Analysis of Proteus mirabilis proteins by using SDS-page technique in rheumatoid arthritis patients
Comparative study
Keywords:
SDS-page, rheumatoid arthritis, RA, Proteus mirabilis, proteinsAbstract
This study was aimed to detecting difference in proteins separation by SDS-Page for Proteus mirabilis isolated from urine of non-Rheumatoid arthritis patients have urinary tract infection , urine from Rheumatoid arthritis patients have urinary tract infection. Two hundred (200) urine sample collected from non RA patient30 urine sample from RA patient . These clinical samples were collected and cultured on blood agar and macConky agar. 32 isolates were identified as p. mirabilis by using biochemical test and VITEK 2 system used to complete the identification. The current study used SDS-Page technique for proteins analysis of Proteus mirabilis for both RA patient and non RA patient to detect the differences between them and the result appeared that there are no differences in total protein, insoluble proteins and soluble protein at different pH. Values (6.0, 7.0 & 8.0) at 37 cº, also the result shown differences in the cell –free culture supernatants between Proteus mirabilis from urine of both rheumatoid arthritis patients and non-rheumatoid arthritis patient in pH.8.0 at 37 cº in 7 h. by band appeared in pH.8.0 at 37 cº in 7 h in RA .and there is no band in non RA at the same condition.
Downloads
References
Al-Duliami, A. A., Nauman, N. G., Hasan, A. R. S., and Al-Azawi, Z. H., 2011. Virulence factors of Proteus Mirabilis isolated from patients otitis media in Baquba and it’s peripheries. Diyala Journal of Medicine, 1(1), pp.69-75.
Ebringer, A. and Rashid, T., 2006. Rheumatoid arthritis is an autoimmune disease triggered by Proteus urinary tract infection. Clinical and Developmental Immunology, 13(1), pp.41-48.
Ebringer, A., Rashid, T. and Wilson, C., 2010. Rheumatoid arthritis, Proteus, anti-CCP antibodies and Karl Popper. Autoimmunity Reviews, 9(4), pp.216-223.
Gibofisky, A., 2014. Epidemiology, pathology and diagnosis of rheumatoid arthritis :Asynopsis. The American journal of managed care, 20(7 suppl ), pp.S128-35.
Goronzy, J. and Weyand, C., 2009. Developments in the scientific understanding of rheumatoid arthritis. Arthritis Research & Therapy, 11(5), p.249.
Hasan,T.H.; Al-Harmoosh, R. A. and Al-khilkhali, H. J. B., 2020. Identification of HIV virus in Najaf city, Iraq. International Journal of Research in Pharmaceutical Sciences, 11(3), pp.4866-4871.
Hayder, T. and Aljanaby, A.A.J., 2019. Genotypic Characterization of Antimicrobial Resistance-Associated Genes in Citrobacter Freundii Isolated from Patients with Urinary Tract Infection in Al-Najaf Governorate-Iraq. OnLine Journal of Biological Sciences, 19(2), pp.132-145.
Kadhum, H.A. and Hasan, T.H., 2019. The study of bacillus subtils antimicrobial activity on some of the pathological isolates. International Journal of Drug Delivery Technology, 9(2), pp.193-196.
Laemmli, U. K., 1970. SDS-page Laemmli method. Nature, 227, pp. 680-685.
Loomes, L.M., Senior, B.W. and Kerr, M.A., 1992. Proteinases of Proteus spp.: purification, properties, and detection in urine of infected patients. Infection and immunity, 60(6), pp.2267-2273.
Lorenz, W., Buhrmann, C., Mobasheri, A., Lueders, C. and Shakibaei, M., 2013. Bacterial lipopolysaccharides form procollagen-endotoxin complexes that trigger cartilage inflammation and degeneration: implications for the development of rheumatoid arthritis. Arthritis Research & Therapy, 15(5), p.R111.
Nicolle, L.E., 2014. Antimicrobial stewardship in long term care facilities: what is effective?. Antimicrobial resistance and infection control, 3(1), pp.1-7.
Piccini, C.D., Barbe, F.M. and Legnani-Fajardo, C.L., 1998. Identification of iron-regulated outer membrane proteins in uropathogenic Proteus mirabilis and its relationship with heme uptake. FEMS microbiology letters, 166(2), pp.243-248.
Pretorius, E., Akeredolu, O., Soma, P. and Kell, D., 2017. Major involvement of bacterial components in rheumatoid arthritis and its accompanying oxidative stress, systemic inflammation and hypercoagulability. Experimental Biology and Medicine, 242(4), pp.355-373.
Rashid, T. and Ebringer, A., 2007. Rheumatoid arthritis is linked to Proteus—the evidence. Clinical Rheumatology, 26(7), pp.1036-1043.
Schaffer, J.N. and Pearson, M.M., 2017. Proteus mirabilis and urinary tract infections. Urinary Tract Infections: Molecular Pathogenesis and Clinical Management, pp.383-433.
Widana, I.K., Sumetri, N.W., Sutapa, I.K., Suryasa, W. (2021). Anthropometric measures for better cardiovascular and musculoskeletal health. Computer Applications in Engineering Education, 29(3), 550–561. https://doi.org/10.1002/cae.22202
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
