Phenotypic and genotypic detection of Enterobacter spp isolated from clinical cases

Sara Mohssin Yassir; Basaad Abd Zaid

doi:10.53730/ijhs.v6nS3.7767

Authors

Sara Mohssin Yassir Department of Biology, University of Kufa, Iraq
Basaad Abd Zaid Department of Biology, University of Kufa, Iraq

Keywords:

ESBL, β –lactamases enzymes, Ampc, blaCTX-M

Abstract

Enterobacter Spp an opportunistic bacteria that causes a variety of infections in hospitalized patients around the world, can resist -lactams primarily by generating the CTX-M enzymes and AmpC -lactamase enzyme. The spread of ESBL is a serious public health concern, and infections with ESBL-producing organisms have been linked to negative outcomes. We looked at the prevalence and screening criteria for extended-spectrum-lactamases (ESBLs) in Enterobacter spp. that produce AmpC. The 20 isolates (18 Enterobacter Cloacae Complex,2 Enterobacter Aerogenes) isolated from clinical cases . We performed polymerase chain reaction for Ampc and blaCTX-M. The Ampc was detected in 12 of 20 isolated, and the blaCTX-M was detected in 11 of 20 isolated.

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References

Abbas, Hisham A., et al(2019). "Impact of specific inhibitors on metallo-β- carbapenemases detected in Escherichia coli and Klebsiella pneumoniae isolates." Microbial pathogenesis 132: 266-274.‏

Ahmad, K. M.; Alamen, A. A. M.; Shamsi, S. A. M. S. and Elzen, A. A. (2019). Emergence of AmpC β-Lactamase and Metallo-β-Lactamase producing. M J I W AS. ;27(1): 9-16.‏

Auer, George K., and Douglas B. Weibel. (2017)"Bacterial cell mechanics." Biochemistry 56.29: 3710-3724.‏

Barnaud, G., Labia, R., Raskine, L., Sanson-Le Pors, M.J., Philippon, A., and

Arlet, G.( 2001). Extension of resistance to cefepime and cefpirome associated to a six amino acid deletion in the H-10 helix of the cephalosporinase of an Enterobacter cloacae clinical isolate. FEMS Microbiol. Lett. 195: 185–190.

Brown, A. and Smith, K.,( 2015). Benson's Microbiological Applications, Laboratory Manual in General Microbiology. 13th Ed. pp. 277

Bryskier, A. (2005)"Antibiotics and Antibacterial Agents: Classifications and Structure‐ Activity Relationship." Antimicrobial agents: antibacterials and antifungals : 13-38.‏

Bush, K. and Jacoby ,G.A. (2010): Updated functional classification of beta - lactamases. A AC.; 54(3): 969–976 .

Davin-Regli, A. (2015). E. aerogenes and E. cloacae; versatile bacterial pathogens confronting antibiotic treatment. FM.; 6( 392):1-10.

Dörr, T. (2020). Understanding tolerance to cell wall–active antibiotics. Annals of the New York Academy of Sciences.;2020:1-23.

Kaneko, Kenichi, et al. "Gene mutations responsible for overexpression of AmpC β-lactamase in some clinical isolates of Enterobacter cloacae." Journal of Clinical Microbiology 43.6 (2005): 2955-2958.

Kawai, Akito, et al. (2020) "Structural basis of reduced susceptibility to ceftazidime-avibactam and cefiderocol in Enterobacter cloacae due to AmpC R2 loop deletion." Antimicrobial agents and chemotherapy 64.7: e00198-20.‏

Liu, G.; Bogaj, K.; Bortolaia, V.; Olsen, J. E. and Thomsen, L. E. (2019). Antibiotic-Induced, increased conjugative transfer is common to diverse naturally occurring ESBL plasmids in Escherichia coli. FM.; 10:1-12.

MacFaddin, J.F.(2000). Biochemical tests for identification of medical bacteria .(3rd edition).The Williams and Wilkins-Baltimor. USA.J A M.;6(1) .

Nachimuthu, R.; Kannan, V. R.; Bozdogan, B.; Krishnakumar, V. and Manohar, P. (2020). CTX-M-type ESBL-mediated resistance to third-generation cephalosporins and conjugative transfer of resistance in Gram-negative bacteria isolated from hospitals in Tamil Nadu.A M.;3(3):141-167.

Newire, E.A.; Ahmed, S.F.; House, B.; Valiente, E.; Pimentel, G. (2013) Detection of new SHV-12, SHV-5 and SHV-2a variants of extended spectrum Beta-lactamase in K. pneumoniae in Egypt. A C M and A.: 12:16.1-12.

Sauvage, E.; Kerff, F. ; Terrak, M. ; Ayala, J. A. and P. Charlier. (2008). The penicillin-binding proteins: Structure and role in peptidoglycan biosynthesis. FEMS M R. ;32(2): 234–258.

Sophie, B.; Francois-Xavier ,W.; Axel ,C.; Martine; V.; Eric M and Karine P.; (2006).Clonal Emergence of Extended-Spectrum β-Lactamase (CTX-M-2)-Producing Salmonella enterica Serovar Virchow Isolates with reduced susceptibilities to ciproß oxacin among poultry and humans in Belgium and France (2000 to 2003) J C M .;44: -903- 2897.