The cytotoxicity effect of crude aqueous and alcoholic extracts of   Juncus rigidus on human lung cancer cell line (A549) in vitro

Ali Mohmmed Hussen Al-jubouri; Liqaa H. Saqban

doi:10.53730/ijhs.v6nS3.7483

Authors

Ali Mohmmed Hussen Al-jubouri
ali.m.hussein@s.uokerbala.edu.iq
Department of Biology, Collge of Education for Pure Sciences. University of Kerbala, Iraq
Liqaa H. Saqban Department of Biology, Collge of Education for Pure Sciences. University of Kerbala, Iraq

Keywords:

Juncus rigidus, cytotoxcity, inihibition Rate (IR), A549

Abstract

The current study dealt with the identification on cytotoxicity effective of crude water and alcohol extracts from the Juncus rigidus on human lung cancer cells A549. The quantitative estimation of the active compounds of the water and alcohol extracts of the Juncus (Phenols - Flavonoids - Alkaloids - Tannins) was calculated The results of the aqueous extract were (8.82,5.91,13.42,16.29) mg/g, respectively. While the results of the crude alcoholic extract of the plant were (7.01,6.06,13.84,16.34) mg/g, respectively. The cytotoxicity effect of the water and alcohol extracts of the plant was tested on human lung cancer cells A549 and took six concentrations (200,100,50,25,12.5,6.25)micrograms/millilters for 3 exposing times(72, 48, 24)Hr, which recorded highest inhibition rate was for the alcoholic extract of the plant at a concentration (200) µg/ml, for an exposure period (72) hours, and for a probability level (0.05) that reached (83.45%), while (IR) of cancer cells obtained from the aqueous extract was recorded (68.96%).

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