Cytogenetic study of dental diagnostic x-ray workers

https://doi.org/10.53730/ijhs.v6nS6.9165

Authors

  • Asrar Kamil Salman Department of biology/ College of education for pure science/ University of Wasit
  • Sada Jasim Abdulameer Department of biology/ College of education for pure science/ University of Wasit

Keywords:

X-rays, chromosomal changes, ionizing radiation, X-ray technicians

Abstract

Background and Objectives: studying chromosome changes for dental x-ray workers in the city of kut and diagnosing those abnormalities resulting from continuous exposure to radiation. Exposure and Methods: The chromosomes of those exposed to dental X-rays were analyzed and studied using cytogenetic analysis to detect chromosomal aberrations caused by ionizing radiation after collecting blood samples from those exposed to Al-Zahraa and Al-Karama Teaching Hospital as well as from the Taiba Specialized Center in addition to the dental clinics in Wasit. Results: Chromosomal analysis of all subjects showed chromosomal aberrations, and most of the aberrations were structural changes that were divided according to the ratio of their frequencies. The largest percentage was for the gap chromosome type (44%), chromosomal-aberration dicentric (16%) and chromosomal-aberration gab and dicentric (8%). Conclusion: Dental diagnostic x-rays have an effect on the chromosomes of workers exposed to a long-term cumulative exposure period, as it leads to the appearance of aberrations of the type chromosomal aberration (gab chromatid ) is the most frequent in this stuy.

Downloads

Download data is not yet available.

References

Abolfazl Movafagh, Maleki F, Fadaie S, Azargashb E 2007. Persistent unstable chromosomal aberrations in lymphocytes of radiotherapy workers after 1st mitotic division in Tehran, Iran. Pak J Med Sci, 23(2) : 254- 258.

Alihossein Siberia,b, Elahe Salari c, Sayyed Mahmood Latifid 2013 Elsevier B.V. Cytogenetic analysis in lymphocytes from radiation workers exposed to low level of ionizing radiation in radiotherapy, CT-scan and angiocardiography units.

Amal Jabbar Matar, Abdul Sahib Kazem Ali, Ali Hassan Faraj et al. ,,2015.Biological genetic standardisation of radiation workers, Baghdad, Iraq. E-mail: ameljm20Q6@yahoo.com.

Ameerunnisa, Chaya M. David, Bangalore, Karnataka, India, Kamla-Raj 2011.Exposed to Diagnostic X-rays.

Balasem, A.N. and Ali, A.K. ;Mosa, H.S. &Hussain, K.O. (1992). Chromosome aberration analysis in peripheral lymphocytes of radiation workers. Mut. Res. 271 : 209-211.

Benavente-Garcı´a O, Castillo J, Lorente J, Alcaraz M, Ya´nez J, Martı´nez C, et al. Antiproliferative activity of several phenolic compounds against melanoma cell lines. Agroo Food Industries Hi-tech 2005; 16: 30–34.

Boffetta, P.; van der Hel, O.; Norppa, H.; Fabianova, E.; Fucic, A.; Gundy, S.; Lazutka, J.; Cebulska-Wasilewska, A.; Puskailerova, D.; Znaor, A.; et al. Chromosomal Aberrations and Cancer Risk: Results of a Cohort Study from Central Europe. Am. J. Epidemiol. 2007, 165, 36–43.

Bonassi, S.; Norppa, H.; Ceppi, M.; Strömberg, U.; Vermeulen, R.; Znaor, A.; Cebulska-Wasilewska, A.; Fabianova, E.; Fucic, A.;

Gundy, S.; et al. Chromosomal Aberration Frequency in Lymphocytes Predicts the Risk of Cancer: Results from a Pooled Cohort Study of 22 358 Subjects in 11 Countries. Carcinogenesis 2008, 29, 1178–1183.

Cintia, K. and Ilc, M. (2002). Cytogenetic biomonitoring of Brazillian dentists occupationally exposed to low doses of X radiation. Pesqui Odontol Bras, 16(3): 196-201.

De Pascalis, I.; Pilato, B.; Mazzotta, A.; Dell’Endice, T.S.; Rubini, V.; Simone, G.; Paradiso, A.; Aiello, V.; Mangia, A. Sister Chromatid Exchange: A Possible Approach to Characterize Familial Breast Cancer Patients. Oncol. Rep. 2015, 33, 930–934.

Feinendegen LE. Evidence for beneficial low level radiation effects and radiation hormesis. Br J Radiol 2005;78:3–7.

Geleick D, Mu ̈ller H, Matter A, Torhorst J, Regenass U: (1990). Cytogeneticsof breast cancer. Cancer Genet Cytogenet, 46:217–22.

Hall, J.E. (2000) ‘Doses and risks in diagnostic radiology, interventional radiology and cardiology, and nuclear medicine’, in J.E. Hall (Ed.) Radiobiology for the Radiologist, 5th ed., Philadelphia: Lippincott Williams and Wilkins, pp.199–233.

Hall, J.; Jeggo, P.A.;West, C.; Gomolka, M.; Quintens, R.; Badie, C.; Laurent, O.; Aerts, A.; Anastasov, N.; Azimzadeh, O.; et al. Ionizing Radiation Biomarkers in Epidemiological Studies—An Update. Mutat. Res. 2017, 771, 59–84.

