-COV2 on patients in Hilla city

Noor A. Jawad; Zaytoon A. Al-Khafaji; Bushra J. Al-Tamimi

doi:10.53730/ijhs.v6nS3.6693

Molecular detection and Phylogenetic study role of ORF1ab in SARS-COV2 on patients in Hilla city

https://doi.org/10.53730/ijhs.v6nS3.6693

Authors

Noor A. Jawad
noorabady@vet.uoqasim.edu.iq
Msc student at Department of Microbiology, Babylon University, college of medicine, Iraq
Zaytoon A. Al-Khafaji Department of Microbiology, Babylon university college of medicine, Iraq
Bushra J. Al-Tamimi Department of Microbiology, Babylon university college of medicine, Iraq

Keywords:

ORF1ab, mutation, phylogenetic study

Abstract

The Covid-19 pandemic, which is happening all over the world, needs serious attention. Covid-19 initiated by an RNA type virus can cause more severe problems later on due to its ability to mutate. CoV genomes code for a ORF1a / ORF1ab polyprotein and four structural proteins widely studied as major drug targets. The genomes also contain a variable number of open reading frames (ORFs) coding for accessory proteins that are not essential for virus replication, but appear to have a role in pathogenesis. This study was conducted on 50 nasopharyngeal swaps from Morjan teaching Hospital using RT-PCR and PCR for ORF1ab gene amplification after that, five samples were subjected the phylogenetic analysis. The results are confirmed by the ORF1ab analysis shows that the ORF1ab vary and mutated and have a number of mutation tht could affect it similarity between countries in aimed of detection our local isolate were had a 100 identities with United State concluding that the extreme effect of virus transmission and it is mobile genetic holding.

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Published

27-04-2022

How to Cite

Jawad, N. A., Al-Khafaji, Z. A., & Al-Tamimi, B. J. (2022). Molecular detection and Phylogenetic study role of ORF1ab in SARS-COV2 on patients in Hilla city. International Journal of Health Sciences, 6(S3), 4112–4120. https://doi.org/10.53730/ijhs.v6nS3.6693

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Issue

Vol. 6 No. S3 (2022)

Section

Peer Review Articles

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