Study the beneficial role of laser irradiation combination with indirect swim-up sperm preparation technique against oxidative DNA damage in infertile men

Noor M. Kamel; Hussain Kh. Kadhem; Bushra R. AL-Azzawi; Hayder A. L. Mossa

doi:10.53730/ijhs.v6nS5.9644

Authors

Noor M. Kamel High Institute for Infertility Diagnosis and Assisted Reproductive Technologies Al- Nahrain University, Baghdad –Iraq
Hussain Kh. Kadhem PhD. infertility and Clinical Reproduction, Al-Batool Teaching hospital-Diyala, Ministry of Health, Iraq
Bushra R. AL-Azzawi PhD. infertility and Clinical Reproduction, Al- Farah Specialist Fertility Center, Baghdad, Iraq
Hayder A. L. Mossa
haydermossa@googlemail.com
Assistant Professor Dr. High Institute for Infertility Diagnosis and Assisted Reproductive Technologies Al- Nahrain University, Baghdad – Iraq

Keywords:

Light Amplification by Stimulated Emission of Radiation (LASER), Indirect Swim Up(ISU), 8-Hydroxydeoxyguanosine (8-OHDG)

Abstract

Background: In order to offer successful assisted reproductive procedures, a variety of in vitro sperm preparation techniques were created to separate normal and motile spermatozoa from other constituents of the sample. Much research on the laser as a sperm motility stimulant has been undertaken, and the results have indicated that the Laser has a good effect on sperm activation in vitro and improves progressive forward movement. Objective: This study is aimed to identify the differences in sperm activation by ISU with or without laser methods and compare them. And detection of oxidative damage to the DNA before and after laser by assessment of the 8-Hydroxydeoxyguanosine (8-OHDG) as a biomarker. Patients and Methods: The current study was conducted on 30 semen samples, divided into two groups (Asthenozoospermia and Normozoospermia individuals), during the period of attendance at the infertility clinic at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University. From November 2021 until March 2022. Each sperm sample was separated into three portions.

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