Immunohistochemical analysis of CD34 and PCNA expression in salivary and laryngeal adenoid cystic carcinoma

Samia El-Azab; Emad Alqalshy; Kamal Abd El-Rahman; Ahmed Hussein; Amr Ibrahim; Magdy Alabasiry Alazzazi; Amr Saad Abdel-Wahab; Hany Mahmoud Mahmoud; Wael Badawy Abdelhameed; Abd Elnasser Abd El Mola Esmail

doi:10.53730/ijhs.v5nS2.12761

Authors

Samia El-Azab Department of Oral Pathology, Galala University; Egypt, and Department of Oral Pathology, Faculty of Dentistry, Cairo University, Egypt
Emad Alqalshy
emadalqalshy.209@azhar.edu.eg
Department of Oral and Dental Pathology Department, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Kamal Abd El-Rahman Department of Oral and Dental Pathology Department, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Ahmed Hussein Department of Oral and Dental Pathology department, Faculty of Dental Medicine, Al-Azhar University, Assiut, Egypt
Amr Ibrahim Department of Oral and Dental Pathology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt, and Department of Oral Pathology, Faculty of Dentistry, Deraya University, Minya, Egypt
Magdy Alabasiry Alazzazi Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt, and College of Dentistry, The Islamic University, Najaf, Iraq
Amr Saad Abdel-Wahab Department of Oral and Dental Pathology Department, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Hany Mahmoud Mahmoud Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Wael Badawy Abdelhameed Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Assiut, Egypt
Abd Elnasser Abd El Mola Esmail Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt

Keywords:

adenoid cystic carcinoma, laryngeal adenoid cystic carcinoma, salivary adenoid cystic carcinoma, SACC, LACC, CD34, PCNA

Abstract

Background/purpose: Adenoid cystic carcinomas (ACC) are malignant tumors that affect major and minor salivary glands. LACC is an extremely rare location for this type of tumour. This study describes the immunohistochemical analysis of CD34 and PCNA expression in salivary and laryngeal ACC. Materials and methods: The study included 40 formalin-fixed paraffin-embedded blocks were used, 20 of them were previously diagnosed as SACC, and 20 as LACC. All sections were stained with hematoxylin and eosin (H&E) and immunohistochemical marker to detect CD34 and PCNA protein. Results: Regarding the expression of CD34, SACC had recorded the highest mean area % (31.58), while LACC recorded the lowest mean area % (14.25) and the comparison revealed that there was a highly significant difference between these lesions where P value was (<0.001) concerning the expression of PCNA, SACC had recorded the highest mean area % (36.28), while LACC recorded the lowest mean area % (20.7) and the comparison revealed that there was a highly significant difference between these lesions where P value was (<0.001). Conclusions: immunohistochemical analyses presented here indicated that LACC tends to nonaggressive oncological behavior and angiogenesis when compared to SACC.

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