Molecular detection of some virulence gene in Acinetobacter spp. isolated from different drinking water samples in AL-Najaf Al-Ashraf Province
Keywords:
Acinetobacter spp., Ampc, MotA, biofilmAbstract
Contamination of drinking-water is a significant concern for public health throughout the world. Microbial hazards make the largest contribution to waterborne disease in developed and developing countries. Biofilm-producing bacteria were identified by the Congo Red Agar (CRA) method we obtained isolated of Acinetobacter spp. had ability to biofilm formation in medial , the antibiotic resistance screening test to a number of antibiotics on Acinetobacter spp. isolates. The results revealed that isolates of Acinetobacter spp showed a high resistance to amoxicillin, levofloxacin, cefoxitin and imipenem (100 %) followed by amoxicillin and clavulanic and cefoxitin in mediate resistance in Acinetobacter spp , while erthromycin did not show any resistance by Acinetobacter spp. the results indicated that the Ampc gene wasnot present in Acinetobacter baumanni and Acinetobacter lwoffii ,while the results indicated that the MotA gene was presented in Acinetobacter lwoffii as well as the MotB gene was presented in Acinetobacter baumanni and Acinetobacter lwoffii )
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Al-Hadraawy SK, Alhadrawi KK, Aljanaby IA, Aljanaby AA, Zabibah RS. Prevalence of pulmonary tuberculosis in Al-Najaf governate, Iraq. F1000Research. 2022 Jun 20;11(675):675.
Alhasnawi HMR, Ahmed Abduljabbar Jaloob Aljanaby AAJ. The immunological role of CD4 and CD8 in patients infected with Helicobacter pylori and stomach cancer. Gene Reports 26 (2022) 101500. https://doi.org/10.1016/j.genrep.2022.101500
Al-Hassan, A.R.(2012). A study of carbapenem resistance inAcinetobacter baumannii isolates from Kuwait.PhD thesis in University of Edinburgh.
Aljanaby AA, Al-Faham QM, Aljanaby IA, Hasan TH. Epidemiological study of Mycobacterium Tuberculosis in Baghdad Governorate, Iraq. Gene Reports. 2022 Mar 1;26:101467.
Almayali E. J. B. , Al-Muhanna A. S. and Al-Fatlawi B. A. (2017) . Molecular study to evaluate some virulence factors that associated with Bacterial Tonsillitis , staphylococcus aureus and pseudomonas aeruginosa . M.Sc. Thesis . Faculty of Science, University of Kufa .
Armbruster, C. E.; Mobley, H. L.; and Pearson, M. M. (2018). Pathogenesis of Proteus mirabilis infection. EcoSal Plus, 8(1).
Belas, R. (2014). Biofilms, flagella, and mechanosensing of surfaces by bacteria. Trends in microbiology, 22, 517-527.
Bergogne-Bérézin E, Towner JK.(1996). Acinetobacter spp. As nosocomialpathogens: microbiological, clinical,and epidemiological features. ClinMicrobiol Reviews, 9 (2):148-51.
Brooks, G.F.; Carroll, K.C.; Butel, J.S.; Morse, S.A. and Mietzner, T.A. (2013). Jawetz, Melnick and Adelberg’s Medical Microbiology. 26 th Edition, McGraw-Hill, New York.
Cerqueira , G.M. and Peleg , A.Y. (2011). Insights into Acinetobacter baumannii pathogenicity.IUBMB life,63:1055-1060.
Che, Y. S.; Nakamura, S.; Morimoto, Y. V.; Kami‐ike, N.; Namba, K.and Minamino, T. (2014). Load sensitive coupling of proton translocation and torque generation in the bacterial flagellar motor. Mole. microbio., 91, 175-184.
CLSI, CLSI M100-ED30: (2021) Performance Standards for Antimicrobial Susceptibility Testing, 31th Edition (2021).
Cornelis P (2008). Pseudomonas: Genomics and Molecular Biology (1st ed.). Caister Academic Press. ISBN 1-904455-19-0.
Cusick, K.; Lee, Y. Y.; Youchak, B. and Belas, R. (2012). Perturbation of FliL interferes with Proteus mirabilis swarmer cell gene expression and differentiation. J. of bacterio., 194, 437-447.
Dijkshoorn, L. ;Nemec, A. and Carretto, E. (2011). Acinetobacter baumannii, a troublesome guest in the hospital.GImPIOS. 1(2): 52-56.
Ghaima, K. K.; Hamid, H. H. and Hasan, S. F. (2017). Biofilm formation, antibiotic resistance and detection of mannose-resistant Proteus-like (MR/P) fimbriae genes in Proteus mirabilis isolated from UTI. International Journal of ChemTech Research, 10(5), 964-971.
Hadi, H. I. & Aljanaby, A. A. J. (2022). Helicobacter Pylori-oncogenic protein cytotoxin-associated gene A and assessment of CD14 and CD163 in duodenal ulcer and gastric cancer patients. International Journal of Health Sciences, 6(S2), 839–851. https://doi.org/10.53730/ijhs.v6nS2.5134
Kosmidis, C.; Poulakou, G.; Markogiannakis, A.; and Daikos, G. L. (2012). Treatment Options for Infections Caused by Carbapenem-resistant Gram-negative Bacteria. European Infect. Dis. 6(1): 28–34..Microbiol.,56: 191–200.
