Comparative evaluation of antimicrobial activity of newer herbal disinfectant formulations on hospital microbial flora

Akkina Rajani Chowdary; Indugula Jyothi Padmaja; Syam Kumar Addepalli; Payala Vijayalakshmi

doi:10.53730/ijhs.v6nS8.12516

Authors

Akkina Rajani Chowdary Assistant Professor, Department of Microbiology and Food Science & Technology, GITAM School of Science, GITAM (Deemed to be University), Rushikonda, Visakhapatnam-530045, Andhra Pradesh, India
Indugula Jyothi Padmaja Professor and Principal, GITAM Institute of Medical sciences and Research, GITAM (Deemed to be University), Rushikonda, Visakhapatnam-530045, Andhra Pradesh, India
Syam Kumar Addepalli Assistant professor, Department of Pharmacology, GITAM Institute of Medical sciences and Research, GITAM (Deemed to be University), Rushikonda, Visakhapatnam-530045, Andhra Pradesh, India
Payala Vijayalakshmi
bavisettyvijayalakshmi2@gmail.com
Assistant professor, Department of Microbiology, GITAM Institute of Medical sciences and Research, GITAM (Deemed to be University), Rushikonda, Visakhapatnam-530045, Andhra Pradesh, India

Keywords:

antimicrobial activity, plant extracts, disinfectants, microorganisms, hospital wards

Abstract

Background: The world is now focusing on use of herbals because they are eco-friendly and less toxic on human health and environment Objectives:To evaluate antimicrobial activity of newer herbal disinfectant formulations on hospital microbial flora Methods: Herbal disinfectants were prepared with medicinal plant leaf extracts of Tridax procumbens, Withania somnifera, Tinospora cordifolia, Costus igneus and Nepata cataria. Hospital microbial flora was isolated by rubbing and rotating sterile swabs from targeted spots. Extract formulations were tested for the antimicrobial activity against five isolatedbacterial strains i.e., Escherichia coli, Pseudomonas aeruginosa, Enterobacter sp., Enterococcus faecalis and Staphylococcus aureus using Kirby-bauer disc diffusion method. Results: Extracts exhibited antimicrobial activity against all tested microorganisms. Among the five different herbal disinfectants, Tinospora cordifolia showed good antimicrobial activity against Escherichia coli, Enterobacter and Enterococcus faecalis with 27mm, 20mm and 14mm of zone of inhibition respectively. Costus igneus showed high antimicrobial activity to Pseudomonas aeruginosa with 22mm of zone of inhibition. The herbal disinfectant formulations in the present study were reported effective results against hospital microbial flora which cause nosocomial infections Conclusions: Herbal disinfectants Tinospora cordifolia, Costus igneus, Withania somnifera exhibits antimicrobial activities against MDR bacterial pathogens isolated from hospital wards.

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