Isoltation of Staphylococcus aureus from chicken and study sensitivity to antibiotic
Keywords:
Staphylococcus aureus, chicken, antibioticsAbstract
Aim: This study was designed to determine the prevalence of Staphylococcus aureus in fresh and frozen chicken and study sensitivity of bacterial isolates to antibiotics Methods: A total of (100) liver samples were collected from different market in Baghdad city during period from (10/2020-2/ 2021). The samples were subjected for isolation of following standard bacteriological examinations such as cultural and morphological characteristics, biochemical properties, antibiotics sensitivity (Vancomycin 30 mg, Tetracycline 10mg, Penicilin 10U, Gentamicin 10mg, Amoxicillin 25 mg, Ampicillin 25 mg,Trimethoprim/Sulphamethaxazole 25mg, Streptomycin 25 mg). Results: the results showed that 30 / 100 (30%) of Staphylococcus aureus isolates, 10/50 (20%) for fresh and 20/50 (40%) for frozen The growth of colony with different characteristics biochemical test indicated positive results, the results showed that Staphylococcus aureus isolates were senstitive (90%) to Gentamicin and Amoxicillin (80%) to Streptomycinand Vancomycin, (70%) to Trimethoprim/Sulphamethaxazole, (10%) to Ampicillin, and Resistant (90%) to Penicilin (80%) to Ampicillin (60%) to Tetracycline (20%) to Trimethoprim/Sulphamethaxazole (10%) Vancomycin, Gentamicin, Streptomycin.
Downloads
References
Gotz. The intercellular adhesion (ica) locus in Staphylococcus aureus and is required for biofilm formation. Infect. Immun. (1999) 67(10): 5427-5433
pottumarthy S, Schapiro JM, Prentice JL, Houze YB, Swanzy SR, Fang FC, Cookson BT Clinical isolates of Staphylococcus intermedius masquerading as methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2004 42: 5881–5884)
Ortega E, Abriouel H, Lucas R, Gálvez A: Multiple roles of Staphylococcus aureus enterotoxins: pathogenicity, superantigenic activity, and correlation to antibiotic resistance. Toxins (2010)2: 2117–2131)
Devriese LA, Schleifer KH, Adegoke GO Identification of coagulase-negative staphylococci from farm animals. J Appl Microbiol 1985 58: 45–55).
Varrone JJ, de Mesy Bentley KL, Bello-Irizarry SN, Nishitani K, Mack S, Hunter JG, Kates SL, Daiss JL, Schwarz EM. "Passive immunization with anti-glucosaminidase monoclonal antibodies protects mice from implant-associated osteomyelitis by mediating opsonophagocytosis of Staphylococcus aureus megaclusters". JournalofOrthopaedicResearch. 2014 32 (10):138996. doi:10.1002/jor.22672. PMC 4234088. PMID 24992290
Sampimon OC, Zadoks RN, De Vliegher S, Supre K, Haesebrouck F, Barkema HW, Sol J, Lam TJGM: Performance of API Staph ID 32 and Staph-Zym for identification of coagulase-negative staphylococci isolated from bovine milk samples. Vet Microbiol 2009 136: 300–305
Quinn, J., Carter, E., Markey, B. and Carter, R.: Clinical Veterinary Microbiology. Mosby: London, UK., Pp. 2000 21-66
Murray, P.R. Manuel of clinical microbiology. 8th ed. Washington D.C: ASM2003
Naas, H.T., Garbaj, A.M., Elarbed, Z.M., Eshamah, H.L and Abolghit, S.K. Microbial status of fresh beef sausage sold in Tripoli. Suez Canal Vet. Med. J., 2009b 14(2): 111-118
Baba-Moussa L, Ahissou H, Azokpota P, Assogba B, AtindéhouMM, Anagonou S, Keller D, Sanni A, Prévost G .Toxinsand adhesion factors associated with Staphylococcus aureus strains isolated from diarrhealpatient in Benin. Afr J Biotechnol 2010 9:604–611 .
Zakki SA, Qureshi R, Hussain A, Ghias W, Sharif M, Ansari F. Microbial Quality Evaluation and Prevalence of Bacteria and Fungus in Different Varieties of Chicken Meat in Lahore. J. Pharmac. Pharma. Sci. 2017 5 (1): 30-37.
