Role of IL-23 gene expression in development of psoriatic arthritis among psoriasis patients

Zainab Sabeeh Al-Hwas; Naael H Ali; Khalel Ismael Al-Hamdi; Zainab A Mahmood

doi:10.53730/ijhs.v6nS2.8797

Authors

Zainab Sabeeh Al-Hwas
zainabsabeeb@gmail.com
College of Pharmacy, University of Basrah, Basrah, Iraq
Naael H Ali College of Pharmacy, University of Basrah, Basrah, Iraq
Khalel Ismael Al-Hamdi College of Medicine, University of Basrah, Basrah, Iraq
Zainab A Mahmood College of Medicine, University of Basrah, Basrah, Iraq

Keywords:

psoriatic arthritis, IL-23, real time, PCR, gene expression

Abstract

Background: Psoriasis (PS) is an inflammatory skin disorder that lasts for a long time. About 30% of peoples with psoriatic arthritis and characterized as inflammatory arthritis that affects joints or entheses. Although there is growing evidence that interleukin-23 is a kind of interleukin that is produced by (IL-23) Signaling is important in the pathophysiology of both PS and PsA, it is unclear whether IL-23-induced skin inflammation causes joint disease. The goal of this research is to determine the expression of genes (IL-23) in PsA patients in Basrah. Material and Methods: The current study included 88 participants, 59 of whom had Poraisis (Ps), 29 of whom had Psoriatic arthritis, and 40 healthy controls (HC). From August 2020 to February 2022, samples were collected from patients at Al-Sadar Teaching Hospital and Basrah Teaching Hospital's Rheumatology Unite and Biological Therapy Center. Total RNA was collected from peripheral blood and used to evaluate the expression of the genes of interest using qRT-PCR. Results: The binding of the SYBR green dye was unique to the target genes through one peak in the melting curve data and amplification curve for the genes of interest, which included IL-23.

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References

Alobaidi, A. H., Mothana, Z., Najem, W. S., Alsamarai, A. M. J. A. J. o. D., & Venereology. (2012). Adiponectin, IL-10, IL-23 and trace elements serum levels in patients with psoriasis. 1(2), 6-23.

Bai, F., Zheng, W., Dong, Y., Wang, J., Garstka, M. A., Li, R., . . . Ma, H. J. O. (2018). Serum levels of adipokines and cytokines in psoriasis patients: a systematic review and meta-analysis. 9(1), 1266.

Bilgiç, Ö., Sivrikaya, A., Toker, A., Ünlü, A., & Altınyazar, C. J. C. (2016). Serum levels of TWEAK in patients with psoriasis vulgaris. 77, 10-13.

Boutet, M.-A., Nerviani, A., Gallo Afflitto, G., & Pitzalis, C. J. I. j. o. m. s. (2018). Role of the IL-23/IL-17 axis in psoriasis and psoriatic arthritis: the clinical importance of its divergence in skin and joints. 19(2), 530.

Bridgewood, C., Sharif, K., Sherlock, J., Watad, A., & McGonagle, D. J. I. r. (2020). Interleukin‐23 pathway at the enthesis: The emerging story of enthesitis in spondyloarthropathy. 294(1), 27-47.

Chen, L., Deshpande, M., Grisotto, M., Smaldini, P., Garcia, R., He, Z., . . . Furtado, G. C. J. S. R. (2020). Skin expression of IL-23 drives the development of psoriasis and psoriatic arthritis in mice. 10(1), 1-11.

Chervoneva, I., Li, Y., Schulz, S., Croker, S., Wilson, C., Waldman, S. A., & Hyslop, T. J. B. b. (2010). Selection of optimal reference genes for normalization in quantitative RT-PCR. 11(1), 1-15.

Coates, L. C., Kavanaugh, A., Mease, P. J., Soriano, E. R., Laura Acosta‐Felquer, M., Armstrong, A. W., . . . rheumatology. (2016). Group for research and assessment of psoriasis and psoriatic arthritis 2015 treatment recommendations for psoriatic arthritis. 68(5), 1060-1071.

Deodhar, A., Helliwell, P. S., Boehncke, W.-H., Kollmeier, A. P., Hsia, E. C., Subramanian, R. A., . . . Zhou, B. J. T. L. (2020). Guselkumab in patients with active psoriatic arthritis who were biologic-naive or had previously received TNFα inhibitor treatment (DISCOVER-1): a double-blind, randomised, placebo-controlled phase 3 trial. 395(10230), 1115-1125.

Fleige, S., & Pfaffl, M. W. J. M. a. o. m. (2006). RNA integrity and the effect on the real-time qRT-PCR performance. 27(2-3), 126-139.

Generali, E., Ceribelli, A., Stazi, M. A., & Selmi, C. J. J. o. a. (2017). Lessons learned from twins in autoimmune and chronic inflammatory diseases. 83, 51-61.

Gooderham, M., Papp, K., Lynde, C. J. J. o. t. E. A. o. D., & Venereology. (2018). Shifting the focus–the primary role of IL‐23 in psoriasis and other inflammatory disorders. 32(7), 1111-1119.

