Phenotypic and genotypic characterization of macrolide resistance in Staphylococcus aureus isolates from wound infection
Keywords:
D- test, erm genes, MRSAAbstract
Microbiologists are increasingly concerned about the rise in S. aureus MLSB (macrolide, lincosamide, streptogramin B) drug resistance. Clindamycin has been effective in treating infections by S. aureus, and the variations in clindamycin sensitivity patterns cause treatment to fail. Inducible clindamycin resistance in S. aureus is expressed via erythromycin ribosome methylase genes. In the current study, 25 S. aureus isolates were identified by conventional chemical tests and the Vitek®2 system. Specific primers were used for the amplification of Macrolide genes by PCR. Among 25 S. aureus isolates, 23(92%) isolates were methicillin resistant and 2(8%) isolates were methicilin sensitive. The 5(20%) isolates showed resistance to Erythromycin and sensitivity to Clindamycin with a positive D test which was identified as inducible MLSB, while 2(8%) isolates showed resistance criteria for both Erythromycin and Clindamycin which identity as a constitutive MLSB and 18(92%) isolates were given the sensitivity for both Erythromycin and Clindamycin. The erm resistance genes (ermA, ermB, ermC, ermF, and ermG) were detected in 5(20%), 17(68%), 25(100%), 24(96%),11(44%) respectively. The D-test, and Vitek ®2 system should be routinely done to avoid treatment failure due to clindamycin resistance.
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AlAl-Amara,S.,S.,M.(2022).,Constitutive and Inducible ClindamycinResistance Frequencies amongStaphylococcus sp. Coagulase Negative Isolatesin Al-Basrah Governorate, Iraq ,Rep. Biochem. Mol. Biol, 11(1):31-35
Anon, Sneh Lata, Rosy Bala, Neerja Jindal, and Nitin Gupta. 2020. “In Vitro Study of Constitutive and Inducible Clindamycin Resistance in Staphylococcus Aureus with Reference to Methicillin Resistant Staphylococcus Aureus: Experience From Tertiary Care Hospital in Punjab.” Indian Journal of Public Health Research & Development 11(2): 314.
Arjyal, Charu, Jyoti Kc, and Shreya Neupane. 2020. “Prevalence of Methicillin-Resistant Staphylococcus Aureus in Shrines.” International Journal of Microbiology 2020.
Bazzi, Ali M, Ali A Rabaan, Mahmoud M Fawarah, and Jaffar A Al-Tawfiq. 2017. “Direct Identification and Susceptibility Testing of Positive Blood Cultures Using High Speed Cold Centrifugation and Vitek II System.” Journal of Infection and Public Health 10(3): 299–307.
Bhomi, Ujwol et al. 2016. “Status of Inducible Clindamycin Resistance among Macrolide Resistant Staphylococcus Aureus.” African Journal of Microbiology Research 10(9): 280–84.
Chen, Jing et al. 2007. “Development and Application of Real-Time PCR Assays for Quantification of Erm Genes Conferring Resistance to Macrolides-Lincosamides-Streptogramin B in Livestock Manure and Manure Management Systems.” Applied and Environmental Microbiology 73(14): 4407–16.
CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty - Second Informational Supplement. CLSI document M100 -S22. Wayne, PA: Clinical and Laboratory Standards Institute; 2012 .
Djunaid, R., Sahar, J., Widyatuti, W., & Priyo, S. (2022). Predictive factors contributing to violent behavior in adolescents . International Journal of Health Sciences, 6(2), 1148–1159. https://doi.org/10.53730/ijhs.v6n2.11483
Elsayed, Mohamed Sabry Abd Elraheam et al. 2019. “Phenotypic and Genotypic Methods for Identification of Slime Layer Production, Efflux Pump Activity, and Antimicrobial Resistance Genes as Potential Causes of the Antimicrobial Resistance of Some Mastitis Pathogens from Farms in Menoufia, Egypt.” Molecular Biology Reports 46(6): 6533–46. https://doi.org/10.1007/s11033-019-05099-6.
Fasihi, Yasser, Fereshteh Saffari, Mohammad Reza Kandehkar Ghahraman, and Davood Kalantar-Neyestanaki. 2017. “Molecular Detection of Macrolide and Lincosamide-Resistance Genes in Clinical Methicillin-Resistant Staphylococcus Aureus Isolates from Kerman, Iran.” Archives of Pediatric Infectious Diseases 5(1): 4–8.
Ferreira, Carolina et al. 2021. “Clonal Lineages, Antimicrobial Resistance, and Pvl Carriage of Staphylococcus Aureus Associated to Skin and Soft-Tissue Infections from Ambulatory Patients in Portugal.” Antibiotics 10(4).
Ghanbari, Fahimeh et al. 2016. “Distribution of Erm Genes among Staphylococcus Aureus Isolates with Inducible Resistance to Clindamycin in Isfahan, Iran.” Advanced Biomedical Research 5(1): 62.
