Immunohistochemical evaluation of CD31 and D2-40, expression in oral squamous cell carci-noma
Keywords:
immunohistochemistry, CD31, D2-40, tumor angiogenesis, OSSCAbstract
Background: The present study was carried out to evaluate the relation between angiogenesis, and tumor stage of oral squamous cell carcinoma (OSCC).Materials and methods: Thirty formalin-fixed paraffin embedded blocks were used, ten of them were previously diagnosed as well differentiated OSCC, ten moderately differentiated OSCC and ten poorly differentiated OSCC. To determine the expression of CD31 and D2-40 proteins, streptavidin-biotin immunoperoxidase staining technique was used. The areas with the most vascular density (hot spots) were determined. The stained vessels were counted independently in intratumoral and peritumoral stroma. Results: CD 31 protein showed positive expression in the peritumoral and intratumoral blood vessels subjacent to the malignant invading nests. D2-40 expression was positive in lymphatic vessel in the peritumoral and intratumoral stroma subjacent to the invading nests. The highest mean values of both CD31 and D2-40 proteins expression were recorded in poor differentiated OSSC followed by moderate differentiated OSSC then well differentiated OSSC. There was statistically significant difference found between the three studied groups regarding CD31 and D2-40 levels. Also there was statistically significant positive correlation found between CD31 level and D2-40 level.Conclusion: CD31 and D2-40 are related to stage of OSCC and are consistent with angiogenesis in tumor progression.
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