Citrullus colocynthis loaded zinc nanoparticle (C.cZnO): A potential gout healer in modulating antioxidant status and lysosoaml enzymes in MSU crystal Induced PBMC cells
Keywords:
antioxidant enzymes, Citrullus Colocynthis, zinc oxide nanoparticlesAbstract
The aim of the present investigation is to study the effect of C.colocynthis loaded zinc nanoparticle (C.cZnO) on the antioxidant status and lysozomal enzymes in PBMC cells induced with monosodium urate crystals. The Zinc nano particles of C.colocynthis was prepared. Approximately 1×106 cells/well seeded human PBMC were divided in to five groups and treated in the following manner. Group I served as control, Group II cells were treated with 1mg/ml MSU crystals, Group III and Group IV cells were induced with MSU crystals and received 250µg/ml and 500 µg/ml of C. colosynthis zinc nanoparticle (C.cZno) respectively and were incubated at 37°C for 24 hrs in the CO2 incubator. Group-IV cells were treated with colchicine (1µg/ml) the positive control used in the present study. The enzymatic and non enzymatic antioxidants as well as lysosomal enzymes were analysed using the established procedures. The extent of lipid peroxidation was also measured.
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