Molecular detection of a polymorphism in the p53 codon 72 gene and its association with prostate cancer
Keywords:
molecular detection, polymorphism, gene, prostate cancerAbstract
The role of the current study in detecting polymorphisms in the gene P53codon72, a gene that has morphological polymorphism and has an important role in the incidence of diseases, especially cancers such as prostate cancer, samples for the study were collected from 50 patients with prostate cancer from the auditors of the Imam Hussein Center for Cancer and Diseases Blood in Al-Hussein, peace be upon him, Teaching Hospital - Holy Karbala Governorate - Iraq After diagnosis by a specialist surgeon and 50 phenotypically healthy individuals as a control group, the (DNA) was extracted from the blood of both healthy and all patients study groups, and molecular detection of polymorphism of nucleotides was performed in Genes of the study using PCR-RFLP Molecular detection results in P53codon72 The results showed that there were highly significant differences between the genotype (Pro-Pro) and between the infected and healthy groups as well as the genotype (Arg-Arg), while there was no significant relationship between the genotype (Arg-Pro) and between the infected and healthy groups. The results also showed highly significant differences between the three genotypes (Arg/ Arg, Aro/Pro, Pro/Pro) and between the two groups sick and healthy.
Downloads
References
Benabdelkrim, M., Djeffal, O. and Berredjem, H. (2018) ‘GSTM1 and GSTT1 polymorphisms and susceptibility to prostate cancer: A case-control study of the Algerian Population’, Asian Pacific Journal of Cancer Prevention, 19(10), pp. 2853–2858. doi: 10.22034/APJCP.2018.19.10.2853.
Boo, Y. C. (2021) ‘Arbutin as a skin depigmenting agent with antimelanogenic and antioxidant properties’, Antioxidants, 10(7), pp. 1–22. doi: 10.3390/antiox10071129.
Cannarella, R. et al. (2021) ‘Endocrinology of the Aging Prostate: Current Concepts’, Frontiers in Endocrinology, 12(February), pp. 1–13. doi: 10.3389/fendo.2021.554078.
Donehower, L. A. et al. (2019) ‘Integrated Analysis of TP53 Gene and Pathway Alterations in The Cancer Genome Atlas’, Cell Reports, 28(5), pp. 1370-1384.e5. doi: 10.1016/j.celrep.2019.07.001.
Doosti, A. and Dehkordi, P. G. (2011) ‘The p53 codon 72 polymorphism and association to prostate cancer in iranian patients’, African Journal of Biotechnology, 10(60), pp. 12821–12825. doi: 10.5897/ajb11.1442.
Gems, D. (2021) ‘The hyperfunction theory: an emerging paradigm for the biology of aging’, Preprints, (August), pp. 1–39. doi: 10.20944/preprints202108.0552.v1.
Hong, Y. et al. (2019) ‘Association between tumor necrosis factor alpha gene polymorphisms and multiple myeloma risk: an updated meta-analysis’, Hematology (United Kingdom), 24(1), pp. 216–224. doi: 10.1080/16078454.2018.1552341.
Huang, R. and Zhou, P. K. (2021) DNA damage repair: historical perspectives, mechanistic pathways and clinical translation for targeted cancer therapy, Signal Transduction and Targeted Therapy. Springer US. doi: 10.1038/s41392-021-00648-7.
Huang, S. P. et al. (2004) ‘P53 Codon 72 and P21 Codon 31 Polymorphisms in Prostate Cancer’, Cancer Epidemiology Biomarkers and Prevention, 13(12), pp. 2217–2224.
Jawad, Z. N. et al. (2018) ‘ASSOCIATION OF MDM2 ( T309G ) GENE POLYMORPHISM WITH INTERSTITIAL URINARY BLADDER CANCER’, 18(2), p. 5075.
Jawad, Z. N. and Awad, W. (2020) ‘Association of urokinase and Vitamin D receptor genes SNPs and urolithiasis in an Iraqi population’, Meta Gene, 24(February), p. 100679. doi: 10.1016/j.mgene.2020.100679.
Jawad, Z. N., Kamal, A. R. and Awad, W. (2020) ‘The effect of montelukast on CD14 gene expression in asthmatic patient in Kerbala’, AIP Conference Proceedings, 2290(December), pp. 10–13. doi: 10.1063/5.0029590.
Jawad, Z. N., Mohammed, Z. and Jeddoaa, A. (2019) ‘Molecular Detection of MTHFR gene Polymorphisms in Transitional Urinary Bladder Cancer Patients’, 11(2), p. 2019.
Jawad, Z. N., Rasool, K. A. and Awad, W. (2020) ‘The relationship between smoking and urokinase gene 3’-UTR T/C expression on occurrence of bladder cancer’, Indian Journal of Forensic Medicine and Toxicology, 14(1), pp. 1169–1171. doi: 10.37506/v14/i1/2020/ijfmt/193066.
Kaya, Z. et al. (2021) ‘Evaluation of TP53 codon 72 polymorphism in esophageal cancer susceptibility in Eastern Anatolia region of Turkey’, Eastern Journal of Medicine, 26(3), pp. 388–395. doi: 10.5505/ejm.2021.65707.
Lopez, P. et al. (2021) ‘Associations of fat and muscle mass with overall survival in men with prostate cancer: a systematic review with meta-analysis’, Prostate Cancer and Prostatic Diseases, (May), pp. 1–12. doi: 10.1038/s41391-021-00442-0.
Louis, D. N. et al. (2021) ‘The 2021 WHO classification of tumors of the central nervous system: A summary’, Neuro-Oncology, 23(8), pp. 1231–1251. doi: 10.1093/neuonc/noab106.
Mittal, R. D. et al. (2011) ‘Role of Functional Polymorphisms of P53 and P73 Genes with the Risk of Prostate Cancer in a Case-Control Study from Northern India’, Archives of Medical Research, 42(2), pp. 122–127. doi: 10.1016/j.arcmed.2011.03.001.
Mostaid, M. S. et al. (2021) ‘Elevated serum expression of p53 and association of TP53 codon 72 polymorphisms with risk of cervical cancer in Bangladeshi women’, Plos One, 16(12), p. e0261984. doi: 10.1371/journal.pone.0261984.
Van Poppel, H. et al. (2021) ‘Prostate-specific Antigen Testing as Part of a Risk-Adapted Early Detection Strategy for Prostate Cancer: European Association of Urology Position and Recommendations for 2021’, European Urology, (2021), pp. 1–9. doi: 10.1016/j.eururo.2021.07.024.
Rogler, A. et al. (2011) ‘P53 codon 72 (Arg72Pro) polymorphism and prostate cancer risk: Association between disease onset and proline genotype’, Pathobiology, 78(4), pp. 193–200. doi: 10.1159/000326767.
Skandalaki, A., Sarantis, P. and Theocharis, S. (2021) ‘Pregnane x receptor (Pxr) polymorphisms and cancer treatment’, Biomolecules, 11(8). doi: 10.3390/biom11081142.
Taha, M. Z. and Ali, H. H. (2020) ‘Relationship between Vitamin D and some clinical variables for benign prostatic hyperplasia in Iraqi patients’, 7334(December), pp. 7331–7334.
Tiwari, R. and Fleshner, N. (2021) ‘The role of metformin, statins and diet in men on active surveillance for prostate cancer’, World Journal of Urology, (October). doi: 10.1007/s00345-021-03858-4.
Thaib, P. K. P., & Rahaju, A. S. (2022). Clinicopathological profile of clear cell renal cell carcinoma. International Journal of Health & Medical Sciences, 5(1), 91-100. https://doi.org/10.21744/ijhms.v5n1.1846
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
