The effects of gene polymorphisms in tumor necrosis factor-α on the susceptibility of type I diabetes mellitus in an Iraqi population

Hadeel Mohsen Abbas; Rand Muhammed Abdul-Hussein Al-Husseini

doi:10.53730/ijhs.v6nS8.9750

Authors

Hadeel Mohsen Abbas
hadeelmohsen514@gmail.com
Faculty of Education for Girls, University of Kufa, Iraq
Rand Muhammed Abdul-Hussein Al-Husseini Professor, Faculty of Science, University of Kufa, Iraq

Keywords:

T1DM, (TNF-α) gene -308G/A, polymorphism, RFLP- PCR

Abstract

Background: Type 1 diabetes mellitus (T1DM) is a chronic, immune-mediated disease characterized by the destruction of insulin-producing β cells in the pancreas. This study investigated the influence of single nucleotide polymorphisms (SNP) in tumor necrosis factor (TNF-α) –308 G/A (rs1800629,) on T1DM patients. Method: The study was included 75 T1DM patients and 25 healthy subjects. The SNP–308 G/A TNF-α gene was detected by using polymerase chain reaction restriction fragment length polymorphism (RFLP-PCR) technique in T1DM patients and controls. Results: The genotype distribution results of the -308G/A SNP of Tumor Necrosis Factor - α (TNF- α)-308 G/A gene showed significant difference (p<0.05) between controls (GG: n= 24, 96%; GA: n=1, 4%; AA: n=0, 0%) and T1DM patients (GG: n= 44, 58.67%; GA: n=21, 28%; AA: n=10, 13.33%).These results showed an increased in GA, AA genotype and G allele of the TNF- α(-308) G/A in T1DM patients than controls, and they were significantly more likely than controls to have the mutant allele (OR=18.431, 95%CI= 2.464-137.855, p=0.00001). These results indicate a possible role for the G genotype in T1DM disease.

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