Beneficial effects of ascorbic acid and calcium in fluoride induced changesin some biochemical parameters in testes of Swiss Albino Mice

Jyoti Sharma; Anita Ranga

doi:10.53730/ijhs.v6nS3.7099

Authors

Jyoti Sharma
Jyoti.sharma0911@gmail.com
Reproductive Biology Laboratory, P.G. Department of Zoology, Govt. Dungar College, Bikaner, Rajasthan, India-334001
Anita Ranga Reproductive Biology Laboratory, P.G. Department of Zoology, Govt. Dungar College, Bikaner, Rajasthan, India-334001

Keywords:

testis, NaF, ascorbic acid, calcium, biochemical changes

Abstract

The present investigation was undertaken to elucidate the effects of sodium fluoride on mice understanding the mechanism of fluoride action on metabolism of testis through sequential biochemical changes in level of different parameters and its reversibility by ascorbic acid or calcium and combined effects of both ascorbic and calcium. The healthy, adult Swiss albino mice (Mus musculus) were divided into two equal groups. Group I (fed on standard diet), Group IIA (NaF 5ppm and 50ppm), Group IIB (withdrawal of treatment), Group II C Recovery was also observed in with simultaneously administration of A.A +Ca respectively. Animals from each group were autopsied by cervical dislocation at each post treatment intervals of 10, 20, and 30 days. The changes in the biochemical parameters were dose and duration dependent. Total protein, acid phosphatase, ascorbic acid DNA and RNA decreased while, alkaline phosphatase, showed an increase in all experimental groups. Whereas after the cessation of the treatment recovery was observed and it was more pronounced with ascorbic acid+ calcium. The experimental findings indicate that both testicular protein and DNA synthesis decreased suggesting that fluoride in some way interfere with the RNA metabolism and consequently interfere with the synthesis of specific testicular enzymes etc.

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References

Thippeswamy HM, Devananda D, Nanditha Kumar M, Wormald MM, & Prashanth, S N, The Association of Fluoride in Drinking Water with Serum Calcium, Vitamin D and Parathyroid Hormone in Pregnant Women and Newborn Infants. Eur. J. Clin. Nutr, 75 (2021) 151–159.

Tian L, Zhu X, Wang L, Peng F & Pang Q, Distribution, Occurrence Mechanisms, and Management of High Fluoride Levels in the Water, Sediment, and Soil of Shahu Lake, China. Appl. Geochem, 126 (2021).

Toolabi A, Bonyadi Z, Paydar M, Najafpoor A A & Ramavandi B, Spatial Distribution, Occurrence, and Health Risk Assessment of Nitrate, Fluoride, and Arsenic in Bam Groundwater Resourc. Iran. Groundwater Sust Dev, (2020)

Kimambo V, Bhattacharya P, Mtalo F, Mtamb J & Ahmad A, Fluoride Occurrence in Groundwater Systems at Global Scale and Status of Defluoridation. State of the Art. Groundwater Sust. Dev, 9 (2019) 100223.

Brindha K & Elango L, Fluoride in Groundwater: Causes, Implications and Mitigation Measures,” in Fluoride Properties, Applications and Environmental Management. Editor S. D. Monroy New York: Nova Publishers, 111–136 (2011)

Brindha K & Elango L, Geochemistry of Fluoride Rich Groundwater in a Weathered Granitic Rock Region, Southern India. Water Qual. Expo. Health, 5 (3) (2013) 127–138.

Vithanage M & Bhattacharya P, Fluoride in the Environment: Sources, Distribution and Defluoridation. Environ. Chem. Lett, 13 (2) (2015) 131–147.

Amini M, Mueller K, Abbaspour KC, Rosenberg T Afyuni M & Møller KN, Statistical Modeling of Global Geogenic Fluorid Contamination in Ground waters. Environ. Sci. Technol, 42 (2008) 3662–3668.

Ali S, Thakur SK, Sarkar A & Shekhar S, Worldwide Contamination of Water by Fluoride. Environ. Chem. Lett, 14 (2008) 291–315.

Bhattacharya P, Samal A C, Banerjee S, Pyne J & Santra SC, Assessment of Potential Health Risk of Fluoride Consumption through rice, Pulses, and Vegetables in Addition to Consumption of Fluoride-Contaminated Drinking Water of West Bengal. India. Environ. Sci. Pollut. Res. 24 (25) (2017) 20300–20314.

Ding Y, Sun H, Han H, Wang W, Ji X & Liu X, The Relationships between Low Levels of Urine Fluoride on Children’s Intelligence, Dental Fluorosis in Endemic Fluorosis Areas in Hulunbuir, Inner Mongolia, China. J. Hazard. Mater. 186 (2-3) (2011) 1942–1946.