Al-Hemairi MA .Study the incidence of bacterial and fungal contaminations in frozen poultry livers. Al-Qadisiya J. Pure Scie. 201116 (4): 1-9.
Easa SMH. The Microbial Quality of Fast Food and Traditional Fast Food. Natur. Sci. (2010 8 (10): 117-133.
Kitai, S.; Shimizu, A.; Kawano, J.; Sato, E.; Nakano, C.; Uji,T.;Kitagawa,HCharacterization of methicillin-resistant Staphylococcus aureus isolated from retail raw chicken meat in Japan. J. Vet.Med. Sci. 2005 67, 107–110
Abu-Salem FM, Abou-Arab EA. Chemical properties,microbiological quality and sensory evaluation of chicken and duck liver paste (foie gras). Grasas Y. Aceites. 2010 61 (2): 126-135
Abd El-Tawab, A.A.., Fahmy, H.A., El Hofy, E.I.Elshawadfy, A.A.. Detection of species-specific gene thermonuclease (nuc) in Staphylococcus aureus from chickens by real- time PCR assay. Benha Vet.Med.J. 2014 27(2):415‐422.
Onaolapo J0A, Igwe JC, Bolaji RO, Adeshina GO, Parom SK. Antibiotics Susceptibility Profile of Staphylococcus aureus Isolated from Poultry Birds in Kaduna, Nigeria. J Clin Microbiol Antimicrob 20171: 101
Nworie, A., Elom, MO., Ibiam, AG. Simon, OA. Simon,IO. Multi-drug resistant Staphylococcus aureus from poultry farms in Ebonyi state, Nigeria. Int. J. Micro.Biol. Genet Monocular Biol. Res. 2016 2:1-11.
Rahimi, E., Nonahal, F. and Salehi, E.A. Detection of classical enterotoxins of Staphylococcus aureus strains isolated from raw meat in Esfahan, Iran. Health Scope, 20132(2):95-98).
Kechih, B.S., Hamdi,M.T., Aggad, H., Meguenni, N.,Cantekin, Z. Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria. Hindawi Vet. Med. Inter. 5 pages2018
Nasef, A.S., El Oksh, S.A., Ibrahim, A.G., Eid, SCorrelation between antibiotic resistance genes and virulence genes carried on mobile genetic elements of staphylococci isolated from broilers. Anim. Health Res.J. 20175 (4):389-401.
Mamza, S. A., Egwu,G.O., Mshelia,G.D. Antibiotic susceptibility patterns of beta-lactamase-producing Escherichia coli and Staphylococcus aureus isolated from chickens in Maiduguri (Arid zone), Nigeria. Vet.Archive 201080(2): 283-297.
Badr, H., El-Enbaawy, M.A., Nasef, S.A Incidence of coagulase positive Staphylococcus aureus in poultry and related human. 1st Conf. of An. Health Res. Inst Assoc. 2012333 – 340.
Olsen, R.H., Christensen, H., Kabell, S., Bisgaard, M. Characterization of prevalent bacterial pathogens associated with pododermatitis in table egg layers J.Avian Path2018.47(3) :281-285
LNCSM. Libyan National Center for Standardization and Metrology. LNCSM 600-2009. LNCSM, Libya
Kadariya, J., Smith, T.C. and Thapaliya, D. Staphylococcus aureus and staphylococcal food-borne disease: An ongoing challenge in public health. Biomed. Res. Int., 2014: 827965
Kelman, A.; Soong, Y.A.; Dupuy, N.; Shafer, D.; Richbourg, W.; Johnson, K.; Meng, J.Antimicrobial susceptibility of Staphylococcus aureus from retail ground meats. J. FoodProt74,2011 1625–1629.
Goja, A.M., Ahmed, T.A.A., Saeed, S.A.M. and Dirar, H.A. Isolation and identification of Staphylococcus spp . in fresh beef. Pak. J. Nutr., 2013 12(2): 114-120).
