Cytotoxic effect of the crude alcoholic extract of Juncus rigidus on cells of the human breast cancer line MCF-7 in vitro
Keywords:
Juncus rigidus, Cytotoxcity, DPPH, MCF-7Abstract
This study tested the toxicological activity of the crude alcoholic extract of the vegetative part of Juncus rigidus on cells of the MCF-7. The quantitative estimation of the active compounds of the alcoholic extract (alkaloids - tannins - flavonoids - phenols) was found, as the results gave (6.06 - 7.91 - 13.84 - 16.34 mg/g) respectively. The antioxidant activity of the alcoholic extract was also calculated by DPPH method. The percentages of free radical inhibition were (9.31%, 16.43%, 19.81%,20.51%,24.31%) at concentrations (10,20,30,40,50 mg/ml) respectively. Six concentrations of (6.25, 12.5, 25, 50, 100, 200 μg/ml) of alcoholic extract of the vegetative part of the plant were tested on the growth of human breast cancer cells MCF-7 line for ( 3 ) exposure periods (24,48,72) Hr .Where the extract had a toxic effect on cell growth from the lowest used concentration (6.25 μg/ml), where the inhibition percentage was recorded (2±32.8%) for the 72-hour exposure period and for a probability level (P≤ 0.05) versus (72.3%±2) at the concentration (200 µg/ml) and for the same exposure time and probability level.
Downloads
References
Sekar, P., Ravitchandirane, R., Khanam, S., Muniraj, N., & Cassinadane, A. V. (2022). Novel molecules as the emerging trends in cancer treatment: an update. Medical Oncology, 39(2), 1-24.
Mohmoud S.A. AL-Mounasi & Fatin A-J- Mustafa.(2009). Protoscolicidal activity of aqueous and alcoholic extracts of Juncus rigidus (Junceceae) in vitro. Basrah Technical institutes, Bas.J.Vet.Res.Vol.8,No.1.
El-Shamy, A. I., Abdel-Razek, A. F., & Nassar, M. I. (2015). Phytochemical review of Juncus L. genus (Fam. Juncaceae). Arabian Journal of Chemistry, 8(5), 614-623.
Ma, W., Liu, F., Ding, Y. Y., Zhang, Y., & Li, N. (2015). Four new phenanthrenoid dimers from Juncus effusus L. with cytotoxic and anti-inflammatory activities. Fitoterapia, 105, 83-88.
Qi, J., Jiang, W., Chen, G., Zhou, D., & Li, N. (2020). Studies on the chemical components and biological activities of the genus of Juncus. Asian Journal of Traditional Medicines, 15(6).
Chadha, Y. (1976). The wealth of India. A dictionary of indian raw materials and industrial products, 11, 112-122.
Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of the university of chemical technology and metallurgy. 2005 Jul;40(3):255-60.
Ajanal M, Gundkalle MB, Nayak SU. Estimation of total alkaloid in Chitrakadivati by UV-Spectrophotometer. Ancient science of life. 2012 Apr;31(4):198.
Paśko P, Tyszka-Czochara M, Trojan S, Bobis-Wozowicz S, Zagrodzki P, Namieśnik J, Haruenkit R, Poovarodom S, Pinsirodom P, Gorinstein S. Glycolytic genes expression, proapoptotic potential in relation to the total content of bioactive compounds in durian fruits. Food Research International. 2019 Nov 1;125:108563.
Broadhurst RB, Jones WT. Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture. 1978 Sep;29(9):788 94.
Marinova G, Batchvarov V. Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science. 2011 Feb 1;17(1):11-24.
Mansoori, B., Mohammadi, A., Davudian, S., Shirjang, S., & Baradaran, B. (2017). The different mechanisms of cancer drug resistance: a brief review. Advanced pharmaceutical bulletin, 7(3), 339.
Su, X. H., Yuan, Z. P., Li, C. Y., Zhong, Y. J., Du, H. J., Wen, Y. Y., ... & Liang, B. (2013). Phenanthrenes from Juncus effusus. Planta medica, 79(15), 1447-1452.
Platella, C., Capasso, D., Riccardi, C., Musumeci, D., DellaGreca, M., & Montesarchio, D. (2021). Natural compounds from Juncus plants interacting with telomeric and oncogene G-quadruplex structures as potential anticancer agents. Organic & Biomolecular Chemistry, 19(45), 9953-9965.
Wada, A., Murakami, K., Ishikawa, Y., Amoh, T., Hirao, K., Hosokawa, Y., ... & Yumoto, H. (2022). Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes. BioMed Research International, 2022.
Hordyjewska, A., Ostapiuk, A., Horecka, A., & Kurzepa, J. (2019). Betulin and betulinic acid: Triterpenoids derivatives with a powerful biological potential. Phytochemistry Reviews, 18(3), 929-951.
Wada, A., Murakami, K., Ishikawa, Y., Amoh, T., Hirao, K., Hosokawa, Y., ... & Yumoto, H. (2022). Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes. BioMed Research International,2022.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
