Mutation induced by gamma irradiation in coriander seeds and their identification by RAPD makers
Keywords:
coriander, RAPD, gamma ray, mutationAbstract
This study was accomplished to detect variation generated through exposure of Coriander ( Coriandrum sativum L.) seeds to five gamma irradiation doses (0,25,50,100 and150 ) GY by using eighteen RAPDs primers as molecular marker ,results indicated that primers OPC-05 ,OPC-19, OPA-10,OPA-15 and OPB-06 success in giving three treatments a unique fingerprint .Molecular size of amplified ranged between 1860 bp produced by primer OPA-04 and 115 bp produced by primer OPW-04 . Highest degree for polymorphism was 91.66 % produced by primer OPA-04. Monomorphic bands appeared in their higher value in primer OPC-09 . Primer OPA-10 gave highest number unique bands ( reached to seven bands ) Primer OPC-19 gave highest value for amplified bands , primer OPA-10 gave highest value for main bands, Discrimination, in addition to primer efficiency value produced in their high value by primer OPA-04.This study revealed RAPD markers in detection mutation induced by gamma irradiation.
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