Kostakioti M. ; Hadjifrangiskou M. and Hultgren S. J. ; . Bacterial Biofilms: Development, Dispersal, and Therapeutic Strategies in the Dawn of the Postantibiotic Era. Journal List Cold Spring Harb Perspect Med v.3(4); 2013 Apr PMC3683961
Kurcik-Trajkovska B (2009) Acinetobacter spp. - A serious enemy threatening hospitals worldwide.Maced J Med Sci2: 157–162. Kutateladze, M., and Adamia, R. (2010). Bacteriophages as potential new therapeutics to replace or supplement antibiotics. Trends Biotechnol. 28, 591–595.
Lee, H. W.; Koh, Y. M.; Kim, J.; Lee, J. C.; Lee, Y. C.; Seol, S. Y. and Cho, D. T. (2008). Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces.Clin.Microbiol. Infect .14: 49-54.
MacFaddin, J.F. (2000). Biochemical Tests for Identification of Medical Bacteria. 3rd edition. Lippincott Williams and Wilkins, USA.
Maisuria, V. B.; Hosseinidoust, Z. and Tufenkji, N.( 2015). Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria. Applied and enviro. microbio., 81, 3782-3792.
Medhat, A. R., & Aljanabay, A. A. J. (2022). Epidemiology of typhoid fever in Balad City, Iraq. International Journal of Health Sciences, 6(S1), 1049-1063. https://doi.org/10.53730/ijhs.v6nS1.4834
Mihu, M. R. and Martinez, L. R. (2011). Novel therapies for treatment of multidrug resistant Acinetobacter baumannii skin infections . Virulence. 2(2): 97-102.
Munoz-Price, L. S.; Zembower, T.; Penugonda, S.; Schreckenberger, P.; Lavin, M. A.; Welbel, S.; Vais, D.; Baig, M.; Mohapatra, S.; Quinn, J. P. and Weinstein, R. A. (2010). Clinical Outcomes of Carbapenem-Resistant Acinetobacter baumannii Bloodstream Infections: Study of a 2-State Monoclonal Outbreak. Infect. Control Hosp. Epidemiol. 31(10):18-27
Narten and Maike (2012) "Susceptibility of Pseudomonas aeruginosa urinary tract isolates and influence of urinary tract conditions on antibiotic tolerance." Current microbiology 64.1: 7-16.
Pachon-Ibanez, M. E.; Docobo-Perez, F.; Lopez-Rojas, R., Herrera, J. D.; Mejias, M. E. J.; Curiel, A. G.; Pichardo, C.; Jimenez, L. and Pachon, J. (2010). Efficacy of Rifampin and Its Combinations with Imipenem, Sulbactam, and Colistin in Experimental Models of Infection Caused by Imipenem-Resistant Acinetobacter baumannii . Antimicrob. Agents Chemother. 54(3): 1165-1172.
Shah R. K S 1, Zhao H Ni 1, Xiao Y S.1, Guo Q. W. 1, Fan L. (2017) ; The Determination and Correlation of Various Virulence Genes, ESBL, Serum Bactericidal Effect and Biofilm Formation of Clinical Isolated Classical Klebsiella pneumoniae and Hypervirulent Klebsiella pneumoniae from Respiratory Tract Infected Patients . Article , Pol J Microbiol . 2017 Dec 4;66(4):501-508. doi: 10.5604/01.3001.0010.7042
Sharma, V. K. & Bearson, B. L. 2013. Hha controls Escherichia coli O157: H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD. Appl. Environ. Microbiol., 79, 2384-2396.
Shea, O. MK. (2012). Acinetobacter in modern warfare. Int J Antimicrob Agents. May;, 39(5), 363-75.
Shields, R. K.; Kwak, E. J.; Potoski, B. A.; Doi, Y.; Adams-Haduch, J. M.; Silviera, F. P.; Toyoda, Y.; Pilewski, J. M.; Crespo, M.; Pasculle, A. W.; Clancy, C. J. and Nguyen, M. H. ( 2011). High mortality rates among solid organ transplant recipients infected with extensively drug-resistant Acinetobacter baumannii: using in vitro antibiotic combination testing to identify the combination of a carbapenem and colistin as an effective treatment regimen. Diagn. Microbiol. Infect. Dis. 70(2): 246-252.
Wasfi , R.; Abdellatif, G. R.; Elshishtawy H. M. and Ashour, H. M. (2020) First-time characterization of viable but non-culturable Proteus mirabilis : Induction and resuscitation. J.of cellular and molecular medicine. 12Wen.
Widana, I.K., Sumetri, N.W., Sutapa, I.K., Suryasa, W. (2021). Anthropometric measures for better cardiovascular and musculoskeletal health. Computer Applications in Engineering Education, 29(3), 550–561. https://doi.org/10.1002/cae.22202
Wijayanti, N. (2021). Factors related to behavior the community in disposing of garbage. International Journal of Health & Medical Sciences, 4(1), 74-79. https://doi.org/10.31295/ijhms.v4n1.1226
Yang, H.; Liang, L.; Lin, S. and Jia, S. (2010).Isolationland Characterization of a Virulent Bacteriophage AB1 of Acinetobacter baumannii .BMC Microbiol. 10: 131-141.
Zander, E.; Nemec, A.;` Seifert, H. and Higgins, P. G. (2012). Association between beta-lactamase encoding blaOXA-51 variants and DiversiLab rep-PCR based typing of Acinetobacter baumannii. J. Clin. Microbiol. 50(6): 1900-1904.
Zhong, Q. M. D.; Xu, W. M. D. ; Wu, Y.M. D., and Xu, H. M. D. (2012). Clonal Spread of Carbapenem Non-susceptible Acinetobacter baumannii in an Intensive Care Unit ina Teaching Hospital in China. Ann. Lab. Med. 32(6): 413-419
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