Administration of botulinum toxin a and vascular endhotelial growth factor reduce norepinephrine levels and increase viability of modified mcfarlane flap in wistar rat

I Gusti Ayu Putri Purwanthi; Agus Roy Rusly Hariantana Hamid; Tjokorda Gde Bagus Mahadewa; I Made Suka Adnyana; I Gusti Putu Hendra Sanjaya; Sianny Herawati; Gede Wara Samsarga

doi:10.53730/ijhs.v6nS7.13618

Administration of botulinum toxin a and vascular endhotelial growth factor reduce norepinephrine levels and increase viability of modified mcfarlane flap in wistar rat

https://doi.org/10.53730/ijhs.v6nS7.13618

Authors

I Gusti Ayu Putri Purwanthi
purwanthiputri@gmail.com
Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine, Udayana University/ Prof Dr I.G.N.G Ngoerah General Hospital Denpasar, Bali, Indonesia, 80114
Agus Roy Rusly Hariantana Hamid Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine, Udayana University/ Prof Dr I.G.N.G Ngoerah General Hospital Denpasar, Bali, Indonesia, 80114
Tjokorda Gde Bagus Mahadewa Division of Neurospine, Department of Neurosurgery, Faculty of Medicine, Udayana University/ Prof Dr I.G.N.G Ngoerah General Hospital Denpasar, Bali, Indonesia, 80114
I Made Suka Adnyana Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine, Udayana University/ Prof Dr I.G.N.G Ngoerah General Hospital Denpasar, Bali, Indonesia, 80114
I Gusti Putu Hendra Sanjaya Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine, Udayana University/ Prof Dr I.G.N.G Ngoerah General Hospital Denpasar, Bali, Indonesia, 80114
Sianny Herawati Department of Clinical Pathology, Faculty of Medicine, Udayana University/ Prof Dr I.G.N.G Ngoerah General Hospital Denpasar, Bali, Indonesia, 80114
Gede Wara Samsarga Division of Plastic Reconstructive and Aesthetic Surgery, Department of Surgery, Faculty of Medicine, Udayana University/ Prof Dr I.G.N.G Ngoerah General Hospital Denpasar, Bali, Indonesia, 80114

Keywords:

botulinum toxins, VEGF, norepinephrine, microsurgery, flap

Abstract

Local hyperadrenergic states after a random flap elevation mediated by humoral vasoconstrictors can lead to flap necrosis. This study aimed to evaluate the effect of Botulinum Toxin A (BoNT-A) on reducing levels of Norepinephrine (NE) as the main humoral vasoactive substance, as well as its additive effect with Vascular Endothelial Growth Factor (VEGF) in increasing the viability of random flap. 28 Wistar Rats with Caudally Based Modified McFarlane Flap were divided into four groups. Group I received BoNT-A 3 days before flap elevation, group II received VEGF immediately after flap elevation, group III received a combination of BoNT-A and VEGF, group IV was a control group. Result in this study NE levels lowest was found in the BoNT-A+VEGF combination group (94.63 ± 5 .25 ng/ml, p<0.05 in group I, p<0.01 in groups II and IV). The highest value of FVR was shown in the BoNT-A+VEGF combination group (93.37 ± 2.19 %, p < 0.05 in group I, p < 0.01 in groups II and IV). Meanwhile, the increase in flap viability in the VEGF group was not statistically significant. So Conclusion administration of BoNT-A in combination with VEGF can increase the viability of random flaps by decreasing NE levels.

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Published

10-11-2022

How to Cite

Purwanthi , I. G. A. P., Hamid, A. R. R. H., Mahadewa, T. G. B., Adnyana, I. M. S., Sanjaya, I. G. P. H., Herawati, S., & Samsarga, G. W. (2022). Administration of botulinum toxin a and vascular endhotelial growth factor reduce norepinephrine levels and increase viability of modified mcfarlane flap in wistar rat. International Journal of Health Sciences, 6(S7), 6412–6428. https://doi.org/10.53730/ijhs.v6nS7.13618

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Issue

Vol. 6 No. S7 (2022)

Section

Peer Review Articles

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