Charecterization of mutation induced in Fenugreek by Gamma Ray using rapd marker
Keywords:
Fenugreek, Rapd, Gamma Ray, MutationAbstract
This study was accomplished to detect variation generated through eposure of fenugreek (trigonella foenum-graecum L.) seeds to seven gamma irradiation doses (0,25,50,100,150,300 and 400) GY by using twenty RAPDs primers as molecular marker ,results indicated that primers OPA-14 ,OPC-19 and OPH-01success in giving five treatments a unique fingerprint. Molecular size of amplicon ranged between 2913 bp produced by primer OPB-06 and 118 bp produced by primer OPW-04.Highest degree for polymorphism was 90.92% produced by primer OPC-05. Monomorphic bands appeared in their higher value in primer OPC-08. Primer OPA-15 gave highest number unique bands Primer OPH-01 gave highest value for (amplified, main) bands, discrimination, in addition to primer efficiency value. Silght variation produce among primers OPE-02, OPC-05, OPH-01 and OPA-14. This study revealed RAPD markers in detection mutation induced by gamma irradiation.
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