A distinct inflammasome IL-1β gene expression profile in patients with psoriatic arthritis in Basra city

Zainab Sabeeh Al-Hwas; Naael H Ali; Khalel I Al-Hamdi

doi:10.53730/ijhs.v6nS4.9122

Authors

Zainab Sabeeh Al-Hwas College of Pharmacy, University of Basrah, Basrah, Iraq
Naael H Ali
Naael.ali@uobasrah.edu.iq
College of Pharmacy, University of Basrah, Basrah, Iraq
Khalel I Al-Hamdi College of Medicine, University of Basrah, Basrah, Iraq

Keywords:

psoriatic arthritis, IL-1β, psoriasis, gene expression

Abstract

Psoriatic arthritis (PsA) is defined as a heterogeneous inflammation correlating with psoriasis, usually sero-negative inflammatory arthropathy and the factor genes essential for its expression and prediction. Therefore, current study aimed to determination whether IL-1β gene expression has a role in the development of psoriatic arthritis. A total of (128) individual participated in this study, (59) with psoriasis, and (29) others with psoriatic arthritis, in addition to (40) healthy subjects. All participants were from those who attended Al-Sadr Teaching Hospital or Al-Basra teaching hospital in Basra governorate, southern Iraq, from August 2020 to February 2022. After the samples were collected, peripheral blood was isolated and total RNA was extracted to estimate the expression of the genes of interest using q RT-PCR. Our results confirmed that the expression level of IL-1β in the PsA patients was up-regulated by 22.09-fold compared with the down-regulated psoriasis patients by 2.8. Thus, we concluded that IL-1β was significant in the development of psoriatic arthritis.

Downloads

Download data is not yet available.

References

Asriyati, P. E., Swarjana, I. K., Sastriani, N. L. A., & Krisnandari, A. A. I. W. (2021). The effect of electronic discharge planning with SBAR approach to optimize the implementation of patient discharge. International Journal of Health & Medical Sciences, 4(3), 280-287. https://doi.org/10.31295/ijhms.v4n3.1750

Bhat, I. A., Naykoo, N. A., Qasim, I., Ganie, F. A., Yousuf, Q., Bhat, B. A., . . . Shah, Z. A. (2014). Association of interleukin 1 beta (IL-1β) polymorphism with mRNA expression and risk of non small cell lung cancer. Meta gene, 2, 123-133.

Broz, P., & Dixit, V. M. (2016). Inflammasomes: mechanism of assembly, regulation and signalling. Nature Reviews Immunology, 16(7), 407-420.

Chua-Aguilera, C. J., Möller, B., & Yawalkar, N. (2017). Skin manifestations of rheumatoid arthritis, juvenile idiopathic arthritis, and spondyloarthritides. Clinical reviews in allergy & immunology, 53(3), 371-393.

Gu, J., Märker‐Hermann, E., Baeten, D., Tsai, W., Gladman, D., Xiong, M., . . . Song, Y. (2002). A 588‐gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients. Rheumatology, 41(7), 759-766.

Juneblad, K., Kastbom, A., Johansson, L., Rantapää-Dahlqvist, S., Söderkvist, P., & Alenius, G.-M. (2021). Association between inflammasome-related polymorphisms and psoriatic arthritis. Scandinavian Journal of Rheumatology, 50(3), 206-212.

Levescot, A., Chang, M. H., Schnell, J., Nelson-Maney, N., Yan, J., Martínez-Bonet, M., . . . Blaustein, R. B. (2021). IL-1β–driven osteoclastogenic Tregs accelerate bone erosion in arthritis. The Journal of Clinical Investigation, 131(18).

Livshits, G., & Kalinkovich, A. (2018). Hierarchical, imbalanced pro-inflammatory cytokine networks govern the pathogenesis of chronic arthropathies. Osteoarthritis and Cartilage, 26(1), 7-17.

Man, S. M., Karki, R., & Kanneganti, T. D. (2016). AIM2 inflammasome in infection, cancer, and autoimmunity: Role in DNA sensing, inflammation, and innate immunity. European journal of immunology, 46(2), 269-280.

Mease, P. J., Bhutani, M. K., Hass, S., Yi, E., Hur, P., & Kim, N. (2021). Comparison of clinical manifestations in rheumatoid arthritis vs. spondyloarthritis: a systematic literature review. Rheumatology and Therapy, 1-48.

Meizlish, M. L., Franklin, R. A., Zhou, X., & Medzhitov, R. (2021). Tissue homeostasis and inflammation. Annual review of immunology. 557-581.