Goudarzi, Mehdi et al. 2019. “Clonal Dissemination of Staphylococcus Aureus Isolates Causing Nosocomial Infections, Tehran, Iran.” Iranian Journal of Basic Medical Sciences 22(3): 238–45.
Grgičević, Ivan et al. 2020. “Discovery of Macrozones, New Antimicrobial Thiosemicarbazone-Based Azithromycin Conjugates: Design, Synthesis and in Vitro Biological Evaluation.” International Journal of Antimicrobial Agents 56(5).
Heyar, Avneet Kaur, Kamaldeep Kaur, Amarjit Kaur Gill, and Prabhjot Kaur Gill. 2020a. “Induction of Clindamycin Resistance in Clinical Isolates of Staphylococcus Aureus From a Tertiary Care Hospital.” International Journal of Medical and Biomedical Studies 4(12).
Institute, CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty - Third Informational Supplement. 28th edn. Edited by P. Wayne. Wanye, PA Clinical and Laboratory Standards Institute Antimicrobial, 2018.
Jha, Awadhesh Kumar et al. 2019. “Determine Constitutive and Inducible Clindamycin Resistance among Clinical Isolates of Staphylococcus Aureus Isolates from Tertiary Care Hospital, Bettiah, India.” International Journal of Contemporary Medical Research [IJCMR] 6(11): 1–5.
Keenan, Jeremy D. et al. 2019. “Longer-Term Assessment of Azithromycin for Reducing Childhood Mortality in Africa.” New England Journal of Medicine 380(23): 2207–14.
Khashei, Reza, Yalda Malekzadegan, Hadi Sedigh Ebrahim-Saraie, and Zahra Razavi. 2018. “Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Isolates of Staphylococci in Southwest of Iran.” BMC Research Notes 11(1): 1–6.
Khoshnood, Saeed et al. 2019a. “Distribution of Genes Encoding Resistance to Macrolides, Lincosamides, and Streptogramins among Methicillin-Resistant Staphylococcus Aureus Strains Isolated from Burn Patients.” Acta Microbiologica et Immunologica Hungarica 66(3): 387–98.
Koike, Satoshi et al. 2010. “Molecular Ecology of Macrolide-Lincosamide-Streptogramin B Methylases in Waste Lagoons and Subsurface Waters Associated with Swine Production.” Microbial Ecology 59(3): 487–98.
Kow, Chia Siang, and Syed Shahzad Hasan. 2020. “Use of Azithromycin in COVID 19: A Cautionary Tale.” Clinical Drug Investigation 40(10): 989–90. https://doi.org/10.1007/s40261-020-00961-z.
Lina, Gerard et al. 1999a. “Distribution of Genes Encoding Resistance to Macrolides, Lincosamides, and Streptogramins among Staphylococci.” Antimicrobial Agents and Chemotherapy 43(5): 1062–66.
Modukuru, Giridhar Kumar, Pradeep Madala Sobhana Surya, Vishnuvardhana Rao Kakumanu, and Saritha Yarava. 2021. “Phenotypic Characterization of Macrolide-Lincosamide-Streptogramin B Resistance in Staphylococcus Aureus.” Journal of Pure and Applied Microbiology 15(2): 689–94.
Mokta, Kiran K. et al. 2015. “Inducible Clindamycin Resistance among Clinical Isolates of Staphylococcus Aureus from Sub Himalayan Region of India.” Journal of Clinical and Diagnostic Research 9(8): DC20–23.
Moosavian, Mojtaba et al. 2014. “Inducible Clindamycin Resistance in Clinical Isolates of Staphylococcus Aureus Due to Erm Genes, Iran.” Iranian Journal of Microbiology 6(6): 421–27.
Nezhad, Ramin Rashidi et al. 2017. “Molecular Characterization and Resistance Profile of Methicillin Resistant Staphylococcus Aureus Strains Isolated from Hospitalized Patients in Intensive Care Unit, Tehran-Iran.” Jundishapur Journal of Microbiology 10(3).
Nikam, Archana P., Pramod R. Bhise, and Mukta M. Deshmukh. 2017. “Phenotypic Detection of Inducible Clindamycin Resistance among Staphylococcus Aureus Isolates.” International Journal of Research in Medical Sciences 5(2): 543.
Papkou, Andrei et al. 2020. “Efflux Pump Activity Potentiates the Evolution of Antibiotic Resistance across S. Aureus Isolates.” Nature Communications 11(1). http://dx.doi.org/10.1038/s41467-020-17735-y.
Sarrou, Stela et al. 2019. “MLSB-Resistant Staphylococcus Aureus in Central Greece: Rate of Resistance and Molecular Characterization.” Microbial Drug Resistance 25(4): 543–50.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
Widana, I. K., Sumetri, N. W., & Sutapa, I. K. (2018). Effect of improvement on work attitudes and work environment on decreasing occupational pain. International Journal of Life Sciences, 2(3), 86–97. https://doi.org/10.29332/ijls.v2n3.209
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