Cytotoxic effects of fresh aloe vera gel mediated silver nano composite: An In vitro study

Soya Alfred Xavier; Abdul Wahab; S Rajeshkumar

doi:10.53730/ijhs.v6nS4.9951

Authors

Soya Alfred Xavier Post Graduate Department of Oral and Maxillofacial Surgery Saveetha Dental College Saveetha Institute of Medical and Technical Science, Saveetha University Chennai, India 600077
Abdul Wahab Professor Department of Oral and Maxillofacial Surgery Saveetha Dental College Saveetha Institute of Medical and Technical Science, Saveetha University Chennai, India- 600077
S Rajeshkumar Associate Professor Nanobiomedicine Lab Saveetha Dental College Saveetha Institute of Medical and Technical Science, Saveetha University Chennai, India 600077

Keywords:

silver nano particle, aloe vera , nano medicine, silver nitrate, nano technology

Abstract

Aim: To evaluate the cytotoxic effect if fresh aloevera gel mediated silver nano composites Materials And Methods: A 10 gram portion of thoroughly washed fresh cut aloe vera was used in this study. 10 gram of the pulp of the leaf was extracted and was used for the experiments. 10 gram of silver nitrate powder was dissolved in 40 ml of distilled water. To this 40 ml of distilled water the fresh aloe vera extract was added. The solution was placed in the shaker and an observation was made for every two hours for analysing the synthesis of nanoparticles under UV visual spectroscopy . This was followed by the placement of the solution onto the magnetic stirrer for the formation of the nanoparticle .The mixture is then centrifuged at 8000 rpm for 10 minutes and then the nano particles were collected .The resulting solution was further characterised by the TEM measurements. The cytotoxicity was tested by seeding brine shrimps into a 6 well plate. Various concentration of silver nitrate solution was added on the mixture.

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References

Chandran, S. P., M. Chaudhary, R. Pasricha, A. Ahmad, and M. Sastry. 2006. “Synthesis of Gold Nanotriangles and Silver Nanoparticles Using Aloe Vera Plant Extract.” Biotechnology Progress. https://doi.org/10.1021/bp0501423.

Kanniah P, Radhamani J, Chelliah P, Muthusamy N, Joshua Jebasingh Sathiya Balasingh Thangapandi E, Reeta Thangapandi J, Balakrishnan S, Shanmugam R. Green Synthesis of Multifaceted Silver Nanoparticles Using the Flower Extract of Aerva lanata and Evaluation of Its Biological and Environmental Applications. Chemistry Select. 2020 Feb 21 ; 5 (7) :2322 - 2331. https://doi.org/10.1002/slct.201903228.

Malarkodi C, Rajeshkumar S, Paulkumar K, Gnanajobitha G, Vanaja M, Annadurai Bacterial synthesis of silver nanoparticles by using optimized biomass growth of Bacillus sp. G (2013) Nanoscience and nanotechnology – An international Journal 3(2): 26-32.

Mukherjee, Priyabrata, Absar Ahmad, Deendayal Mandal, Satyajyoti Senapati, Sudhakar R. Sainkar, Mohammad I. Khan, Renu Parishcha, et al. 2001. “Fungus-Mediated Synthesis of Silver Nanoparticles and Their Immobilization in the Mycelial Matrix: A Novel Biological Approach to Nanoparticle Synthesis.” Nano Letters. https://doi.org/10.1021/nl0155274.

Park, Ji-Ho, Luo Gu, Geoffrey von Maltzahn, Erkki Ruoslahti, Sangeeta N. Bhatia, and Michael J. Sailor. 2009. “Biodegradable Luminescent Porous Silicon Nanoparticles for in Vivo Applications.” Nature Materials 8 (4): 331–36.

Paulkumar K, G Gnanajobitha, M Vanaja, Shunmugam Rajeshkumar, C Malarkodi and G Annadurai Biosynthesis of silver chloride nanoparticles using Bacillus subtilis MTCC 3053 and assessment of its antifungal activity (2013) ISRN Nanomaterials doi.org/10.1155/ 2013/ 317963, 1-8.

Rajeshkumar S and C Malarkodi, (2014) In vitro antibacterial activity and mechanism of silver nanoparticles against food borne pathogens Bioinorganic Chemistry and Applications http://dx.doi.org/10.1155/2014/581890.

Rajeshkumar S, Malarkodi C, Vanaja M, , Annadurai G Anticancer and enhanced antimicrobial activity of biosynthesizd silver nanoparticles against clinical pathogens Journal of Molecular Structure 1116 (2016) 165-173.

Rajeshkumar S. Antioxidant activity of characterized silver nanoparticles synthesized using flower extracts of Chrysanthemum indicum Research Journal of Biotechnology (2017) 12 (8): 38-43.

Rajeshkumar S. Phytochemical constituents of fucoidan (Padina tetrastromatica) and its assisted AgNPs for enhanced antibacterial activity. IET nanobiotechnology. 2016 Jul 26;11(3):292-299 doi: 10.1049/iet-nbt.2016.0099

Rinartha, K., & Suryasa, W. (2017). Comparative study for better result on query suggestion of article searching with MySQL pattern matching and Jaccard similarity. In 2017 5th International Conference on Cyber and IT Service Management (CITSM) (pp. 1-4). IEEE.

Rinartha, K., Suryasa, W., & Kartika, L. G. S. (2018). Comparative Analysis of String Similarity on Dynamic Query Suggestions. In 2018 Electrical Power, Electronics, Communications, Controls and Informatics Seminar (EECCIS) (pp. 399-404). IEEE.

Stoimenov, Peter K., Rosalyn L. Klinger, George L. Marchin, and Kenneth J. Klabunde.

Swapna Sreenivasagan, Aravind Kumar Subramanian and S. Rajeshkumar (2020). Assessment of antimicrobial effects and cytotoxic effect of green mediated silver nanoparticles and its coating onto mini-implants. Ann. Phytomed., 9(1):207-212. http://dx.doi.org/10.21276/ap.2020.9.1.27

Uludağ, Yıldız, and Ibtisam E. Tothill. 2010. “Development of a Sensitive Detection Method of Cancer Biomarkers in Human Serum (75%) Using a Quartz Crystal Microbalance Sensor and Nanoparticles Amplification System.” Talanta. https://doi.org/10.1016/j.talanta.2010.04.034.

Venkat Kumar S Rajeshkumar S Optimized production of silver nanoparticles using marine macroalgae Sargassum myriocystum for its anticancer and enhanced antibacterial activity Journal of Bionanoscience (2017) 1: 1-7 doi:10.1166/jbns.2017.1458.