A comparative study of effects of Triphala and Tetracycline gel on root conditioning: A scanning electron microscopic study

Manvi Srivastava; Varsha Rathod; Palak Dwivedi; Antara Parikh

doi:10.53730/ijhs.v6nS4.11404

Authors

Manvi Srivastava
manvisrivastava25@gmail.com
Postgraduate, Department of Periodontics, Bharati Vidyapeeth Dental College, and Hospital
Varsha Rathod Professor and HOD, Department of Periodontics, Bharati Vidyapeeth Dental College, and Hospital
Palak Dwivedi Postgraduate, Department of Periodontics, Bharati Vidyapeeth Dental College and Hospital
Antara Parikh Postgraduate, Department of Periodontics, Bharati Vidyapeeth Dental College and Hospital

Keywords:

tetracycline hydrochloride, triphala, scanning electron microscope, root surface biomodification

Abstract

Background: Remnants of dental calculus, contaminated cementum, and subgingival plaque act as a physical barrier between periodontal tissues and root surfaces, which inhibits the formation of new attachment. Treatment of denuded root surface using various chemicals and antimicrobial agents with scaling and root planing helps in active periodontal regeneration. Aim: The aim of this study was to evaluate and compare the demineralizing effects of Triphala and 1% Tetracycline HCl gel on root conditioning using a Scanning Electron Microscope. Settings and design: A total of 50 hemi-sectioned root samples were obtained from 25 extracted, and root planed samples. These were divided into two groups, Group 1: 1% Tetracycline HCl gel and Group 2: Triphala. Materials and method: The two root conditioning agents were applied with cotton pellets on the sectioned roots. The samples were then examined under the scanning electron microscope. Statistical analysis used: Data obtained was compiled on a MS Office Excel Sheet. Inter group comparison was done using Mann Whitney U test. Comparison of frequencies of categories of variables with groups was done using Chi Square Test. Results: The root conditioning agents used in this study were found to be equally effective in removing the smear layer.

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References

Abraham S, Kumar MS, Sehgal PK, Nitish S, Jayakumar ND. Evaluation of the inhibitory effect of Triphala on PMN-type Matrix Metalloproteinase (MMP-9) J Periodontol. 2005;76:497–502. [PubMed: 15857087] https://doi.org/10.1902/jop.2005.76.4.497

Aleo JJ, De Renzis FA, Farher PA, Varboncoeur AP. The presence biologic activity of cementum bound endotoxin. J Periodontol. 1974;45:672–675. https://doi.org/10.1902/jop.1974.45.9.672

Ashok KP, Shobha PM. A comparative scanning electron microscope study on the effect of acid etching with citric acid, tetracycline hydrochloride and EDTA on instrumented, periodontally involved root surfaces. Indian J Stomatol. 2010;1:616.

Blomlöf JP, Blomlöf LB, Lindskog SF. Smear removal and collagen exposureafter non-surgical root planing followed by etching with an EDTA gel preparation. J Periodontol. 1996;67:841–5.https://doi.org/10.1902/jop.1996.67.9.841

Chahal GS, Chhina K, Chhabra V, Bhatnagar R, Chahal A. Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: ASEM study. J Indian Soc Periodontol. 2014 Jan;18(1):32-7. doi: 10.4103/0972-124X.128196. PMID: 24744541; PMCID: PMC3988639.

Desai A, Anil M, Debnath S. A clinical trial to evaluate the effects of Triphala as a mouthwash in comparison with chlorhexidine in chronic generalized periodontitis patient. Indian J Dent Adv. 2010;2:243–7. https://www.ijdr.in/text.asp?2017/28/1/49/203622

Işik G, Ince S, Sağlam F, Onan U. Comparative SEM study on the effect of different demineralization methods with tetracycline hcl on healthy root surfaces. J Clin Periodontol. 1997;24:589–94. https://doi.org/10.1111/j.1600-051x.1997.tb00234.x

Jagtap AG, Karkera SG. Potential of the aqueous extract of Terminalia chebula as an anticaries agent. J Ethnopharmacol 1999;68:299-306. https://doi.org/10.1016/s0378-8741(99)00058-6