Bhattacharya P, Adhikari S, Samal A C, Das, R, Dey D & Deb A, Health Risk Assessment of Co-occurrence of Toxic Fluoride and Arsenic in Groundwater of Dharmanagar Region, Nort. . India. Environ. Sci. Pollut. Res.(2020)

Mridha D, Priyadarshni P, Bhaskar K, Gaurav A, De A, Das, A, Fluoride Exposure and its Potential Health Risk Assessment in Drinking Water and Staple Food in the Population from Fluoride Endemic Regions of Bihar. India, Groundwater Sust. Dev, 13 (2021)

Pal P, Kumar P, Mukhopadhyay Fluoride induced testicular toxicities in adult Wistar rats. Human and exp bio 383-392 (2021).

El-Hallawany HA & El-Metwally AE, Toxopathological studies on the effect of fluoride pollution on male and female fertility in adult rabbits. Assiut Vet Med, J 57 (2011) 376-396.

Sahu SK, Mishra DN, Pradhan S, Prusti JS & Panda SK, Fluoride Induced Histopathological Changes in Liver of Albino Rabbit - An Experimental Study. Int J Pharm Sci Rev Res 30 (2015) 184-188.

Pillai KS, Mathai AT & Deshmukh PB, Acute toxicity of fluoride to mice. Fluoride 20 (1987) 68–70.

Lowry H, Rosen Brough, NJ, Farr, AL & Randall RJ, Protein measurement with folin phenol reagent: J.Bio.Chem, 193 (1951) 265

Fiske CH & Subbarow Y, J. Bio Chem. 66 (1925) 315.

Ceriotti G, J. Bio Chem, 198 (1952) 297.

Roe JH & Kuether CA, Ascorbic acid estimation. J. Biol. Chem, 147 (1943) 399.

Chinoy NJ & Sequeira E, Reversible fluoride induced fertility impairment in male mice. Fluoride, 25(1992) 71.

Trivedi MH, Verma RJ & Chinoy NJ, Black tea amelioration of NaF-induced toxicity in liver and kidney in mice. Fluoride, 41(1) 2008) 61–66.

Sharma JD, Solanki M & Solanki D, NaF Toxicity on Reproductive Organs of Female Albino Rats. Asian J. Exp. Sci, 21(2) (2007) 359-364.

Chinoy NJ & Shah SD, Beneficial effects of some antidotes in fluoride and arsenic inducedtoxicity in kidney of mice. Fluoride, 37(3) (2004) 151-161.

Sharma R, Tsuchiya M, Bartlett JD, Fluorideinduces endoplasmic reticulum stress and inhibits protein synthesis and secretion. Environ Health Perspect, 116(9) (2008).

Rao MV & Chawla SL & Patel N, Melatonin reduction of fluoride nephrotoxicity in mice. Fluoride, 42(2)(2009)110.

Ekambaram P, Namitha T, Bhuvaneswari S, Aruljothi S, Vasanth D & Saravanakumar, M, Effects of tamarind on fluoride induced oxidative stress in the liver of rats. Fluoride, 43(2) (2010) 134.

Chawla SL, Yadav R, Shah D, Rao MV, Protective action of melatonin against fluoride-induced hepatotoxicity in adult female mice. Fluoride, 41(1) (2008) 44.

Rao MV, Chawla SL, & Patel N, Melatonin reduction of fluoride nephrotoxicity in mice. Fluoride, 42(2) (2009) 110.

Tietz NW, Ed Clinical Guide to Laboratory Tests 3rd ed. Philadelphia, Pa: W.B. Saunders (1995) 374.

Banerjee P, Bhattacharyya SS, Bhattacharjee N, Pathak S, Boujedaini N, Belon P, & Khuda-Bukhsh AR, Ascorbic acid combats arsenic-induced oxidative stress in mice liver. Exotoxicol. Environ. Saf, 72 (2009) 639‒649.

Verma R, & Chakraborty D, Emblica officinalis aqueous extract ameliorates ochratoxin-induced lipid peroxidation in thetestis of mice. Acta. Pol. Pharm, 65 (2008) 187–194.

Chinoy NJ & Shah SD, Beneficial effects of some antidotes in fluoride and arsenic induced toxicity in kidney of mice, Fluoride, 37(3) (2004):151.

Chinoy NJ & Shah SD, Beneficial effects of some antidotes in fluoride and arsenic induced toxicity in kidney of mice, Fluoride, 37(3) (2004)151

.Chinoy NJ & Sharma A, Reversal of fluoride-induced alteration in cauda epididymal spermatozoa and fertility impairment in male mice. Environmental Sciences 7(2004) 29.

Ranjan R, Swarup D & Patra RC, Oxidative stress in erythrocytes, liver, and kidneys of F-exposed rabbits. Fluoride, 42(2) (2009/ 88.

Narayana MV& Chinoy NJ, Reversible effect of sodium fluoride ingestion on spermatozoa of the rat. International Journal of Fertility, 39(6) (1994) 337-346.