Comparative Evaluation of Antimicrobial activity of Tulsi extract [Ocimum Sanctum (Linn.)] and Curcumin extract [Curcuma Longa] with 0.2% Chlorhexidine on Periodontal Pathogens
An in-vitro study
Keywords:
Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Periodontitis, Tulsi extract, Curcumin extract, Ocimum Sanctum, Curcuma Longa, Periodontal disease, Herbal extracts, Zone of inhibitionAbstract
BACKGROUND: Periodontal disease is associated with colonization of specific bacteria in the subgingival area. Widespread use of antimicrobials has resulted in emergence of various side effects and drug resistance. Phytomedicine has been used in many clinical conditions and it appears to be a suitable alternative to manage conditions affecting the oral cavity. AIM: Present study aims to assess the in-vitro antimicrobial efficacy of Tulsi extract and Curcumin extract on Periodontal pathogens. MATERIALS & METHODS: Ethanolic extract of 100% w/v of Tulsi and Curcumin was prepared by cold extraction technique. Prepared extracts were further diluted to obtain solutions of 5 different concentrations to be tested (0.5%, 1%, 2% and 5%). Obtained solutions and controls (0.2% Chlorhexidine and Distilled water) were subjected to the microbiological investigation against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Agar well diffusion method was employed to determine the concentration at which Tulsi and Curcumin gave an inhibition zone, similar to Chlorhexidine. Data was analysed using one‑way analysis of variance and post‑hoc test for inter-group and intra‑group comparisons. RESULTS: At 10% concentration both Tulsi and Curcumin extract have demonstrated comparable antimicrobial activity against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.
Downloads
References
Agarwal P, Nagesh L, Murlikrishnan. Evaluation of the antimicrobial activity of various concentrations of Tulsi (Ocimum sanctum) extract against Streptococcus mutans: An in vitro study. Indian J Dent Res 2010;21:357-9.
Asteriou E, Gkoutzourelas A, Mavropoulos A, Katsiari C, Sakkas L, Bogdanos D. Curcumin for the Management of Periodontitis and Early ACPA-Positive Rheumatoid Arthritis: Killing Two Birds with One Stone. Nutrients. 2018;10(7):908-925.
Balagopal S, Arjunkumar R. Chlorhexidine: The Gold Standard Antiplaque Agent. J Pharm Sci & Res. 2013;5(2):270-274.
Bouziane A, Hamdoun R, Abouqal R, Ennibi O. Global prevalence of aggressive periodontitis: A systematic review and meta-analysis. J Clin Periodontol. 2020;47(4):406-428.
Carranza FA, Newman MG, Takei HH, Klokkevold PR. Carranza’s Clinical Periodontology. 10th -ed. St. Louis. Mo.: Elsevier Saunders; 2006.
Doros K P, Lerche-Sehm J, Abron A, Fine J, Papapanou P. Infection patterns in chronic and aggressive periodontitis. J Clin Periodontol. 2005;32(10):1055- 61.
Gaibullaeva, N. N. (2021). The role of clinical examination early diagnosis of glaucoma. International Journal of Health & Medical Sciences, 4(3), 333-337. https://doi.org/10.31295/ijhms.v4n3.1745
Jayanti I, Mohammed J, Avijeeta A, Ramanna P, Rai P, Nair R. In vitro Antimicrobial Activity of Ocimum sanctum (Tulsi) Extract on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. J Contemp Dent Pract. 2018;19(4):415-419.
Jepsen S, Blanco J, Buchalla W, Carvalho JC, Dietrich T, Dörfer C, Eaton KA, Figuero E, Frencken JE, Graziani F, Higham SM, Kocher T, Maltz M, OrtizVigon A, Schmoeckel J, Sculean A, Tenuta LM, Veen MH, Machiulskiene V. Prevention and control of dental caries and periodontal diseases at individual and population level: consensus report of group 3 of joint EFP/ORCA workshop on the boundaries between caries and periodontal diseases. J Clin Periodontol. 2017;44(18):85-93.
Jo F, Sharma P, Stenhouse L, Green D, Laverty D, Dietrich T. Global epidemiology of dental caries and severe periodontitis - a comprehensive review. J Clin Periodontol. 2017;44(18):94-105.
Kakudate N, Morita M, Fukuhara S, Sugai M, Nagayama M, Kawanami M, Chiba I. Application of self-efficacy theory in dental clinical practice. Oral Dis. 2010;16(8):747-52.
Kinane DF, Stathopoulou PG, Papapanou PN. Periodontal diseases. Nat Rev Dis Primers. 2017;22(3):17038.
Mallikarjun S, Rao A, Rajesh G, Shenoy R, Pai M. Antimicrobial efficacy of Tulsi leaf (Ocimum sanctum) extract on periodontal pathogens: An in vitro study. J Indian Soc Periodontol. 2016;20(2):145-50.
Newman MG, Takei HH, Klokkevold PR, Carranza FA. Carranza’s Clinical Periodontology. 11th -ed. St. Louis. Mo.: Elsevier Saunders;2006.
Rozman U, Pušnik M, Kmetec S, Duh D, Šostar Turk S. Reduced Susceptibility and Increased Resistance of Bacteria against Disinfectants: A Systematic Review. Microorganisms. 2021;9(12):2550.
Shah S, Trivedi B, Patel J. Evaluation and comparison of antimicrobial activity of Tulsi (Ocimum Sanctum), Neem (Azadirachta indica) and triphala extract against Streptococcus. Natl J Int Res Med. 2014;5:17-21.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
Vishwakarma A. A Review on Antimicrobial Potential of Indian Ocimum sanctum (Tulsi). IJSR. 2017;6(5):1490-92.
Westfelt E. Rationale of mechanical plaque control. J Clin Periodontol. 1996;23(3 Pt 2):263-7.
Zorofchian S, Kadir H, Hassandarvish P, Tajik H, Abubakar S, Zandi K. A Review on Antibacterial, Antiviral, and Antifungal Activity of Curcumin. BioMed Res Int. 2014;2014(1):186864.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
