Antioxidant and hepatoprotective activities of Pongamia pinnata (PP) Linn. leaves on anti-tubercular medicines (isoniazid & rifampin) induced hepatotoxicity in Wistar rats
Keywords:
antioxidant parameters, anti-TB medicines, hepatoprotective activity, liver biomarkers, Pongamia pinnataAbstract
Objectives: -The goal of this study was to see if an aqueous extract of Pongamia Pinnata Linn. (APP) leaves could protect against the hepatotoxicity caused by anti-TB medications (ATM). Methods: -In order to cause hepatotoxicity in rats, anti-tubercular medications were utilised. Silymarin (100 mg/kg p.o.) be present utilised by means of the control medication. For 21 days, an aqueous extract of Pongamia Pinnata Linn. leaves (200 & 400 mg/kg p.o.) was given with anti-TB medicines given one hour before to the aqueous extract. Results: -Biomarker enzymes found in the liver are SGPT, SGOT, ALP, Total Bilirubin & total cholesterol remained raised on anti-TB medicines management. The management of aqueous extract of Pongamia Pinnata Linn.leaves200 mg/kg & 400 mg/kg with anti - TB medicines were considerably decreased biomarker enzymes found in the liver. Antioxidant factors like Super Oxide Dismutase (SOD), Glutathione(GSH), Catalase(CAT), Glutathione Peroxidase (GPx)& Glutathione Reductase(GRx) were inhibited and improved ThioBarbituric Acid Reactive Substances (TBARs)points in anti-TB medicines administration then returned these antioxidant points in the management of aqueous extract of Pongamia Pinnata Linn. Leavesatadosageof 200mg/kg& 400mg/kg. Conclusion: - According to the findings of this research, Pongamia Pinnata Linn. leaves have a protective consequence against hepatotoxicity caused through anti-TB medicines.
Downloads
References
Robbins J, Fleurentin C, Hefler A. Hepatoprotective properties ofcrepisrueAPPelliandanisotesTrisules.Journalofethanopharmacology.,76;2003:105-111.
AriasPS,BesseyAA,LoweryDMandBrockM.Lipidperoxidation Biochemistry measurement and significance in livercellinjury.Indian.J.Exp.Biol.,45;1989:87-89.
MichealeP,CynthiyaJu.MechanismofDruginducedliverinjury. TheAAPSJournal.,1;2006:48-54.
SrivastavaA.,MaggsJ.L.,AntoineD.J.,WilliamsD.P.,SmithD.A.,Park B.K. Role of reactive metabolites in drug-induced hepatotoxicity. Handb. Exp.Pharmacol. 2010;196:165-194.
Prince M.I., Burt A.D., Jones D.E.J. Hepatitis and liver dysfunction withrifampicin therapy for pruritus in primary biliary cirrhosis. Gut 2002; 50:436–439.
Cancado E.L.R., Leitao R.M.C., Carrilho F.J., Laudanna A.A. Unexpectedclinicalremissionofcholestasisafterrifampicintherapyinpatientswithnormal or slightly increased levels of gamma-glutamyltranspeptidase. Am. J.Gastroenterol. 1998;93:1510–1517.
Sharma S.K. Antituberculosis drugs and hepatotoxicity. Infect. Genet. Evol.2004;4:167-170.
DhimanRKandChawlaYK.Digestive DiseasesandSciences2005;10:1807–1812.
CalixtoJB.BrazJ MedBiolRes2000;33(2):179-189.
Kamboj VP.CurrSci2000;78:35-39.
DeSmetPAGM. Drugs1997;54:801-840.
Krishnamurthi A. 1969. The Wealth of India, vol. VIII. Publication and Information Directorate CSIR, New Delhi, India
Meera B., Kumar S., Kalidhar S.B., 2003. A review of the chemistryand biological activity of Pongamia pinnata. J. Med. Arom. Plant Sci. 25, 441 65.
Punitha R., Manohar, S., 2006. Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. J. Ethnopharmacol. 105, 39 46.
Brijesh, S., Daswani, P.G., Tetali, P., 2006. Studies on Pongamia pinnata (L.) Pierre leaves: understanding the mechanism(s) of action in infectious diarrhea. J Zhejiang Univ. Sci. B. 7(8), 665 674
Shoba G.F. and Thomas M., 2001. Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea. J. Ethnopharmacol. 76(1), 73 76.
ChopdeVV, TankarAN,PandeVV, TekadeAR,GowekarNM, BhandariSR,andKhandake,InternationalJournalofGreenPharmacy2008; 72-75.
OECD guidelines for the testing of chemicals revised draft guideline 423:acuteoraltoxicity: Paris:OECD:2000.
KakkarP,DasBandViswanathanPN.AmodifiedspectrophotometricassayofSOD.IndianJournalofBiochemistryBiophysics1984; 2:130-132.
Aebi H. Catalase in vitro. Methods in Enzymology 1984: 105:121-126.
Sener G, Sehirli AO, Gedik N and Dulger GA. Rosiglitazone, aPPAR-?ligand,protectsagainstburn-inducedoxidativeinjuryofremote organs.Burns2007; 33:587-593.
Paglia DE and Valentine WN. Studies on the quantitative and qualitative characterization of erythrocytesglutathioneperoxidase.J.Lab.Clin. Med1986;40:158-69.
Racker E. Glutathione reductase from bakers’ yeast and beefliver.TheJournalofBiologicalChemistry 1955;217:855-866.
RukkumaniR,ArunaK,VarmaPS,RajasekaranKNandMenon VP. Comparative effects of curcumin and an analog ofcurcuminonalcoholandPUFAinducedoxidativestress.Journal of Pharmacy & Pharmaceutical Sciences 2004; 7: 274-283.
VijayaPadmaV,SujaRandShyamalaDeviCS.HepatoprotetiveeffectofLiv.52onAntitubercularDruginduced Hepatotoxicity in rats, Fitoterapia(LXIX) 1998; 6:520.
Saraswathy SD, Suja V, Gurumurthy and Shyamaladevi C S.Effect of Liv.100 against anti-tuberculosis drugs (isoniazid,rifampicin and pyrazinamide) induced hepatotoxicity in rats.IndianJ Pharmacol,1998,30:233.
Man-Fung Y, Takanobu K, Masashi M et al. Clinical outcomeand virologic profiles of severe hepatitis B exacerbation dueto YMDDmutations.JHepatol2003;39:850-855.
Kissler H J, Hauffen J, Hennig R, Gepp H and Schwille PO.Glucose and lipid metabolism after liver transplantation in inbred rats: Consequencesofhepaticdenervation,Metabolism2005;54:881-890.
ViswanathaSwamyAHM, RuchaV.Kulakarni, A.H.M.Thippeswamy,B.C.KotiandAparnaGore.EvaluationofhepatoprotectiveactivityofCissusquadrangularisstemextract against isoniazid induced liver damage in rats. Indianjournalofpharmacology 2010,Dec,Vol42,Issue6:397-400.
Attri S, Rana SV, Vaiphei K, Sodhi CP, Katyai R, Goel RC et al.Isoniazid andrifampicin induced oxidative hepatic injuryprotection by N-acetylcysteine. Hum ExpToxicol2000; 19:517-24.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.