Monnet, D., Kadi, A., Izac, B., Lebrun, N., Letourneur, F., Zinovieva, E., . . . Breban, M. (2012). Association between the IL-1 family gene cluster and spondyloarthritis. Annals of the rheumatic diseases, 71(6), 885-890.

Netea, M. G., Simon, A., van de Veerdonk, F., Kullberg, B.-J., Van der Meer, J. W., & Joosten, L. A. (2010). IL-1β processing in host defense: beyond the inflammasomes. PLoS pathogens, 6(2), e1000661.

Nich, C., Takakubo, Y., Pajarinen, J., Ainola, M., Salem, A., Sillat, T., . . . Takagi, M. (2013). Macrophages—key cells in the response to wear debris from joint replacements. Journal of biomedical materials research Part A, 101(10), 3033-3045.

Nyandra, M., Kartiko, B.H., Susanto, P.C., Supriyati, A., Suryasa, W. (2018). Education and training improve quality of life and decrease depression score in elderly population. Eurasian Journal of Analytical Chemistry, 13(2), 371-377.

Próchnicki, T., & Latz, E. (2017). Inflammasomes on the crossroads of innate immune recognition and metabolic control. Cell metabolism, 26(1), 71-93.

Rabolli, V., Lison, D., & Huaux, F. (2015). The complex cascade of cellular events governing inflammasome activation and IL-1β processing in response to inhaled particles. Particle and Fibre Toxicology, 13(1), 1-17.

Ranjbaran, R., Okhovat, M. A., Mobarhanfard, A., Aboualizadeh, F., Abbasi, M., Moezzi, L., . . . Sharifzadeh, S. (2013). Analysis of β/α globin ratio by using relative qRT‐PCR for diagnosis of beta‐thalassemia carriers. Journal of clinical laboratory analysis, 27(4), 267-271.

Ravindran, J., Owen, P., Lagan, A., Lewis, J., Korendowych, E., Welsh, K., & McHugh, N. (2004). Interleukin 1α, interleukin 1β and interleukin 1 receptor gene polymorphisms in psoriatic arthritis. Rheumatology, 43(1), 22-26.

Saalfeld, W., Mixon, A. M., Zelie, J., & Lydon, E. J. (2021). Differentiating Psoriatic Arthritis from Osteoarthritis and Rheumatoid Arthritis: A Narrative Review and Guide for Advanced Practice Providers. Rheumatology and Therapy, 8(4), 1493-1517.

Satoh, T., Otsuka, A., Contassot, E., & French, L. E. (2015). The inflammasome and IL-1β: implications for the treatment of inflammatory diseases. Immunotherapy, 7(3), 243-254.

Schett, G., Rahman, P., Ritchlin, C., McInnes, I. B., Elewaut, D., & Scher, J. U. (2022). Psoriatic arthritis from a mechanistic perspective. Nature Reviews Rheumatology, 18(6), 311-325.

Son, C.-N., Bang, S.-Y., Kim, J. H., Choi, C.-B., Kim, T.-H., & Jun, J.-B. (2013). Caspase-1 level in synovial fluid is high in patients with spondyloarthropathy but not in patients with gout. Journal of Korean Medical Science, 28(9), 1289-1292.

Spel, L., & Martinon, F. (2020). Inflammasomes contributing to inflammation in arthritis. Immunological reviews, 294(1), 48-62.

Stehlik, C. (2009). Multiple IL-1β converting enzymes contribute to inflammatory arthritis. Arthritis and rheumatism, 60(12), 3524.

Stober, C. (2021). Pathogenesis of psoriatic arthritis. Best Practice & Research Clinical Rheumatology, 35(2), 101694.

Strand, V., & Kavanaugh, A. (2004). The role of interleukin-1 in bone resorption in rheumatoid arthritis. Rheumatology, 43(suppl_3), iii10-iii16.

Umezawa, Y. (2021). Psoriatic arthritis. The Journal of Dermatology, 48(6), 741-749.

Veale, D. J., & Fearon, U. (2018). The pathogenesis of psoriatic arthritis. The Lancet, 391(10136), 2273-2284.

Yokose, K., Sato, S., Asano, T., Yashiro, M., Kobayashi, H., Watanabe, H., . . . Yatsuhashi, H. (2018). TNF-α potentiates uric acid-induced interleukin-1 β (IL-1 β) secretion in human neutrophils. Modern Rheumatology, 28(3), 513-517.

Zabotti, A., De Lucia, O., Sakellariou, G., Batticciotto, A., Cincinelli, G., Giovannini, I., . . . Aletaha, D. (2021). Predictors, risk factors, and incidence rates of psoriatic arthritis development in psoriasis patients: a systematic literature review and meta-analysis. Rheumatology and Therapy, 8(4), 1519-1534.