Charecterization of mutation induced in Fenugreek by Gamma Ray using rapd marker

Taif Razzaq Majeed Najar AL-Saadi; Attyaf Jameel Thamir AL-Tamimi

doi:10.53730/ijhs.v6nS1.6784

Authors

Taif Razzaq Majeed Najar AL-Saadi Department of Biology, Faculty of Science, Kufa University, Iraq and Medical Laboratory Technology Department, College of Medical Technology, The Islamic University, Najaf, Iraq
Attyaf Jameel Thamir AL-Tamimi Department of Biology, Faculty of Science, Kufa University, Iraq

Keywords:

Fenugreek, Rapd, Gamma Ray, Mutation

Abstract

This study was accomplished to detect variation generated through eposure of fenugreek (trigonella foenum-graecum L.) seeds to seven gamma irradiation doses (0,25,50,100,150,300 and 400) GY by using twenty RAPDs primers as molecular marker ,results indicated that primers OPA-14 ,OPC-19 and OPH-01success in giving five treatments a unique fingerprint. Molecular size of amplicon ranged between 2913 bp produced by primer OPB-06 and 118 bp produced by primer OPW-04.Highest degree for polymorphism was 90.92% produced by primer OPC-05. Monomorphic bands appeared in their higher value in primer OPC-08. Primer OPA-15 gave highest number unique bands Primer OPH-01 gave highest value for (amplified, main) bands, discrimination, in addition to primer efficiency value. Silght variation produce among primers OPE-02, OPC-05, OPH-01 and OPA-14. This study revealed RAPD markers in detection mutation induced by gamma irradiation.

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