Anti-Gout arthritic activity of ethanolic and methanolic seed extracts of Pedalium Murex: An in vitro and in silico studies

Lakshmi P.S Deepa; Rajeswary Hari; A Sandhiya; Rajan. K Pruthiv

doi:10.53730/ijhs.v6nS5.9366

Authors

Deepa Lakshmi P.S Department of Biotechnology, Dr. MGR Educational and Research Institute, Deemed to be University, Maduravoyal, Chennai, India
Rajeswary Hari
rajihar@gmail.com
Department of Biotechnology, Dr. MGR Educational and Research Institute, Deemed to be University, Maduravoyal, Chennai, India
Sandhiya. A Department of Biotechnology, Dr. MGR Educational and Research Institute, Deemed to be University, Maduravoyal, Chennai, India
Pruthiv Rajan. K Department of Biotechnology, Dr. MGR Educational and Research Institute, Deemed to be University, Maduravoyal, Chennai, India

Keywords:

Pedalium murex, anti - gout arthritic, MSU, xanthine oxidase

Abstract

The present investigation is undertaken to evaluate the comparative anti gout activity of the ethanolic (EEPM) and methanolic (MEPM) seed extracts of Pedalium murex. The Invitro anti gout arthritic activity of the plant extracts was evaluated using xanthine oxidase and protease enzyme inhibition as well as membrane stabilization using well established protocols. In silico anti arthritic activity was studied using molecular docking analysis from 10 GCMS derived compounds with that of the immunuo stimulatory proteins TLR2, TLR4, MMP2 and MMP7 to find out the suitable antagonistic ligand for these proteins. In the present investigation the EEPM extract showed better anti gout activity comparable to the MEPM extract in terms of xanthine oxidase and protease enzyme inhibition as well as membrane stabilization. The anti out arthritic activity of EEPM was comparable to the positive control used in the present study. In the In silico studies among the 10 compounds docked for the proteins TLR2, TLR4, MMP2 and MMP7, three compounds namely 11 -Eicosenoic Acid Methyl ester, Docosanoic Acid, Methyl ester and 1,2Benzendicarboxylic Acid, mono (2-ethylhexyl) ester could inhibit the above mentioned immuno stimulatory proteins and there by suppress the reactions observed in the gouty arthritis.

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References

WalizaAnsar and ShyamasreeGhosh.(2016) Inflammation and Inflammatory Diseases,

Markers, and Mediators: Role of CRP in Some Inflammatory Diseases Biology of C Reactive Protein in Health and Disease. Mar 24: 67–107

Meyer et al.,2005 .Response to traditional disease-modifying anti-rheumatic drugs in indigent South Africans with early rheumatoid arthritis613–619 (2012)

Koeberle, A.; andWerz, O.(2014). Multi-target approach for natural products in inflammation. Drug Discov Today. 2014

Dinesh kumar Patel et al., 2011Pedalium murex Linn.: An overview of its phytopharmacological aspects. Asian journal of tropical medicine.Volume 4, Issue 9, September 2011, S1995-7645(11)60186-7

Dey D, Das MN (1995) Pharmacognostical studies of root and fruit of Gokshura (Pedalium murex Linn.). BMEBR 16(1–2):54–65

Kim KY, Ralph Schumacher H, Hunsche E, Wertheimer AI, Kong SX.

A literature review of the epidemiology and treatment of acute gout. Clin Ther 2003;25(6):1593-617.

Bhakuni et al., 1992Scrutinizing the aqueous extract of leaves of pedalium murex for the antiulcer activity in rats.Pakistan Journal of Pharmaceutical Sciences . Jun2010, Vol. 23, p295-299.

Sadique et.al.,Sadique J, Al-Rqobah WA, Bugharlth ME, El-Gindy AR (1989). The bioactivity of certain medicinal plants on the stabilization of RBC membrane system. Fitoterapia. Vol. LX No.6 pp. 525-532

Martillo, Miguel A.; Nazzal, Lama; Crittenden, Daria B. (2014). The Crystallization of Monosodium Urate. Current Rheumatology Reports, 16(2), 400–126. doi:10.1007/s11926-013-0400-9

Rakhmonov, O. M., Shadmanov, A. K., & Juraev, F. M. (2021). Results of endoscopic treatment of benign prostatic hyperplasia in patients with metabolic syndrome. International Journal of Health & Medical Sciences, 5(1), 21-25. https://doi.org/10.21744/ijhms.v5n1.1811

Liu, Yanzhuo; Duan, Chenfan; Chen, Honglei; Wang, Chenlong; Liu, Xiaoxiao; Qiu, Miao; Tang, Honglin; Zhang, Feng; Zhou, Xiaoyang; Yang, Jing (2018). Inhibition of COX-2/mPGES-1 and 5-LOX in macrophages by leonurine ameliorates monosodium urate crystal-induced inflammation. Toxicology and Applied Pharmacology, 351(), 1–11. doi:10.1016/j.taap.2018.05.010

Albrecht E, Waldenberger M, Krumsiek J, Evans AM, Jeratsch U, Breier M, et al. Metabolite profiling reveals new insights into the regulation of serum urate in humans. Metabolomics, 2014; 10:141–151

Liu-Bryan R, Pritzker K, Firestein GS, Terkeltaub R:2005. TLR2 signaling in chondrocytes drives calcium pyrophosphate dehydrate and monosodium urate crystal-induced nitric oxide generation. J Immunol 174:5016-5023.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Liu-Bryan R, Scott P, Sydlaske A, Rose DM, Terkeltaub R: 2005. Innate immunity conferred by toll-like receptors 2 and 4 and myeloid differentiation factor 88 expression is pivotal to monosodium urate monohydrate crystal-induced inflammation. ArthritisRheum 52:2936-2946

Haskard, D. O., and R. C. Landis. 2002. Interactions between leukocytes and endothelial cells in gout: lessons from a self-limiting inflammatory response.Arthritis Res. 3:91.

Landis, R. C., and D. O. Haskard. 2001. Pathogenesis of crystal-induced inflammation.Curr. Rheumatol. Rep. 3:36