Detection of some virulence factors and antibiotic resistance of Staphylococcus aureus isolated from clinical cases
Keywords:
MRSA, MSSA, β- lactam antibioticsAbstract
Staphylococcus aureus is a gram-positive bacteria that cause a wide variety of clinical diseases, appears as grape like clusters, non-sporing, nonmotile and usually non capsulated bacteria. The current study was carried out from September to December 2021 , in which 61 S. aureus isolated from 150 clinical specimens. It is resistant to most β- lactam antibiotics due to an alteration in the penicillin-binding protein (PBP2a) that is encoded by the mec genes ,during that can be classified as Methicillin-resistant S. aureus (MRSA) that resistant to a wide range of β-lactam antimicrobial agent due to the presence of the mecA gene and methicillin-sensitive S. aureus (MSSA) that usually resistant penicillin due to β-lactamases. antibiotic susceptibility of all 61 S. aureus isolates showed resistant to Amoxicillin/clavulanic and Cefepime (100%) followed by Cefotaxime(94%) , Azithromycin (80%) and Erythromycin (60.6%) while the Vancomycin, Ceftriaxone, Amikacin, Gentamicin and Imipenem antibiotic gives high sensitivity (100%) followed by Meropenem and Levofloxacin(80%). S. aureus contains many genes which encode the most important toxin like the tst gene which encode toxic shock syndrome toxin (TSST-1) and luks gene which encode Panton Valentine leukocidin (PVL) toxin.
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