Investigation of clostridium perfringens in contaminated lobbies and surgical theater and showing their resistant to metronidazole

Sahar Kasim Abd  Al-Ameer; Maytham Abas Makki

doi:10.53730/ijhs.v6nS5.9929

Authors

Sahar Kasim Abd Al-Ameer College of Science, Al-Muthanna University, Al-Muthanna/Iraq
Maytham Abas Makki College of Science, Al-Muthanna University, Al-Muthanna/Iraq

Keywords:

Chromogenic agar, Clostridium perfringens, Contamination percentage, Antibiotic resistant

Abstract

Objective: The study aimed to isolation and diagnosis Clostridium perfringens from Al-Rammathia general hospital environment (lobbies and operating theaters) and determining the percentages of Clostrdium perfringens contamination in lobbies and operating theaters and comparing them with each other. Methodology: Collected in the current study (188) sample of the hospital environment, which included walls and flooring tools and beds for lobbies and operations room, in Al-Rumaitha General hospital and for a period ranging from October (2021) until April(2022(these samples were taken by sterile cotton swabs and used to transport them to the microbiology laboratory at the College of Science / University of Muthanna in media of Clostridium perfringens broth to perpetuate them, then the medium containing the sample was boiled in a water bath at 100 degrees Celsius for 20 minutes and incubated anaerobic using Anaerobic jar and gas-generating bags (Gas pak). Which provides the anaerobic conditions necessary for bacterial growth through oxygen reduction inside the container’s atmosphere at a temperature of 37 for a period ranging from 24-48 hours. It was then re-implanted on the final media for purification before being used in diagnostic testing.

Downloads

Download data is not yet available.

References

Gravel D, Taylor G, Ofner M, Johnston L, Loeb M, Roth VR, et al. A point prevalence survey for healthcare-associated infections within Canadian adult acute-care hospitals. J Hosp Infect. 2007;66:243–8

Dancer SJ. Importance of the environment in meticillin-resistant Staphylococcus aureus acquisition: the case for hospital cleaning. Lancet Infect Dis. 2008;8:101–13.

Lugauska A. and Krikstaponis A. (2004) Filamentous fungi isolated in hospitals and some medical institutions in Lithuania .Indoor Built Environment. 13:101-113

Jain A, Venkatesh V, Agarwal J. Microbiology Practical Manual E-Book: 1st ed. Elsevier .India. 2018

Grass JE, Gould LH, Mahon BE. Epidemiology of foodborne disease outbreaks caused by Clostridium perfringens, United States, 1998–2010. Foodborne pathogens and disease. 2013 Feb 1;10(2):131-136.

Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clinical microbiology reviews. 2001 Apr 1;14(2):244-269.

Revitt-Mills SA, Rood JI, Adams V. Clostridium perfringens extracellular toxins and enzymes: 20 and counting. Microbiology Australia. 2015 Sep 17;36(3):114-117.

McDonel J.L. Toxins of Clostridium perfringens types A, B, C, D, and E.In Dorner F, Drews H (ed), Pharmacology of bacterial toxins. Pergamon Press, Oxford, England. 1986;477–517.

Petit L, Gibert M, Popoff MR. Clostridium perfringens: toxinotype and genotype. Trends in microbiology. 1999 Mar 1;7(3):104-110.

Huang, I. H., Raju, D., Paredes-Sabja, D., and Sarker, M.R. 2007. Clostridium perfringens: Sporulation, Spore Resistance and Germination. Bang. J. Microbiol. 24:1-8

Morello,: Mizer, H.E.; and Granat, P.A. (2006) Laboratory manual and Book in Microbiology Applications to Patient Care. 8th ed. McGraw- Hill, USA

MAcfaddin, J.E. (2000) individual biochemical test. In: Biochemical test for identification of medical bacteria (3rded) Macfaddin J. E. (ed.) p:27-439. Lionicott Williams & wilkins Co Blatimore. U.S.A

Tille PM. Bailey and Scott’s Diagnostic Microbiology-E-Book. 13th ed . Louis, Missouri : Elsevier. China. 2014.

Pommerville J.C. Alcamo’s Laboratory Fundamentals of Microbiology. 8th ed. Jones and Bartlett .USA. 2007.

Cappuccino JG, Welsh CT. Microbiology: A Laboratory Manual, Global edition. 11th ed. Pearson Education Limited. 2017.

Tilyakov, H. A., Valiyev, E. Y., Tilyakov, A. B., & Tilyakov, A. B. (2021). A new approach to surgical treatment of victims with pelvic and femoral fracture injuries, taking into account the severity of the condition and the severity of the injury. International Journal of Health & Medical Sciences, 4(3), 338-346. https://doi.org/10.31295/ijhms.v4n3.1763

CLSI (Clinical and Laboratory Standards Institute). Methods for antimicrobial susceptibility testing of bacteria. Setting the standard for quality in medical laboratory testing around the world. M100,28th ed. 2018.

Kelly CM, Heidir S, Cowan MK, Smith H. Microbiology, 5th ed. Aystems Approach, McGraw-Hill, USA. 2018

Tille PM. Baily and scott’s Diagnostic Microbiology- E- Book . 14th ed . St. Louis, Missouri : Elsevier. China. 2017.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Mahon CR, Lehman DC, Manuselis G. Textbook of Diagnostic Microbiology.5th ed. Saunders, Elsevier.Inc, China. 2015

Abdullatef, Fallah H., Bassim M. Al-khafaji, Anwar H. Jawad, and Qassim J. Talib. "The Bacterial Contamination of the Operating lounges in Hospitals of Al-Diwaniyah Province with the Two types of Intestinal E. coli and St. aureus Cutaneous." Al-Qadisiyah Journal Of Pure Science 26, no. 1 (2021): 1-9.

Siddiqui AR , Luby SP. (1998).High rate of discitis following surgery for prolapsed intervertebral discs at a hospital in Pakistan .Infection control and hospital epidemiology ,19(7):526-9.

Abdulsahib A., Salwan. ; Homood A., Bahija (2017). (The Bacterial Contamination of Operating Lobbies and Halls in Diwaniyah Hospitals). Al-Kufa university Journal for biology. Vol.9 , No.1 :347-360

Land KM, Johnson PJ. Molecular basis of metronidazole resistance in pathogenic bacteria and protozoa. Drug Resistance Updates. 1999 Oct 1;2(5):289-294