Hall, E. J. & Giaccia, A. J., editors. Radiobiology for the radiologist. 7th ed. Philadelphia: Wolters Kluwer Health; p. 25–34 (2011).

Jain, A. K., Singh, D., Dubey, K., Maurya, R. and Pandey, A. K. (2017) ‘Chromosomal Aberrations’, in Kumar, A., Dobrovolsky, V., Dhawan, A., and Shanker, R. (eds) Mutagenicity:Assays and Applications. 1st edn. Ahemdabad, India: Academic Press, pp. 69–83.

Lee, C.; Yong,A.; Michele ,M.; Doody, D.; Preston, M.; Kampa, M.; Ramsey, E. and Ward M.(2010) Diagnostic X-ray examinations and

Lobrich, M. & Jeggo, P. A Process of Resection-Dependent Nonhomologous End Joining Involving the Goddess Artemis. Trends /j.tibs.2017.06.011 (2017).

Marcon, F., Andreoli, C., Rossi, S., Verdina, A., Galati, R. and Crebelli, R. (2003) ‘Assessment of individual sensitivity to ionizing radiation and DNA repair efficiency in a healthy population’, Mutat. Res., Vol. 541, pp.1–8.

M. Ballardin, A. Antonelli, M. Cipollini, P. Fallahi, R. Scarpato, A. Tomei, C. Traino, R. Barale, Induction of chromatid-type aberrations in peripheral blood lymphocytes of hospital workers exposed to very low doses of radiation, Mutat. Res. 626 (2007) 61–68.

Natarajan, A.T.; Boei, J.J.W.A. Formation of Chromosome Aberrations: Insights from FISH. Mutat. Res./Rev. Mutat. Res. 2003, 544, 299–304.

Orrenius S, Nicotera P and Zhivotovsky B: Cell death mechanisms and their implications in toxicology.Toxicol Sci 119(1): 3-19, 2011.

P. K. Gadhia, Nehal Shah, Sweta Nahata, Swati Patel, Krupa Patel, Meonis Pithawala and Dipesh Tamakuwala 2004. Cytogenetic Analysis of Radiotherapeutic and Diagnostic Workers Occupationally Exposed to Radiations.

Kazha M. Sulaiman 2017. Chromosomal aberrations in the peripheral blood lymphocytes of Dental Radiographers who were exposed to diagnostic X-ray in Erbil city / Iraqi Kurdistan region.

Rozgaj R, Kasuba V, Sentija K, Prlic I 1999. Radiation induced chromosomal aberration and hematological alterations in hospital workers, Occup Med, 49: 353-360.

Sevankaev, A.V. ( 2000) .Results of cytogenetic studies of the consequences of theChernobyl accident, Radiats. Biol. Radioecol. 40(2000) 589-595.

Stuart, C. ( 1967) Effects of Low-Level Radiation on Chromosomes. J Dent Res, 46: 1177-1181. list7. Anonymous news. Journal of nuclear medicine, 2003, necessarily contain a larger proportion of stem cells and developing cells that are likely to travel through the G2 stages of the cell cycle (Gonzalez et al.,2007).

Tafreshi, N.K.; Doligalski, M.L.; Tichacek, C.J.; Pandya, D.N.; Budzevich, M.M.; El-Haddad, G.; Khushalani, N.I.; Moros, E.G.; McLaughlin, M.L.; Wadas, T.J.; et al. Development of Targeted Alpha Particle Therapy for Solid Tumors. Molecules 2019, 24, 4314.

Tommasino, F.; Durante, M. Proton Radiobiology. Cancers 2015, 7, 353–381. Wolff, S. (1982). Sister chromatid exchange. John Wiley and Sons. New York ,Chichester, Toronto.

Tubbs, A. and Nussenzweig, A. (2017) ‘Endogenous DNA Damage as a Source of Genomic Instability in Cancer’, Cell, 168(4), pp. 644–656. doi: 10.1016/j.cell.2017.01.002.

Uma1, A.; Dhananjay, S.; Kotasthane, and Tirou ,T. (2014) Chromosomal aberrations: A tool for early diagnosis of cancer in smokers in a rural Pondicherry population, India Int.J.Curr.Microbiol.App.Sci 3(10) 587-593587.

Unscear. Sources and Effects of Ionizing Radiation, Report to the General Assembly with Scientific Annexes; UNSCEAR: New York, NY, USA, 2008.

Verdorfer ,; Neubauer ; Letzel s. ; Angerer. ; Arutyunyan R. ; Martus p. ; Wucherer M. and Gebhart E.( 2001).Chromosome painting for cytogenetic monitoring of occupationally exposed and non-exposed groups of human individuals. Mutat Res, 491(1-2): 97 -109.

Wouters, B. G. Cell death after irradiation: how, when and why cells die. In: Joiner, M. C. & van der Kogel, A. editors. Basic clinical radiobiology. 4th ed. Boca Raton: CRC Press; p. 27–40 (2009).

Yassen N.Y. (1990). Cytogenetic study on human colorectal cancer cells. PhD. Thesis submitted University of Sheffield Medical School.

Published

17-06-2022

How to Cite

Salman, A. K., & Abdulameer, S. J. (2022). Cytogenetic study of dental diagnostic x-ray workers. International Journal of Health Sciences, 6(S6), 32–43. https://doi.org/10.53730/ijhs.v6nS6.9165

Issue

Section

Peer Review Articles

Most read articles by the same author(s)