Beneficial effects of ascorbic acid and calcium in fluoride induced changesin some biochemical parameters in testes of Swiss Albino Mice
Keywords:
testis, NaF, ascorbic acid, calcium, biochemical changesAbstract
The present investigation was undertaken to elucidate the effects of sodium fluoride on mice understanding the mechanism of fluoride action on metabolism of testis through sequential biochemical changes in level of different parameters and its reversibility by ascorbic acid or calcium and combined effects of both ascorbic and calcium. The healthy, adult Swiss albino mice (Mus musculus) were divided into two equal groups. Group I (fed on standard diet), Group IIA (NaF 5ppm and 50ppm), Group IIB (withdrawal of treatment), Group II C Recovery was also observed in with simultaneously administration of A.A +Ca respectively. Animals from each group were autopsied by cervical dislocation at each post treatment intervals of 10, 20, and 30 days. The changes in the biochemical parameters were dose and duration dependent. Total protein, acid phosphatase, ascorbic acid DNA and RNA decreased while, alkaline phosphatase, showed an increase in all experimental groups. Whereas after the cessation of the treatment recovery was observed and it was more pronounced with ascorbic acid+ calcium. The experimental findings indicate that both testicular protein and DNA synthesis decreased suggesting that fluoride in some way interfere with the RNA metabolism and consequently interfere with the synthesis of specific testicular enzymes etc.
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