Greeson, K., Suliman, G.M., Sami, A., Alowaimer, A. and Koohmaraie, M. Frequency of antibiotic-resistant Salmonella, Escherichia coli, Enterococcus, and Staphylococcus aureus in meat in Saudi Arabia. Afr. J. Microbiol. Res., 2013 7(4): 309-316
Badr*, Nayera M. AlAtfeehyand Soad A. Nasef. Detection of food borne pathogens from retail chicken Laboratory for Veterinary Quality Control on Poultry(BVMJ‐201631(2):276‐282
Al-Jobori, A. H. (2021). DETECTION OF BACTERIOCIN (STAPHYLOCOCCIN) ACTIVITY OF STAPHYLOCOCCUS AUREUS ISOLATES IN CHICKENS. The Iraqi Journal of Veterinary Medicine, 26(1), 165–168. https://doi.org/10.30539/ijvm.v26i1.1136
Aarestrup FM, Agersal Y, Ahrens P, Jirgensen JC, Madsen M, Jensen LB: Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry. Vet Microbiol 2000 74: 353–364.
Wieliczko A, Król J, Piasecki T, Mazurkiewicz M, Staroniewicz Z: Occurrence and characteristics of Staphylococci isolated from poultry. Med Weter2002 58: 348–352).
Buyukcangaz, E.; Velasco, V.; Sherwood, J.S.; Stepan, R.M.; Koslofsky, R.J.; Logue, C.M. Molecular typing of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) isolated from animals and retail meat in North Dakota, United States. Foodborne Pathog. Dis. 2013 10, 608–617.
Gundogan, N.; Citak, S.; Yucel, N; Devren, Y.A. A. note on the incidence and antibiotic resistance of Staphylococcus aureus isolated from meat and chicken samples. Meat Sci 200569, 807–810).
Zeitoun, A. A. and Al-Eid, S. M. The safety and quality of frozen poultry at 2003
Al-Hassa supermarkets. Sitted in, www.Kfu.edu.sa/Main/res/3014-Pdf Int .J. Curr. Microbiol.App.Sci,(2018),7(2):,867-872,867.
Abdalrahman LS, Stanley A, Wells H, Fakhr MK. Isolation,virulence, and antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA) strains from Oklahoma retail poultry meats. International Journal of Environmental Research and Public Health. 2015 12 (6); 6148-6161)
Boost, M., Ho, J., Guardabassi, L. and O’Donoghue, M Colonization of butchers with livestock-associated Methicillin-resistant Staphylococcus aureus. Zoonose Public Health, 201360(8): 572-576
Kechih, B.S., Hamdi,M.T., Aggad, H., Meguenni, N.,Cantekin, Z. Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria. Hindawi Vet. Med. Inter. 5 pages2018
Abd El-Tawab, A.A., Hofy, F.I., Mohamed, S.R. AminS.H. Characterization of Methicillin Resistance staphylococcus aureus isolated from chicken and human.Benha Vet. Med. J. 201732 (1):132 ‐137.
Onaolapo J0A, Igwe JC, Bolaji RO, Adeshina GO, Parom SK Antibiotics Susceptibility Profile of Staphylococcus aureus Isolated from Poultry Birds in Kaduna, Nigeria. J Clin Microbiol Antimicrob2017 1: 101
Rahimi, E., Nonahal, F. and Salehi, E.A. Detection of classical enterotoxins of Staphylococcus aureus strains isolated from raw meat in Esfahan, Iran. Health Scope, 20132(2):95-98).
Nworie, A., Elom, MO., Ibiam, AG. Simon, OA. Simon,IO. Multi-drug resistant Staphylococcus aureus from poultry farms in Ebonyi state, Nigeria. Int. J. Micro.Biol. Genet Monocular Biol. Res. 20162:1-11.
El Bayomi, R.M., Ahmed, H.A., Awadallah, M.A.I., Mohsen, R.A., Abd El-Ghafar, A.E. , Abdelrahman, M.A.. Occurrence, Virulence Factors, Antimicrobial Resistance,and Genotyping of Staphylococcus aureus Strains Isolated from Chicken Products and Humans. vector- borne and zoonotic dis. 201616(3):157-164
Bywater R, Deluyker H, Deroover E, de Jong A, Martion H, McConville M, Rowan T, Shryock T, Shuster D, Thomas V, Valle M, Walters J: A European survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing animals. J Antimicrob Chemother 200454: 744–754
Van Loo IH, Diederen BM, Savelkoul PH, Woudenberg JH, Roosendaal R, VanBelkum A, Lemmens-den Toom N, Verhulst C, Van Keulen PH, Kluytmans JA. Methicillin- resistant Staphylococcus aureus in meat products, the Netherlands. Emerging infectious diseases. 200713 (11); 1753.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