Lafferty TA, Gher ME, Gray JL. Comparative SEM study on the effect of acid etching with tetracycline HCl or citric acid on instrumented periodontally involved human root surfaces. J Periodontol. 1993;64:689–93. [PubMed: 8410605] https://doi.org/10.1902/jop.1993.64.8.689

Lasho DJ, O’Leary TJ, Kafrawy AH. A scanning electron microscope study of the effects of various agents on instrumented periodontally involved root surfaces.Periodontol. 1983;54:210–20. [PubMed: 6406665] https://doi.org/10.1902/jop.1983.54.4.210

Leite FR, Sampaio JE, Zandim DL, Dantas AA, Leite ER, Leite AA. Influence of root-surface conditioning with acid and chelating agents on clot stabilization. Quintessence Int. 2010;41:341–9.

Marshall JS. A remarkable case of pyorrhea alveolaris, with reproduction of bone, occurring in the practice of Dr. Allport, Chicago,IL. JAMA 1883; 1:641-643. doi:10.1001/jama.1883.02390220009001c

Misra V, Mehrotra KK, Dixit J, Maitra SC. Effect of a carbon dioxide laser on periodontally involved root surfaces. J Periodontol. 1999;70:1046–52. [PubMed: 10505808. https://doi.org/10.1902/jop.1999.70.9.1046.

Parveen R, Shamsi TN, Singh G, Athar T, Fatima S. Phytochemical analysis and In-vitro Biochemical Characterization of aqueous and methanolic extract of Triphala, a conventional herbal remedy. Biotechnol Rep (Amst). 2018 Feb

Peterson CT, Denniston K, Chopra D. Therapeutic Uses of Triphala in Ayurvedic Medicine. J Altern Complement Med. 2017 Aug;23(8):607-614. Doi: 1089/acm.2017.0083. Epub 2017 Jul 11. PMID: 28696777; PMCID: PMC5567597.

Rahma, D. Y., & Atmaja, M. H. S. (2022). Peritoneal carcinomatosis and mimicking on CT scan findings. International Journal of Health & Medical Sciences, 5(1), 154-162. https://doi.org/10.21744/ijhms.v5n1.1864

Shewale, A. H., Gattani, D., Mahajan, R., Saravanan, S. P., & Bhatia, N. Root Surface Biomodification: Current Status and a Literature Review on Available Agents for Periodontal Regeneration. Journal of Advances in Medicine andMedical Research, 2015 13(2), 1-14. doi 10.9734/BJMMR/2016/22621

Srikumar R, et al. Evaluation of the growth inhibitory activities of Triphala against common bacterial isolates from HIV infected patients. Phytother Res 2007;21:476–480. https://doi.org/10.1002/ptr.2105

Suryatika, I. B. M., Anggarani, N. K. N., Poniman, S., & Sutapa, G. N. (2020). Potential risk of cancer in body organs as result of torak CT-scan exposure. International Journal of Physical Sciences and Engineering, 4(3), 1–6. https://doi.org/10.29332/ijpse.v4n3.465

Urist MR, Iwata H. Preservation and biodegradation of the morphogenetic property of bone matrix. J Theor Bio. 1966; 38(1):155-67. https://doi.org/10.1016/0022-5193(73)90231-2

Urist MR, Nogami H. Morphogenetic substratum for differentiation of cartilage in tissue culture. Nature. 1970;225:1051–2. [pubmed: 5416473] https://doi.org/10.1038/2251051a0

Vanheusden AJ, Goffinet G, Zahedi S, Nusgens B, Lapiѐre CM, Rompen EH. In vitro stimulation of human gingival epithelial cell attachment to dentin by surface conditioning. J Periodontol. 1999;70:594–603.

Widana, I.K., Sumetri, N.W., Sutapa, I.K., Suryasa, W. (2021). Anthropometric measures for better cardiovascular and musculoskeletal health. Computer Applications in Engineering Education, 29(3), 550–561. https://doi.org/10.1002/cae.22202

Wikesjo UME, Nilveus RE, Selvig KA. Significance of early healing events on periodontal repair: a review. J Periodontol. 1992;63:158–165. https://doi.org/10.1902/jop.1974.45.9.672