Immunohistochemical evaluation of CD31 and D2-40, expression in oral squamous cell carci-noma

Emad Alqalshy; Kamal Abd El-Rahman; Amr Ibrahim; Magdy Alabasiry Alazzazi; Amr Saad Abdel-Wahab; Hany Mahmoud Mahmoud; Wael Badawy Abdelhameed; Abd Elnasser Abd El Mola Esmail

doi:10.53730/ijhs.v5nS2.12762

Authors

Emad Alqalshy
emadalqalshy.209@azhar.edu.eg
Department of Oral and Dental Pathology Department, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Kamal Abd El-Rahman Department of Oral and Dental Pathology Department, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Amr Ibrahim Department of Oral and Dental Pathology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt, and Department of Oral Pathology, Faculty of Dentistry, Deraya University, Minya, Egypt
Magdy Alabasiry Alazzazi Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt , and College of Den-tistry, The Islamic University, Najaf, Iraq
Amr Saad Abdel-Wahab Department of Oral and Dental Pathology Department, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Hany Mahmoud Mahmoud Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt
Wael Badawy Abdelhameed Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Assiut, Egypt
Abd Elnasser Abd El Mola Esmail Department of Oral biology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt

Keywords:

immunohistochemistry, CD31, D2-40, tumor angiogenesis, OSSC

Abstract

Background: The present study was carried out to evaluate the relation between angiogenesis, and tumor stage of oral squamous cell carcinoma (OSCC).Materials and methods: Thirty formalin-fixed paraffin embedded blocks were used, ten of them were previously diagnosed as well differentiated OSCC, ten moderately differentiated OSCC and ten poorly differentiated OSCC. To determine the expression of CD31 and D2-40 proteins, streptavidin-biotin immunoperoxidase staining technique was used. The areas with the most vascular density (hot spots) were determined. The stained vessels were counted independently in intratumoral and peritumoral stroma. Results: CD 31 protein showed positive expression in the peritumoral and intratumoral blood vessels subjacent to the malignant invading nests. D2-40 expression was positive in lymphatic vessel in the peritumoral and intratumoral stroma subjacent to the invading nests. The highest mean values of both CD31 and D2-40 proteins expression were recorded in poor differentiated OSSC followed by moderate differentiated OSSC then well differentiated OSSC. There was statistically significant difference found between the three studied groups regarding CD31 and D2-40 levels. Also there was statistically significant positive correlation found between CD31 level and D2-40 level.Conclusion: CD31 and D2-40 are related to stage of OSCC and are consistent with angiogenesis in tumor progression.

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