Hawkes, J. E., Yan, B. Y., Chan, T. C., & Krueger, J. G. J. T. J. o. I. (2018). Discovery of the IL-23/IL-17 signaling pathway and the treatment of psoriasis. 201(6), 1605-1613.

Josko, D. J. C. L. S. (2010). Molecular virology in the clinical laboratory. 23(4), 231.

Khodadadi, A., Razmkhah, M., Eskandari, AR., Hosseini, A., Habibagahi, M., Ghaderi, A. and Jberipour, M. (2014). IL-23/IL-17 ratio in peripheral blood of patients with breast cancer. Iran. J. Med. Sci, 350-356.

Mease, P. J., McInnes, I. B., Kirkham, B., Kavanaugh, A., Rahman, P., Van Der Heijde, D., . . . Yuan, J. J. N. E. J. o. M. (2015). Secukinumab inhibition of interleukin-17A in patients with psoriatic arthritis. 373(14), 1329-1339.

Mease, P. J., Rahman, P., Gottlieb, A. B., Kollmeier, A. P., Hsia, E. C., Xu, X. L., . . . Zhuang, Y. J. T. L. (2020). Guselkumab in biologic-naive patients with active psoriatic arthritis (DISCOVER-2): a double-blind, randomised, placebo-controlled phase 3 trial. 395(10230), 1126-1136.

Michalak-Stoma, A., Bartosińska, J., Kowal, M., Juszkiewicz-Borowiec, M., Gerkowicz, A., & Chodorowska, G. J. D. m. (2013). Serum levels of selected Th17 and Th22 cytokines in psoriatic patients. 35(6), 625-631.

Nerviani, A., Boutet, M.-A., Tan, W. S. G., Goldmann, K., Purkayastha, N., Lajtos, T. A., . . . Pitzalis, C. J. A. o. t. r. d. (2021). IL-23 skin and joint profiling in psoriatic arthritis: novel perspectives in understanding clinical responses to IL-23 inhibitors. 80(5), 591-597.

Oliveira, M. d. F. S. P. d., Rocha, B. d. O., & Duarte, G. V. J. A. b. d. d. (2015). Psoriasis: classical and emerging comorbidities. 90, 9-20.

Olivieri, I., Padula, A., D'ANGELO, S., & Cutro, M. S. J. T. J. o. R. S. (2009). Psoriatic arthritis sine psoriasis. 83, 28-29.

Pujadas, E., Chaudhry, F., McBride, R., Richter, F., Zhao, S., Wajnberg, A., . . . Cordon-Cardo, C. J. T. L. R. M. (2020). SARS-CoV-2 viral load predicts COVID-19 mortality. 8(9), e70.

Ramiro, S., Smolen, J. S., Landewé, R., van der Heijde, D., Dougados, M., Emery, P., . . . Gossec, L. J. A. o. t. r. d. (2016). Pharmacological treatment of psoriatic arthritis: a systematic literature review for the 2015 update of the EULAR recommendations for the management of psoriatic arthritis. 75(3), 490-498.

Ritchlin, C., Rahman, P., Kavanaugh, A., McInnes, I. B., Puig, L., Li, S., . . . Mendelsohn, A. M. J. A. o. t. r. d. (2014). Efficacy and safety of the anti-IL-12/23 p40 monoclonal antibody, ustekinumab, in patients with active psoriatic arthritis despite conventional non-biological and biological anti-tumour necrosis factor therapy: 6-month and 1-year results of the phase 3, multicentre, double-blind, placebo-controlled, randomised PSUMMIT 2 trial. 73(6), 990-999.

Ritchlin, C. T., Colbert, R. A., & Gladman, D. D. J. N. E. J. o. M. (2017). Psoriatic arthritis. 376(10), 957-970.

Rodríguez-Lázaro, D., & Hernández, M. J. C. i. i. m. b. (2013). Real-time PCR in food science: introduction. 15(2), 25-38.

Scher, J. U., Ogdie, A., Merola, J. F., & Ritchlin, C. J. N. R. R. (2019). Preventing psoriatic arthritis: focusing on patients with psoriasis at increased risk of transition. 15(3), 153-166.

Tajadini, M., Panjehpour, M., & Javanmard, S. H. J. A. b. r. (2014). Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes. 3.

Valasek, M. A., & Repa, J. J. J. A. i. p. e. (2005). The power of real-time PCR. 29(3), 151-159.

Valente, T. W. J. s. (2012). Network interventions. 337(6090), 49-53.

Widana, I.K., Sumetri, N.W., Sutapa, I.K., Suryasa, W. (2021). Anthropometric measures for better cardiovascular and musculoskeletal health. Computer Applications in Engineering Education, 29(3), 550–561. https://doi.org/10.1002/cae.22202

Zabotti, A., Tinazzi, I., Aydin, S. Z., & McGonagle, D. J. C. R. R. (2020). From psoriasis to psoriatic arthritis: insights from imaging on the transition to psoriatic arthritis and implications for arthritis prevention. 22(6), 1-9.