Efficacy of biosynthetically developed selenium nanoparticles using plant extracts of clove and cardamom
Keywords:
clove and cardamom, Selenium nanoparticles, antibacterial, anti inflammatory, cytotoxicity, TEMAbstract
Background: The green approachable metal nanoparticles are treated to be an eco-friendly path and cost-effectiveness. In this present study, nano selenium was synthesized profitably by clove and cardamom. Methods: Green synthesis of selenium nanoparticles was preliminarily confirmed by color changing from dark orange to red color in the reaction mixture. The resulting constructed nanoparticles were characterized by using ultraviolet spectroscopy absorbance around 450 nm. Characterization using HRTEM (High Resolution- Transmission Electron Microscope) to identify its size and shape. The organisms used to assess the antimicrobial efficacy of clove and cardamom reinforced selenium nanoparticles were Streptococcus mutans, Staphylococcus aureus and lactobacillus species and Candida albicans using Agar well diffusion method at 25 µL, 50 µL and 100 µL. In addition, we have analyzed its anti-inflammatory and cytotoxicity activity. Results: UV-VIS spectroscopic analysis which showed a peak at 450 nm of the visible spectrum by reduction of selenium salts to Se NP’s. TEM spherical shape was of size 5 -20 nm. Good antimicrobial activity was noted against Streptococcus mutans, Staphylococcus aureus, and Lactobacillus species and Candida albicans. The mean zone of inhibition was found to be increased as the concentrations of Se NPs increased.
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